GC cell lines, the differentiated type (AGS, IM95, MKN1, MKN7, MKN74 and N87) and the undifferentiated type (GCIY, KATO-III, MKN45, NUGC2, NUGC3, NUGC4, OCUM1 and SC-6-JCK), were obtained from the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan) or the American Type Culture Collection (ATCC, Manassas, VA, USA). A control, non-tumorigenic epithelial cell line (FHs 74) was purchased from the ATCC. The cells were cultured at 37 °C in RPMI medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% foetal bovine serum in an atmosphere containing 5% CO2. All cell lines were authenticated using the short tandem repeat PCR method by the Japanese Collection of Research Bio Resources Cell Bank before the study commenced.
Ocum 1
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
OCUM-1 is a laboratory instrument utilized for culturing and maintaining cell lines. It provides a controlled environment for cell growth, offering temperature, humidity, and gas regulation. The core function of OCUM-1 is to support the development and preservation of various cell types used in research and development.
Automatically generated - may contain errors
Lab products found in correlation
Mkn74, by Japanese Collection of Research Bioresources Cell Bank (6 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Mkn45, by Japanese Collection of Research Bioresources Cell Bank (5 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (5 mentions)
Nugc4, by Japanese Collection of Research Bioresources Cell Bank (4 mentions)
Kato 3, by Japanese Collection of Research Bioresources Cell Bank (4 mentions)
Nugc3, by Japanese Collection of Research Bioresources Cell Bank (4 mentions)
Kato 3, by American Type Culture Collection (4 mentions)
Nugc2, by Japanese Collection of Research Bioresources Cell Bank (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Sc 6 jck, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Nugc 4, by RIKEN BioResource Center (2 mentions)
Nci n87, by American Type Culture Collection (2 mentions)
Rpmi medium, by Thermo Fisher Scientific (1 mentions)
Fhs74, by American Type Culture Collection (1 mentions)
Mkn45, by American Type Culture Collection (1 mentions)
Rpmi 1640 glutamaxtm 1 medium, by Thermo Fisher Scientific (1 mentions)
Dmem low glucose, by GE Healthcare (1 mentions)
Neratinib, by Selleck Chemicals (1 mentions)
10 protocols using ocum 1
1
Gastric Cancer Cell Line Characterization
2
Gastric Cancer Cell Lines and Tissues
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The GC cell lines GCIY, IM95, MKN1, MKN7, MKN45, MKN74, NUGC2, NUGC3, NUGC4, OCUM-1, and SC-6-JCK were obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan). AGS, KATOIII, and N87 GC cell lines and a nontumorigenic epithelial cell line (FHs74) were acquired from the American Type Culture Collection (Manassas, VA, USA). Three hundred pairs of surgically resected GC and adjacent noncancerous tissues were obtained from patients who underwent gastrectomy. A freely available integrated dataset (n = 1065 GC patients) was accessed at http://kmplot.com/analysis/ [13 (link)].
3
Cultivation of Gastric Cancer Cell Lines
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Human GC cell lines OCUM-1 and MKN-74 were purchased from the Japanese Collection of Research Bioresources (JCRB) Cell Bank, whereas NCI-N87 (N87), AGS, KATO-III and MKN-45 cells were acquired from ATCC. Cells were maintained with 10% heat-inactivated FBS (Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin-streptomycin (10,000 Units/mL penicillin; 10,000 mg/mL streptomycin; Thermo Fisher Scientific, Waltham, MA, USA) supplemented RPMI 1640+GlutaMAXTM-I medium (Thermo Fisher Scientific, Waltham, MA, USA). OCUM-1 cells were grown with low-glucose DMEM (GE Healthcare Life Sciences, Chicago, IL, USA). Cell lines were cultured at 37 °C in a 5% CO2 humidified atmosphere.
4
Gastric Cancer Cell Line Panel for Targeted Therapies
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Twelve gastric cancer cell lines (ECC10, GCIY, KATO‐III, MKN7, MKN74, NCI‐N87, NUGC3, NUGC4, OCUM‐1, SH‐10‐TC, SNU‐16, and SNU‐216) were used in this study. ECC10, GCIY, and MKN7 were provided by Riken BRC through the National Bio‐Resource Project of Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. NUGC3, MKN74, and OCUM‐1 were provided by Japanese Collection of Research Bioresources/Health Science Research Resources Bank (JCRB/HSRRB), Osaka, Japan. KATO‐III, NCI‐N87, NUGC4, SNU‐16, SH‐10‐TC were purchased from ATCC (Manassas, VA, USA), and SNU‐216 was obtained from the Korean Cell Line Bank. All the cells, other than GCIY and OCUM‐1, were cultured in RPMI‐1640 media supplemented with 10% FBS. GCIY and OCUM‐1 were cultured in minimum essential media (Sigma‐Aldrich) with 15% FBS and DMEM with 0.5 mmol/L sodium pyruvate and 10% FBS, respectively. Afatinib, neratinib, and PPP were purchased from Selleckchem and MedChem Express. Gefitinib was purchased from Sykkinase. Trastuzumab and pertuzumab were obtained from Chugai Pharmaceutical Co.
