Rabbit anti otx2

Manufactured by Merck Group

Sourced in Switzerland

Rabbit anti-OTX2 is a laboratory-grade antibody that specifically binds to the OTX2 protein. OTX2 is a transcription factor involved in the development of the brain and sensory organs. This antibody can be used in various research applications, such as western blotting, immunohistochemistry, and immunocytochemistry, to detect and study the expression and localization of the OTX2 protein.

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Lab products found in correlation

Axiovert 200m, by Zeiss (4 mentions) Goat anti foxa2, by Santa Cruz Biotechnology (3 mentions) Rabbit anti vglut1, by Synaptic Systems (3 mentions) Mouse anti gaba, by Merck Group (2 mentions) Mouse anti nestin, by R&D Systems (2 mentions) Rabbit anti vglut2, by Synaptic Systems (2 mentions) Rabbit anti vgat, by Synaptic Systems (2 mentions) Rabbit anti lmx1a, by Merck Group (2 mentions) Mouse anti tuj1, by Promega (2 mentions) Rabbit anti bf1, by Abcam (2 mentions) Mouse anti nkx2, by Merck Group (2 mentions) Rabbit anti glutamate, by Merck Group (2 mentions) Alexa fluor 488 for green, by R&D Systems (2 mentions) Alexa fluor 555 for red, by R&D Systems (2 mentions) Rabbit anti tuj1, by Fortrea (2 mentions) Rabbit anti pax6, by Fortrea (2 mentions) Mouse anti gfap, by Merck Group (1 mentions) Rabbit anti tbr1, by Abcam (1 mentions) Rabbit anti nestin, by Abcam (1 mentions) Mouse anti nanog, by Merck Group (1 mentions)

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Rabbit anti-

5 protocols using rabbit anti otx2

Immunofluorescence Staining of Neural Progenitor Markers

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Cells were fixed with 4% PFA for 10 min, washed with PBS, and blocked with 0.2% Triton X in PBS supplemented with 5% BSA and 10% goat/donkey serum. Cells were then incubated with primary antibodies in 0.2% Triton X in PBS with 5% BSA and 5% goat/donkey serum: rabbit anti-LMX1A (1:2,000, Millipore), goat anti-FOXA2 (1:50; Santa Cruz), rabbit anti-TH (1:500; Pel-Freez), mouse anti-TUJ1 (1:2,000; Promega), rabbit anti-TUJ1 (1:2,000; Covance), rabbit anti-OTX2 (1:1000; Millipore), mouse anti-Nestin (1:50; R&D), rabbit anti-BF1 (1:100; Abcam), mouse anti-NKX2.1 (1:200; Chemicon), rabbit anti-PAX6 (1:300; Covance), rabbit anti-Glutamate (1:2000; Sigma), rabbit anti-VGLUT1 (1:500; Synaptic Systems), rabbit anti-VGLUT2 (1:500; Synaptic Systems), mouse anti-GABA (1:100, Sigma), and rabbit anti-VGAT (1:500; Synaptic Systems).
Corresponding fluorescent-labeled secondary antibodies were used (Alexa-Fluor 488 for green, Alexa-Fluor 555 for red; R&D). Images were captured using a Carl Zeiss Axiovert 200M (Jena, Germany) microscope.

Lee C.T., Bendriem R.M, & Freed W.J. (2015). A new technique for modeling neuronal connectivity using human pluripotent stem cells. Restorative Neurology and Neuroscience, 33(3), 347-356.

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Immunohistochemical Characterization of Neuronal Cell Types

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Cells were fixed for 15 minutes using 4% paraformaldehyde with 4% sucrose in TBS (TrisHCl pH 7.5, NaCl and MilliQ), washed and permeabilized for 15 minutes with Triton-X100 (0.25%) in TBS. After 30 minutes blocking with donkey serum in TBS-Triton (0.25%), cells were incubated overnight at 4 °C with the following antibodies: rabbit or mouse anti-β3 tubulin, rabbit anti-PAX6 (all Covance), mouse anti-HuCHuD, rabbit anti-OCT4, rabbit anti-GS1 (all Thermo Fisher Scientific), chicken anti-MAP2 (Aves), rabbit anti-Nestin, rabbit anti-TBR1, rat anti-CTIP2, mouse anti-SATB2 (all Abcam), rabbit anti-vGLUT1, mouse anti-GAD65 (both Synaptic Systems), mouse anti-S100B (BD transduction laboratories), mouse anti-NANOG, rabbit anti-OTX2, or mouse anti-GFAP (all Millipore). Subsequently, cells were washed and incubated for 1 hour at room temperature with Alexa secondary antibodies (Thermo Fisher Scientific). DAPI was used to counterstain the nuclei. Images were taken either manually with a Leica DMI 4000B microscope or Zeiss LSM 510 (confocal) or automated with the C7000™ High Content Imaging System (confocal, Yokogawa) or Opera Phenix™ High Content Screening System (confocal, Perkin Elmer).

Kuijlaars J., Oyelami T., Diels A., Rohrbacher J., Versweyveld S., Meneghello G., Tuefferd M., Verstraelen P., Detrez J.R., Verschuuren M., De Vos W.H., Meert T., Peeters P.J., Cik M., Nuydens R., Brône B, & Verheyen A. (2016). Sustained synchronized neuronal network activity in a human astrocyte co-culture system. Scientific Reports, 6, 36529.

