Alex fluor labeled secondary antibodies

Manufactured by Thermo Fisher Scientific

Alexa Fluor-labeled secondary antibodies are fluorescently-conjugated antibodies that are used to detect and visualize specific target proteins in various laboratory applications, such as Western blotting, immunohistochemistry, and flow cytometry. These antibodies provide a sensitive and reliable way to amplify and identify the signal from primary antibodies bound to their target proteins.

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Lab products found in correlation

Rat anti mouse cd102 icam2, by BD (2 mentions) Mouse anti twist1, by Santa Cruz Biotechnology (1 mentions) Rabbit cleaved caspase 3, by Cell Signaling Technology (1 mentions) Rat anti endomucin, by Santa Cruz Biotechnology (1 mentions) Goat anti gfp fitc, by Abcam (1 mentions) Rat anti mouse pecam 1 cd31, by BD (1 mentions) Mouse anti smooth muscle actin, by Merck Group (1 mentions) Goat anti ephb4, by R&D Systems (1 mentions) Rabbit anti dsred, by Takara Bio (1 mentions) Isolectin gs ib4, by Thermo Fisher Scientific (1 mentions) Texas red streptavidin, by Vector Laboratories (1 mentions) Chicken anti beta galactosidase, by Abcam (1 mentions)

Close spelling variants of this product

Alex-fluor secondary antibodies Labeled secondary antibodies

2 protocols using alex fluor labeled secondary antibodies

Immunofluorescence Staining of Rat Tissues

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Rat anti-endomucin (Santa Cruz, sc-65495), goat anti-GFP FITC (Abcam, ab6662), rabbit anti-DsRed (Clontech 632496), rat anti-mouse CD102/ICAM2 (BD Biosciences, 553326), mouse anti-Twist1 (Santa Cru z, sc-81417), rat anti-mouse CD31/PECAM-1 (BD pharmingen 553370), rabbit anti-Sox17 (Zhou et al., 2015), mouse anti-smooth muscle actin (Sigma-Aldrich; C6198), rabbit anti-Runx2 (Abeam abl92256), rabbit cleaved caspase-3 (Cell Signaling 9661) and goat anti-EphB4 (R&D systems AF446). Alex Fluor-labeled secondary antibodies were from Invitrogen. See STAR Methods chart.

Tischfield M.A., Robson C.D., Gilette N.M., Chim S.M., Sofela F.A., DeLisle M.M., Gelber A., Barry B.J., MacKinnon S., Dagi L.R., Nathans J, & Engle E.C. (2017). Cerebral vein malformations result from loss of Twist1 expression and BMP signaling from skull progenitor cells and dura. Developmental cell, 42(5), 445-461.e5.

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Immunohistochemistry Protocol for Brain Vasculature

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Antibodies used in this study were as follows: chicken anti-beta-galactosidase (Abcam 9361); rabbit anti-GLUT-1 (Thermo Scientific RB-9052-P0); rabbit anti-NG2 (Millipore AB5320); rat anti-mouse CD102/ICAM2 (BD Biosciences 553326); rat anti-PLVAP/ MECA-32 (BD Biosciences 553849); mouse anti-Claudin-5, Alexa 488 conjugate (Invitrogen 352588), and Texas Red streptavidin (Vector Laboratories SA-5006). AlexFluor-labeled secondary antibodies and GS lectin (Isolectin GS-IB4) were from Invitrogen. Primary antibodies were used at 1:200 to 1:500 dilution.

Zhou Y, & Nathans J. (2014). Gpr124 controls CNS angiogenesis and blood-brain barrier integrity by promoting ligand-specific canonical Wnt signaling. Developmental cell, 31(2), 248-256.

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