Acetylcholine chloride (ACh-Cl) (Sigma-Aldrich, St. Louis, MO, USA) was used as a standard sample and acetylcholine-d4 chloride (d4-ACh) (FUJIFILM WAKO Pure Chemical) was used as an internal standard (IS) for calibration. An aqueous solution containing 1000 μg/mL of ACh-Cl or ACh-d4 was prepared as a stock solution. A series of 0.14, 0.41, 1.23, 3.7, 11.1, 33.3, and 100 ng/mL standard samples containing 1 ng/mL ACh-d4 were prepared from the stock solution. Each solution (100 μL) was placed into a 1.5 mL sample tube, to which methanol (250 μL) and chloroform (200 μL) were added. The resulting mixture was vortexed for 1 min and centrifuged at 15,000 rpm for 10 min at 4 °C. After centrifugation, 180 μL of the supernatant was collected in an autosampler vial, 20 μL of which was injected into the LC–MS/MS system. The calibration curve was generated by plotting the nominal concentrations of ACh against the peak area ratio of ACh-Cl to IS.
Acetylcholine chloride ach cl
Manufactured by Merck Group
Sourced in United States
Acetylcholine chloride (ACh-Cl) is a chemical compound used in various laboratory and research applications. It is a salt of acetic acid and choline, serving as a precursor for the neurotransmitter acetylcholine. ACh-Cl is frequently employed in experiments involving the study of neurological and physiological processes.
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Lab products found in correlation
2 protocols using acetylcholine chloride ach cl
1
Quantification of Acetylcholine by LC-MS/MS
2
Neuronal Responses to Pharmacological Agents
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GM was purchased from Standard Technology Co., Ltd (Shanghai, China). The purity of the synthetic GM was ≥98%, as assessed by HPLC. The compound was dissolved and stored in dimethyl sulfoxide (DMSO) to produce 20 mmol/L stock solutions that were then diluted in bath solution to obtain the final concentrations. DMSO at the final concentrations (≤0.5%) was well tolerated with no observable toxic effects to the neurons. To conduct research on animals, GM was dissolved in a mixture of 5% DMSO and 95% Tween-80. TTX was purchased from Aquaculture Technical Developing Company, Qinhuangdao, China. Topiramate (TPM), levetiracetam (LEV), γ-amino butyric acid (GABA), N-methyl-D-aspartic acid (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA), kainic acid (KA) and acetylcholine chloride (ACh-Cl) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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