The rat insulin enzyme-linked immunosorbent assay kit (ELISA, Mercodia, and Uppsala, Sweden) was used to determine serum insulin concentration. The sensitivity of the insulin assay is 0.15 μg/L with intra- and inter-assay coefficients of variations of 4.1 and 7.8%, respectively. Rat commercial hormonal kits (ZellBio GmbH company in Germany) and the ELISA method were used to measure levels of testosterone with intra- and interassay coefficients of variations of 5.1 and 9.6%, respectively; progesterone with intra- and interassay coefficients of variations of 5.9 and 10.8%, respectively; estrogen with intra- and interassay coefficients of variations of 6.2 and 11.1%, respectively; LH with intra- and interassay coefficients of variations of 4.7 and 8.9%, respectively; and FSH with intra- and interassay coefficients of variations of 3.8 and 7.3%, respectively. A glucose oxidase method kit (Pars Azmoon, Tehran, Iran) was also used to measure blood glucose levels with intra- and interassay coefficients of variations of 3.4 and 6.5%, respectively. The homeostatic model assessment of IR (HOMA-IR) index was computed as follows: [fasting serum glucose levels (mmol/l)], [insulin (IU/ml)]/22.5.
Glucose oxidase method kit
Manufactured by Pars Azmoon
Sourced in United States
The Glucose oxidase method kit is a laboratory product used for the quantitative determination of glucose levels in various samples. It operates based on the glucose oxidase enzymatic reaction principle, providing a reliable and accurate method for glucose analysis.
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Lab products found in correlation
7 protocols using glucose oxidase method kit
1
Rat Hormone and Glucose Assay
2
Exercise Training Effects on Inflammatory Markers
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All the measurements were obtained at baseline and after completing a 12-week exercise training protocol. Blood samples were drawn after a 12-h overnight fast and were kept at –80˚C for subsequent assay. Analyses were taken at least 48 h after the last bout of exercise. In the control group, measurements were taken at baseline and after 12 weeks. Serum insulin was measured by a commercial chemiluminescence assay kit (Cobas®, USA) (intra-Assay CV: 1.9%, inter-Assay CV: 2.6%) and serum glucose was measured by a glucose oxidase method kit (Pars Azmoon, IRAN). Serum TNF-α concentrations were determined using high-sensitivity ELISA (Biosource International, Camarillo, CA, USA). The lower limit of detection for TNF-α was 0.5 pg/ml, and the intra- and inter-assay coefficients of variation were < 7%. Soluble TNF-R1 and TNF-R2 in serum were determined using commercially available ELISA kits (Biosource Europe, Fleunes, Belgium). The minimum detectable concentrations for TNF-R1 and TNF-R2 were estimated to be 50 pg/ml and 0.1 ng/ml, respectively. The serum CXCL5 was determined in duplicate by ELISA with Duoset kit (DY254; R&D Systems, Minneapolis, MN) as recommended by the manufacturer. The ELISA system had an intra-assay coefficient of variation of 3-8% and an inter-assay coefficient of variation of 4-10%, respectively.
3
Serum Lipid Profile in Rats
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Five rats were chosen from each group every two weeks and were deeply anesthetized with ether (Merck). Blood sampling was provided directly from the animal heart and the spurting blood was collected in clean centrifuge tubes and allowed to clot for an hour at room temperature. It was then centrifuged at a rate of 12,000 revolutions per minute (rpm) for 10 min. The obtained clear serum was separated and labeled for the analysis. The serum levels of glucose were measured by glucose oxidase method kit (Pars Azmoon, Tehran, Iran) using blood chemical analyzer (Vitalab Selectra E, UK) and its total cholesterol and triglycerides by Enzymatic colorimetric, LDL, HDL were measured using standard biochemical kits by enzymatic cholesterol assay (Pars Azmoon, Tehran, Iran).
4
Exercise Influence on Insulin Resistance
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Blood samples (10 mL) from the antecubital vein in a sitting position were collected 24 hours before exercise protocol and 48 hours after the last session of training program in 12 hours of fasting state.
