For western blotting, mouse anti-p63 (4A4) (LabVision, Kalamazoo, MI, USA; 1/1000 dilution), mouse anti-β-actin (1/1000 dilution), rabbit anti-SDF1 (1/1500 dilution) (Cell Signaling, Boston, MA, USA), rabbit anti-CXCR4 (1/500) (Abcam, Cambridge, MA, USA) antibodies were used. For immunofluorescence experiments, rabbit anti-CXCR4 (1/500) (Abcam, Cambridge, MA, USA) (Figure 1C ) or mouse anti-CXCR4 (R&D Systems, Minneapolis, MN, USA; 8μg/ml dilution) (Supplemental Figure 2B ) antibodies were used. Secondary antibodies used were anti-mouse and anti-rat IgG horseradish peroxidase-conjugated for immunoblot and goat anti-rabbit-alexafluor-555 or goat anti-mouse-alexafluor-488 conjugated antibodies for immunofluorescence.
Mouse anti cxcr4
Manufactured by R&D Systems
Sourced in United States
Mouse anti-CXCR4 is a primary antibody that recognizes the CXCR4 chemokine receptor. CXCR4 is a G protein-coupled receptor that binds the chemokine CXCL12 and plays a role in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti β actin, by Cell Signaling Technology (1 mentions)
Rabbit anti cxcr4, by Abcam (1 mentions)
Alexa fluor 488 donkey anti goat, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Rabbit anti β catenin, by Cell Signaling Technology (1 mentions)
Rabbit anti cdx2, by Abcam (1 mentions)
Mouse anti cd44, by BD (1 mentions)
Goat anti epha2, by R&D Systems (1 mentions)
Rabbit anti cd133, by Abcam (1 mentions)
2 protocols using mouse anti cxcr4
1
Western Blot and Immunofluorescence Antibody Conditions
2
Immunofluorescence staining of cancer stem cell markers
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For immunofluorescence staining on tissue sections, CCSCs, mCCSCs and cCCSCs cells antibodies/antisera used were: mouse anti-Human Nuclei (1:100, Millipore-Merk KGaA, Darmstadt, Germany), mouse anti-BMI1 (1:100, Merk Millipore), rabbit anti-EpCAM (1:50, Cell Signaling, Beverly, MA, USA), rabbit anti-ALDHA1 (1:50, Cell Signaling), rabbit anti-bCatenin (1:50, Cell Signaling), rabbit anti-CD133 (1:100, AbCam, Cambridge, UK), rabbit anti-OLFM4 (1:200, AbCam), rabbit anti-CDX2 (1:100, AbCam), rabbit anti-CK20 (1:100, Abcam), rabbit anti-Lgr5 (1:50, Merk SigmaAldrich), rabbit anti-Laminin (1:400, Merk SigmaAldrich), mouse anti-Vimentin (1:100, Agilent, Santa Clara, CA, USA), mouse anti-HLA-ABC (1:100, Agilent), goat anti-EphA2 (1:50, R&D System, Minneapolis, MN, USA), mouse anti-CXCR4 (1:100, R&D System), rabbit anti-KI67 (1:200, Leica Microsystem, Wetzlar, Germany), mouse anti-CD44 (1:50, BD Biosciences, San Jose, CA, USA), rabbit anti-Wnt5a (1:50, LS Biosciences, Seattle WA, USA), mouse anti-MUC2 (1:50, Novusbio, Littleton, CO, USA). Goat anti mouse AlexaFluor488/546 (1:1000, Thermo Fisher Scientific, Waltham, MA, USA), goat anti rabbit AlexaFluor488 (1:1000, Thermo Fisher Scientific) and donkey anti goat AlexaFluor488 (1:1000, Thermo Fisher Scientific) secondary antibodies were used to visualize the primary antibody staining.
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