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Immunofluorescence Staining of Neuronal Markers

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Cells were fixed with 4% PFA for 10 min, washed with PBS, and blocked with 0.2% Triton X in PBS supplemented with 5% BSA and 10% goat/donkey serum. Cells were then incubated with primary antibodies in 0.2% Triton X in PBS with 5% BSA and 5% goat/donkey serum: rabbit anti-LMX1A (1:2,000, Millipore), goat anti-FOXA2 (1:50; Santa Cruz), rabbit anti-TH (1:500; Pel-Freez), mouse anti-TUJ1 (1:2,000; Promega), rabbit anti-TUJ1 (1:2,000; Covance), rabbit anti-OTX2 (1:1000; Millipore), mouse anti-Nestin (1:50; R&D), rabbit anti-BF1 (1:100; Abcam), mouse anti- NKX2.1 (1:200; Chemicon), rabbit anti-PAX6 (1:300; Covance), rabbit anti-Glutamate (1:2000; Sigma), rabbit anti-VGLUT1 (1:500; Synaptic Systems), rabbit anti-VGLUT2 (1:500; Synaptic Systems), mouse anti-GABA (1:100, Sigma), and rabbit anti-VGAT (1:500; Synaptic Systems).
Corresponding fluorescent-labeled secondary antibodies were used (Alexa-Fluor 488 for green, Alexa-Fluor 555 for red; R&D). Images were captured using a Carl Zeiss Axiovert 200M (Jena, Germany) microscope.

Lee C.T., Bendriem R.M, & Freed W.J. (2015). A new technique for modeling neuronal connectivity using human pluripotent stem cells. Restorative Neurology and Neuroscience, 33(3), 347-356.

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Immunohistochemical Analysis of Cell Markers

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After the prescribed period of graft survival (1 or 6 months), a subset of animals (n = 5/group) were killed by an overdose of sodium pentobarbitone (100 mg/kg), and transcardially perfused with 4% paraformaldehyde and cryosectioned. Immunohistochemistry was performed on fixed cell cultures or brain sections as previously described (Somaa et al., 2017 (link)). Primary antibodies and dilutions were as follows: 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL; Sigma Aldrich), goat anti-FOXA2 (1:200; Santa Cruz Biotechnology), chicken anti-GFP (1:1,000; Abcam), rabbit anti-GFAP (1:800; DAKO), mouse anti-HNA (1:300; Millipore), rabbit anti-Ki67 (1:1,000; Thermo Fisher), mouse anti-NESTIN (1:200; Millipore), mouse anti-Neuroligin 3 (Nlgn3, 1:100; Synaptic Systems), mouse anti-OCT4 (1:100; Santa Cruz), rabbit anti-Olig1 (1:200; Millipore), rabbit anti-OTX2 (1:4,000; Millipore), mouse anti-PSA-NCAM (1:200; Santa Cruz), goat anti-SOX2 (1:200; R&D), mouse anti-synaptophysin (hSYP, 1:1,000; Enzo Life Sciences), sheep anti-TH (1:800; Pelfreeze), rabbit anti-TH (1:1,000; Pelfreeze). For quantification of HNA⁺, Ki67⁺, GFP⁺, and TH⁺ cells, images were captured at 20× magnification using a Zeiss Axio Observer Z.1 epifluorescence microscope. The density of Nestin labeling (percentage of immunoreactive pixels) was assessed from captured images and analyzed using ImageJ software.

Bye C.R., Penna V., de Luzy I.R., Gantner C.W., Hunt C.P., Thompson L.H, & Parish C.L. (2019). Transcriptional Profiling of Xenogeneic Transplants: Examining Human Pluripotent Stem Cell-Derived Grafts in the Rodent Brain. Stem Cell Reports, 13(5), 877-890.

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Retinal Cell Immunofluorescence Staining

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Immunofluorescence staining of retinal sections or dissociated cells was performed as previously described (Orieux et al., 2014) . The following primary antibodies were used: mouse anti-ATP Synthase Subunit Beta (Life Technologies); mouse anti-Brn3a (Millipore, Guyancourt, France); mouse anti-Calbindin (Swant, Marly, Switzerland); mouse anti-Calretinin (Millipore); mouse anti-GFP (Clinisciences); rabbit anti-GFP (Roche-Diagnostics); rabbit anti-CRX (gift from Dr CM Craft); mouse anti-Glutamine Synthetase (GS; Millipore); mouse anti-Go (Millipore); rabbit anti-Otx2 (Millipore); rabbit anti-Pax6 (Millipore); rabbit anti-PKCα (Santa Cruz Biotechnology); rabbit anti-Recoverin (Millipore), mouse anti-Rhodopsin (R4D2, gift from Dr Molday), rabbit anti-PTPIP51 (Sigma-Aldrich). Fluorescent staining for actin was performed using Alexa Fluor-594 phalloidin (Life Technologies) and TUNEL assay was performed using the in situ cell death detection kit (Roche-Diagnostics), both completed according to the manufacturer's recommendations.

Orieux G., Slembrouck A., Bensaïd M., Sahel J.A, & Goureau O. (2015). The protein tyrosine phosphatase interacting protein 51 (PTPIP51) is required for the differentiation of photoreceptors. Neuroscience, 300.

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