Nalm6

The erythroleukemia cell line K562 and the mouse melanoma cell line B16 were purchased from the Japanese Collection of Research Bioresources cell bank (Osaka, Japan). The CD19⁺ B-cell leukemia cell line NALM6 and the mouse colon carcinoma cell line Colon-26 were obtained from the Cell Resource Center for Biomedical Research, Tohoku University (Sendai, Japan). The A375 melanoma, PG13 retroviral packaging cell line, and AsPC1 pancreatic cancer cell line were obtained from the American Type Culture Collection (Manassas, VA, USA). The Plat-A and Plat-E packaging cell lines were kindly provided by Dr. T. Kitamura (University of Tokyo, Tokyo, Japan). K562, NALM6, Colon 26, AsPC1, and their derivatives were cultured in RPMI-1640 (Nacalai Tesque, Japan) containing 10% fetal bovine serum (FBS) (Nichirei Biosciences, Tokyo, Japan). A375 and PG13 cells were cultured in DMEM (Nacalai Tesque, Kyoto, Japan) containing 10% FBS. B16 cells were cultured in MEMα (Nacalai Tesque) supplemented with 10% FBS. A375 cells were transduced with CD19 and mesothelin to generate A375-CD19 and A375-mesothelin, respectively.

OP9 (RCB1124), TSt-4 (RCB2116), NIH 3T3 (RCB2767), NALM6 (RCB1933), and K562 (RCB0027) cells were purchased from RIKEN Bio Resource Center (Tsukuba, Ibaraki, Japan) within 10 years. OP9, TSt-4, and NIH 3T3 cells were cultured in minimum essential medium-alpha modification (αMEM; Thermo Fisher Scientific) supplemented with 20% FBS (Sigma-Aldrich) and 1% penicillin-streptomycin (Nacalai Tesque). NALM6 and K562 cells were cultured in RPMI1640 (Nacalai Tesque) supplemented with 10% FBS and 1% penicillin–streptomycin as described previously (13 (link)). OP9-hDLL1 and K562-hCD19 cells have been described previously (11, 26 (link)). HEK293T and Expi293F cells were purchased from (ATCC) and Thermo Fisher Scientific within 10 years, respectively. These cells were cultured in DMEM (Nacalai Tesque) supplemented with 10% FBS and 1% penicillin–streptomycin as described previously (1 (link)). All cell lines were regularly tested for Mycoplasma contamination using BioMycox Mycoplasma PCR Detection Kit (CellSafe) and were frozen down at early passages (<7) and used in the experiments within five passages after thawing. These cells were not further authenticated by our laboratory; however, routine confirmation of in vitro growth properties, morphology, and transfection efficiency (HEK293T and Expi293F) provided evidence of correct cell identity.