Quantikine human vegf kit

Manufactured by R&D Systems

Sourced in United States

The Quantikine human VEGF kit is a quantitative sandwich enzyme immunoassay designed to measure human vascular endothelial growth factor (VEGF) concentrations in cell culture supernates, serum, and plasma. The kit utilizes a microplate pre-coated with a monoclonal antibody specific for human VEGF.

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Lab products found in correlation

Serum separator tube, by BD (1 mentions) Human igf 1 duoset, by R&D Systems (1 mentions) Quantikine human bdnf kit, by R&D Systems (1 mentions)

Close spelling variants of this product

Quantikine Human VEGF Human VEGF Quantikine Human Quantikine kits Quantikines Human kits

6 protocols using quantikine human vegf kit

VEGF Activation in MAP3K6 Variants

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MAP3K6 influences vascular endothelial growth factor (VEGF) activation in cell models. We compared resting state VEGF levels in serum samples from 11 FMs (8 MAP3K6 variant carriers and 3 noncarriers) with 12 age-matched and sex-matched patients from a dystonia cohort who had normal cognitive status, no clinical or radiologic evidence of stroke, no white matter abnormalities in MRI, no history of malignancy, and no diabetes mellitus. Analyses were performed with ELISA, using the Quantikine human VEGF kit (R&D Biosystems) according to the manufacturer's instructions and as used in clinical diagnostics practice at the Department for Clinical Immunology and Transfusion Medicine, Region Skåne, Sweden. VEGF levels were performed in duplicate, and the Mann Whitney U test was used for statistical analyses.
Written informed consent was obtained from all patients participating in the study according to the Declaration of Helsinki. The study was approved by the Regional Ethical Review Board in Lund.

Ilinca A., Englund E., Samuelsson S., Truvé K., Kafantari E., Martinez-Majander N., Putaala J., Håkansson C., Lindgren A.G, & Puschmann A. (2021). MAP3K6 Mutations in a Neurovascular Disease Causing Stroke, Cognitive Impairment, and Tremor. Neurology: Genetics, 7(1), e548.

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VEGF Quantification in Serum

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Blood samples were processed within the hour after collection. Peripheral blood was centrifuged at 2500 rpm for 10 minutes. Serum was collected and stored at −80°C until analysis, for a period not exceeding 6 months. Samples were thawed only once. Serum concentrations of VEGF were measured by specific ELISA assays (designed to measure human VEGF-165 or VEGF-A) using the Quantikine Human VEGF kit (R&D Systems) according to the manufacturer's protocol. Each sample was measured in duplicate; intra-assay variation among the duplicates for all samples was <10%. The limit of detection of the assay was 9 pg/mL.

Ramos C., Napoleão P., Selas M., Freixo C., Viegas Crespo A.M., Mota Carmo M., Cruz Ferreira R, & Pinheiro T. (2014). Prognostic Value of VEGF in Patients Submitted to Percutaneous Coronary Intervention. Disease Markers, 2014, 135357.

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Quantifying Extracellular VEGF under Hypoxia

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Quantikine human VEGF kit was purchased from R&D systems (Minneapolis, MN). Cells were seeded in 12-well plates. At 24 h post-transfection, the medium was replaced by DMEM/F12, and cells were cultured under hypoxia or normoxia for another 24 h. Then the supernatants were collected, and extracellular VEGF in the medium was quantified according to the manufacturer’s instructions.

Liao Y., Lu W., Che Q., Yang T., Qiu H., Zhang H., He X., Wang J., Qiu M., Zou Y., Gu W, & Wan X. (2014). SHARP1 Suppresses Angiogenesis of Endometrial Cancer by Decreasing Hypoxia-Inducible Factor-1α Level. PLoS ONE, 9(6), e99907.

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Serum Biomarkers in Intervention Study

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Eight milliliters of blood were collected from the antecubital vein of each subject before and after 16 weeks of intervention, using a 22-gauge needle and a serum separator tube (Becton Dickinson, Franklin Lakes, NJ, USA). Collected blood samples were centrifuged for 15 min at 3000 rpm and then stored at −80 °C until analysis. The analysis of serum BDNF, VEGF, and IGF-1 levels was performed using sandwich enzyme-linked immunosorbent assay (ELISA). For BDNF, we used the human BDNF Quantikine Kit (Catalog No. DBD00; R&D Systems, Minneapolis, MN, USA); for VEGF, we used the human VEGF Quantikine Kit (Catalog No. DVE00; R&D Systems), and for IGF-1, we used the human IGF-1 DuoSet (Catalog No. DY291; R&D Systems). Fluorescence was measured at 450 nm with a microplate reader (Emax; Molecular Devices, Sunnyvale, CA, USA).

Cho S.Y., So W.Y, & Roh H.T. (2017). The Effects of Taekwondo Training on Peripheral Neuroplasticity-Related Growth Factors, Cerebral Blood Flow Velocity, and Cognitive Functions in Healthy Children: A Randomized Controlled Trial. International Journal of Environmental Research and Public Health, 14(5), 454.

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VEGF Secretion Quantification in MM Cells

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VEGF levels secreted by MM cells treated with EZN-2968 or scrambled oligonucleotide (20 μmol/L) were quantified using Human VEGF Quantikine® kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. Cell cultures media were harvested for up to 96h. All standards and samples were tested in triplicate.

Borsi E., Perrone G., Terragna C., Martello M., Dico A.F., Solaini G., Baracca A., Sgarbi G., Pasquinelli G., Valente S., Zamagni E., Tacchetti P., Martinelli G, & Cavo M. (2014). Hypoxia inducible factor-1 alpha as a therapeutic target in multiple myeloma. Oncotarget, 5(7), 1779-1792.

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VEGF Secretion Assay in Prostate Cancer Cells

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To analyze VEGF secretion, PC-3 and LNCaP cells were seeded in 6-well plates for 24 h. These cells were then serum starved for 12 h and treated with 1640 medium supplemented with 2% FBS, in the presence or absence ofAs₂O₃ for 48 h. The conditioned media was collected, cleared by centrifugation and stored at −80°C. ELISA was performed using the human VEGF Quantikine kit (R&D Systems, Minneapolis, USA) according to company protocol. Recombinant human VEGF was used for calibration.

Ji H., Li Y., Jiang F., Wang X., Zhang J., Shen J, & Yang X. (2014). Inhibition of transforming growth factor beta/SMAD signal by MiR-155 is involved in arsenic trioxide-induced anti-angiogenesis in prostate cancer. Cancer Science, 105(12), 1541-1549.

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