Bio plex pro human chemokine assay kit

Manufactured by Bio-Rad

Sourced in United States

The Bio-Plex Pro Human Chemokine assay kit is a multiplex immunoassay that allows for the simultaneous quantification of multiple chemokines in biological samples. The kit utilizes magnetic bead-based technology and fluorescent-coded beads to enable the detection and measurement of target analytes.

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Lab products found in correlation

24 well plate, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Bio-Plex (286 citations) Bio-Plex assay (73 citations) Bio-Plex kit (41 citations) Bio-Plex Pro Human Chemokine Assay (16 citations) Bio-Plex Pro Human Chemokine kit (4 citations) Bio-Plex ProTM Human Chemokine assay (2 citations) Human Bio-Plex Pro (2 citations) Bio-Plex Pro Human Chemokine Panel 40-plex assay kit (2 citations)

3 protocols using bio plex pro human chemokine assay kit

Aa OMP29 Modulation of LPS-Induced CXCL-8

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The confluent monolayers of OBA-09 cell, as previously described, were added to 24 well/plates (ThermoFisher, Carlsbad, CA, United States) and challenged with E. coli serotype O111:B4 LPS (500 ng/ml; Sigma–Aldrich). Then, aliquots of 1 μg/ml and 10 μg/ml of Aa OMP29^His were added to each well. After 4 h of interaction, levels of CXCL-8 in free cell supernatant were established by Bio-Plex Pro Human Chemokine assay kit (Bio-Rad, United States), following the manufacturer's instructions. Those levels were determined by comparison to the standard curve (mean of fluorescence intensity vs. pg/ml), using Bio-Plex software manager 4.0. The results were shown in percentage with CXCL-8 concentration (pg/ml) of the 4 h cultured with OBA-09 being 100% and statistical analysis was performed in GraphPad Prism version 6.0 (GraphPad Software).

da Silva M.P., Silva V.D., Pasetto S., Ando-Suguimoto E.S., Kawamoto D., Mata G.M., Murata R.M., Mayer M.P, & Chen C. (2022). Aggregatibacter actinomycetemcomitans Outer Membrane Proteins 29 and 29 Paralogue Induce Evasion of Immune Response. Frontiers in Oral Health, 3, 835902.

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Saliva Chemokine and Cytokine Profiling

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Unstimulated whole saliva samples were obtained. The levels of chemokines and cytokines in the saliva samples were evaluated by a Bio-Plex Pro™ Human Chemokine assay kit (Bio-Rad, Hercules, CA, USA) following the instructions of the manufacturer, as described in http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6499.pdf. Detailed information on these procedures was previously described [27 (link)].

Kawamoto D., Borges R., Ribeiro R.A., de Souza R.F., Amado P.P., Saraiva L., Horliana A.C., Faveri M, & Mayer M.P. (2021). Oral Dysbiosis in Severe Forms of Periodontitis Is Associated With Gut Dysbiosis and Correlated With Salivary Inflammatory Mediators: A Preliminary Study. Frontiers in Oral Health, 2, 722495.

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Multiplex Chemokine Quantification in Plasma

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The multiplex assay was completed using patient undiluted plasma and serum. Plasma chemokine levels were quantified using the Bio-Plex Pro™ Human Chemokine Assay Kit (Bio-Rad) that was custom designed to measure the following chemokines and cytokines: CCL2/MCP-1, CXCL8/IL-8, CXCL9/MIG, CXCL1/Gro-α, IL-10. The assay was carried out following manufacturer’s instructions, and the results were analyzed using the Bio-Plex Manager Software version 6.1.1. Statistical multivariate pairwise correlation analyses with these analytes can be found in Supplemental Table 3.

Jenkins B.D., Martini R.N., Hire R., Brown A., Bennett B., Brown I., Howerth E.W., Egan M., Hodgson J., Yates C., Kittles R., Chitale D., Ali H., Nathanson D., Nikolinakos P., Newman L., Monteil M, & Davis M.B. (2019). Atypical Chemokine Receptor 1 (DARC/ACKR1) in breast tumors is associated with survival, circulating chemokines, tumor infiltrating immune cells and African Ancestry. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 28(4), 690-700.

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