5
Cisplatin Dose Response in Cancer Cell Lines
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AZ521 and OCUM-1 were obtained from Japanese Collection of Research Bioresources, Japan. SNU610 and SNU719 were purchased from Korean Cell Line Bank, Korea. All the cell lines were cultured at 37 °C in a humidified atmosphere containing 5 % CO2 and maintained in RPMI 1640 medium (Gibco), containing 10 % heat inactivated fetal bovine serum (Gibco) and 1 % penicillin/streptomycin (Gibco). For dose response studies, the cell lines were treated with cisplatin at IC50 concentration.
6
Gastric Cancer Cell Line Characterization
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The following human GC cell lines were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan): MKN45 (JCRB0254); the luciferase stably expressing cell line MKN45-Luc (JCRB1379), NUGC-2 (JCRB0821), OCUM-1 (JCRB0192), and KATO-III (JCRB0611). AGS was purchased from the American Type Culture Collection (Manassas, VA, USA). Cell line identities were confirmed by DNA fingerprinting through short tandem repeat profiling.
7
Culturing Human Gastric Cancer Cell Lines
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Two human gastric cancer cell lines, NUGC-4 and MKN45, were purchased from the RIKEN BioResource Center (Tsukuba, Japan). The KATOIII and OCUM-1 human gastric cancer cell lines were obtained from the Japanese Collection of Research Bioresources (Tokyo, Japan). All cells were cultured in RPMI 1640 containing 10% heat-inactivated fetal bovine serum (FBS), 2 mM L-glutamine, 100 μg/ml streptomycin and 200 U/ml penicillin (GIBCO-BRL, Gaithersburg, MD, USA).
8
Cell Line Authentication and Culture
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MKN7, MKN45, OCUM-1, and NUGC-3 were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). NCI-N87 was purchased from the American Type Culture Collection (Manassas, VA, USA), and ECC10, GSU, HGC27, KE39, and NUGC-4 were purchased from the RIKEN BioResource Research Center (Ibaraki, Japan). These cell lines have no gene amplification or deletion for PD-L1 and PD-L2 according to the cell line data from each company. For PCR, KATO III was purchased from the American Type Culture Collection and OE19 was purchased from the Merck KGaA (Darmstadt, Germany). All cell lines, in which the absence of mycoplasma was confirmed, were cultured in RPMI-1640 containing l-glutamine (Merck KGaA) with 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific, Inc.), and were verified as authentic through short tandem repeat profiling.
9
Establishing Human Gastric Cancer Cell Lines
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Human gastric cancer cell lines MKN-1, MKN-45, MKN-74, NUGC-4, OCUM-1, and Kato III were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan). Vero (African green monkey kidney) cells were purchased from the American Type Culture Collection (Rockville, MD, USA). Cells were cultured according to the directions provided by the suppliers. Human scirrhous gastric cancer cell lines 44As3, luciferase-expressing 44As3 (44As3Luc), HSC-60, and HSC-39 were established and cultured as previously described.20 (link),50 (link) Luciferase-expressing MKN45 (MKN45-luc) cells were generated with pre-made luciferase lentiviral particles expressing the firefly luciferase3 gene (#LVP326, GenTarget) according to the manufacturer’s protocol. Clonal selection was performed in a medium containing 10 μg/mL blasticidin (Wako, Japan).
10
Gastric Cancer Cell Line Culture
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Mainly, human gastric cancer cell lines, MKN45 and KATO-III, were used in this study. MKN45 cell lines were obtained from Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan). KATO-III cells were provided from the department of Medical Oncology and Hematology, Sapporo Medical University School of Medicine (Hokkaido, Japan). Ten other types of human gastric cancer cells, namely MKN1, MKN74, IM95, OCUM-1, GCIY, HGC-27, NUGC-4, AGS, HSC-39, and JRST, were obtained from the JCRB Cell Bank (Osaka, Japan). Cells were maintained in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO) containing 10% FBS (Life Technologies, Carlsbad, CA) at 37 °C in an incubator with 5% CO 2 . 5-FU, cisplatin, and docetaxel were obtained from Sigma-Aldrich.
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