FBG was measured using glucose oxidase method kit (Pars Azmoon, Tehran, Iran), through auto-analyzer devices (Hitachi®, model 704, 902, Tokyo, Japan). Serum insulin concentrations were determined by enzyme-linked immunosorbent assay (ELISA) technique using a microplate reader. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated by computing the following equation: (fasting glycemia [mmol/L]×fasting insulin [mIU/L])/22.5 [25 (link)]. Participants who used insulin injection were excluded for the HOMA-IR analysis.
FBG was measured using glucose oxidase method kit (Pars Azmoon, Tehran, Iran), through auto-analyzer devices (Hitachi®, model 704, 902, Tokyo, Japan). Serum insulin concentrations were determined by enzyme-linked immunosorbent assay (ELISA) technique using a microplate reader. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated by computing the following equation: (fasting glycemia [mmol/L]×fasting insulin [mIU/L])/22.5 [25 (link)]. Participants who used insulin injection were excluded for the HOMA-IR analysis.
5
Omentin-1 and Metabolic Biomarkers
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All baseline measurements were performed prior to the beginning of the protocol, and post testing evaluations were made 48 h after the last session of the training program. Omentin-1 serum level was analyzed by a commercial ELISA kit (Eastbiopharm, Hangzhou, China). The intra-assay and inter-assay coe cients of variation were less than 12%, and sensitivity of the assay was 1.03 ng/mL. The serum values of HDL-C, TG, and TC concentrations were measured by an enzymatic colorimetric method using biochemical Auto-analyzer Prestige 24i (Made in Japan). LDL-C level was estimated using the Friedewald formula when serum TG was < 400 mg/dL. The glucose level was determined using the glucose oxidase method kit (Pars Azmoon, Tehran, Iran). Insulin concentration was assayed using the chemiluminescence method (LIAISON®, Germany). The Homeostasis model assessment for insulin resistance (HOMA-IR) was used to calculate insulin resistance, and it was estimated from fasting glucose and insulin, according the following equation: [Fasting glucose (mg/dl) × fasting insulin (U/l)/405] [15] (link).
6
Insulin Resistance Evaluation via Exercise
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Twenty-four hours prior to the exercise protocol and 48 h following the final session blood samples (10 mL) from the antecubital vein were collected in a sitting position with a 12-hour fasting state. Measuring FBG was carried out using a glucose oxidase method kit (Pars Azmoon, Iran) using autoanalyzer devices (Hitachi®, 704, 902, Japan). To measure serum insulin concentration, enzyme-linked immunosorbent assay (ELISA) was used with a microplate reader. To determine homeostasis model assessment of insulin resistance (HOMA-IR), the following formula was used: (fasting glycemia [mmol/L]×fasting insulin [mIU/L])/22.5 [43 (link)]. Those who had used insulin injection did not undergo the HOMA-IR analysis. HbA1c was measured by immunoturbidometry. Systolic (SBP) and diastolic blood pressure (DBP) were determined using a digital sphygmomanometer after 15 min of rest in stable conditions.
All measures were assessed 24 h before treatment (baseline) and 48 h following the intervention (follow-up). A research assistant blinded to the grouping, carried out all the baseline and following measurements.
All measures were assessed 24 h before treatment (baseline) and 48 h following the intervention (follow-up). A research assistant blinded to the grouping, carried out all the baseline and following measurements.
7
Fasting Metabolic Biomarkers
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Patients were visited after an overnight fasting of 10–14 hours. Past medical history of patients was assessed. Patients’ sera were collected at the end of the experimental period to analysis biochemical parameters. Biochemical measurements of serum glucose, lipids, and glycated hemoglobin (HbA1c) were performed. Glucose was measured by glucose oxidase method kit (Pars Azmoon, Tehran, Iran). Serum total cholesterol and triglycerides were measured using standard biochemical kits (Pars Azmoon, Tehran, IR Iran). Blood glycated hemoglobin levels were measured by using Elisa kit (Bioassay technology laboratory, Elisa kit).
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