Cells were stained with fluorochrome-labeled antibodies according to manufacturer information (listed in Supplementary Table 3 ). To block free Fc receptors murine CD16/32 or human Fc Receptor Binding Inhibitor Polyclonal Antibody (eBioscience) were applied. Dead cells were excluded by DAPI (1 µg/ml) (Sigma Aldrich) staining. General gating strategies for surface marker expression and viability assessment are shown in Supplementary Fig. 5 . Flow Cytometry was performed using a FACS Canto II cytometer (BD Bioscience). Data were analyzed with the FlowJoTM software version 10.7.1 (BD Bioscience).
Human fc receptor binding inhibitor polyclonal antibody
Manufactured by Thermo Fisher Scientific
The Human Fc Receptor Binding Inhibitor Polyclonal Antibody is a laboratory reagent designed to inhibit the binding of human Fc receptors. It is a polyclonal antibody produced in a suitable animal host and purified for research use.
Automatically generated - may contain errors
Lab products found in correlation
Facscanto 2 cytometer, by BD (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions)
Cd3 fitc, by BioLegend (1 mentions)
Cd11c pe, by BioLegend (1 mentions)
Cd62l apc, by BioLegend (1 mentions)
Cd19 pe cy7, by BioLegend (1 mentions)
Cd25 pe, by BioLegend (1 mentions)
Cd4 apc cy7, by BioLegend (1 mentions)
Cd14 apc cy7, by BioLegend (1 mentions)
Cd5 apc, by BioLegend (1 mentions)
Pd1 pe cy7, by BioLegend (1 mentions)
Hla dr pe cy7, by BioLegend (1 mentions)
2 protocols using human fc receptor binding inhibitor polyclonal antibody
1
Fluorescent Antibody Staining Protocol
2
Multiparametric Flow Cytometry Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were stained with fluorochrome-labeled antibodies according to the manufacturer’s instructions (Staining 1: CD19 PE-Cy7 and CD5 APC; Staining 2: CD3 FITC, CD4 APC-Cy7, CD8 Amcyan, PD1 PE-Cy7, CD25 PE, CD62L APC; staining 3: CD11c PE, CD14 APC-Cy7, HLA-DR PE-Cy7, CD56 PerCP710, all Biolegend). To block free Fc receptors human Fc receptor binding inhibitor polyclonal antibody (eBioscience) were added 10 minutes before labeled antibodies. Dead cells were excluded by DAPI (1 μg/mL) (Sigma Aldrich) staining. Flow cytometric analysis was performed using a FACS Canto II cytometer (BD Bioscience). Data were analyzed with the FlowJo software version 10.7.1 (BD Bioscience).
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