ATR-FTIR (Attenuated Total Reflection Fourier Transform Infrared) spectra were collected on a Varian 2000 FTIR spectrometer (Varian Inc, USA) using a 'GoldenGate' single reflection diamond ATR accessory (Specac Ltd, UK). 256 scans were acquired at a resolution of 2 cm -1 . Spectra were recorded at room temperature both as hydrated lipid suspension (immediately after pipetting a drop of the sample on the top of the ATR element) and as dry film spectra (after drying the sample on the ATR crystal). ATR correction was applied after each data collection. The spectral deconvolution of selected bands was performed using mixed Gaussian and Lorentzian functions. Band positions were estimated using the second derivative, band shapes were approximated by mixed Gaussian and Lorentzian function until the 2 parameter became minimal. The GRAMS/32 software package was used for all spectral manipulations.
Golden gate single reflection diamond atr accessory
Manufactured by Specac
Sourced in United Kingdom, United States
The Golden Gate single reflection diamond ATR accessory is a versatile tool designed for attenuated total reflection (ATR) spectroscopy. It features a diamond internal reflection element that allows for the analysis of a wide range of sample types, including solids, liquids, and pastes. The accessory is designed to provide a simple and efficient means of sample introduction for FTIR spectroscopy.
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Lab products found in correlation
Nicolet 6700, by Thermo Fisher Scientific (3 mentions)
Ftir scimitar series spectrometer, by Specac (2 mentions)
Varian 2000 ftir scimitar spectrometer, by Agilent Technologies (2 mentions)
Varian 2000 ftir spectrometer, by Agilent Technologies (1 mentions)
Origin software package, by OriginLab (1 mentions)
Vertex 70 ftir, by Bruker (1 mentions)
Varian 2000 ftir scimitar series, by Agilent Technologies (1 mentions)
10 protocols using golden gate single reflection diamond atr accessory
1
ATR-FTIR Analysis of Hydrated Lipid Samples
2
Attenuated Total Reflection Fourier Transform Infrared Spectroscopy Protocol
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ATR-FTIR spectra were acquired using a Varian 2000 FTIR Scimitar Series spectrometer fitted with a Golden Gate single reflection diamond ATR accessory (Specac Ltd, UK). 5 μl of sample was mounted on the diamond ATR crystal. The measurements were done at room temperature immediately after mounting (i. e. in the solution) as well as after drying the sample by slow evaporation of the buffer solvent under ambient conditions. 64 scans were co-added at a nominal resolution of 2 cm−1. Prior to spectral evaluation, the buffer background was subtracted, and all spectra were smoothened with a Savitzky-Golay convolution function. The GRAMS/32 software package (Galactic Inc, USA) was used for all spectral manipulations.
3
ATR-FTIR Spectroscopy for Polymer Analysis
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Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy measurements were conducted using a Varian 2000 FTIR Scimitar spectrometer (Varian Inc., Palo Alto, CA, USA) fitted with a liquid nitrogen-cooled mercury cadmium telluride (MCT) detector and a ‘Golden Gate’ single reflection diamond ATR accessory (Specac Ltd., Orpington, UK). A sample amount of 5 µL was dropped onto the diamond ATR surface, and the dry film spectrum was collected (at 2 cm−1 resolution and 64 scans) after the slow evaporation of the solvent under ambient conditions. Each data acquisition was followed by ATR correction. Spectral deconvolution was performed using the GRAMS/AI (7.02) spectroscopy software (Thermo Galactic, Walthman, MA, USA). Band positions for curve fitting were established using the second derivative and were fixed during the fitting procedure. Band shapes were approximated by Lorentzian functions. The intensities and the bandwidth of each component were allowed to vary until the minimal χ2 parameter was reached. After the fitting procedure, the relative contribution of a particular band component was calculated from the integrated areas of the individual components [17 (link)].
4
FTIR Spectroscopic Analysis of Samples
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FTIR spectroscopic measurements were conducted using a Varian 2000 FTIR Scimitar spectrometer (Varian Inc., Palo Alto, CA, USA) fitted with a liquid nitrogen-cooled mercury-cadmium-telluride (MCT) detector and with a ‘Golden Gate’ single reflection diamond ATR accessory (Specac Ltd., Orpington, UK). Onto the diamond ATR surface, 5 µL of the sample was mounted, and the spectrum was collected (2 cm−1 resolution and 64 scans) for a dry film after slow evaporation of the buffered solvent under ambient conditions. Each data acquisition was followed by ATR correction, and buffer subtraction and baseline corrections were performed. For peak identification, spectra were normalized by the area, and the second derivative was calculated. Spectra analysis was performed using the Origin software package (OriginLab, Northampton, MA, USA).
5
Hydrogel Characterization by FTIR
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FTIR spectroscopy was used to characterize crosslinked and sulfonated hydrogels with or without collagen absorption. The spectra of freeze-dried samples were acquired in attenuated total reflection mode (ATR) using a Nicolet 6700 (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a Golden Gate single reflection diamond ATR accessory (Specac, Orpington, UK). All spectra were recorded with 200 scans/spectrum at 4 cm−1 resolution.
6
FTIR Characterization of SLN Formulations
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FTIR spectra of SLNas, NF-SLNas, and their individual components were obtained in Attenuated Total Reflectance (ATR) using FTIR spectrophotometer (FTIR Vertex 70 Bruker, Optics, Ettlingen, Germany) equipped with a Golden Gate Single Reflection Diamond ATR accessory (Specac, Orpington, UK) in the range of 4000-600 cm -1 . Depth of light penetration was in the range 0.7-2 µm for sample of Refractive Index 1.5 at 1000 cm -1 .
7
Investigating Peptide Structure Changes via FT-IR
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Changes in the peptide structure were followed by fourier-transform
infrared spectroscopy (FT-IR). Measurements were performed by means
of a Varian 2000 (Scimitar Series) FT-IR spectrometer (Varian Inc.,
US) using a MCT (mercury-cadmium-telluride) detector and fitted with
a “Golden Gate” single reflection diamond ATR accessory
(Specac Ltd., UK). The applied attenuated total reflection (ATR) technique
allows for the study of aqueous solutions. 5 μL of the sample
was pipetted on the top of the diamond ATR element, and the spectrum
was collected immediately with 128 scans at a spectral resolution
of 2 cm–1. All spectral manipulations (water spectrum
subtraction, second derivative, etc.) were performed using the GRAMS/AI
software package.
infrared spectroscopy (FT-IR). Measurements were performed by means
of a Varian 2000 (Scimitar Series) FT-IR spectrometer (Varian Inc.,
US) using a MCT (mercury-cadmium-telluride) detector and fitted with
a “Golden Gate” single reflection diamond ATR accessory
(Specac Ltd., UK). The applied attenuated total reflection (ATR) technique
allows for the study of aqueous solutions. 5 μL of the sample
was pipetted on the top of the diamond ATR element, and the spectrum
was collected immediately with 128 scans at a spectral resolution
of 2 cm–1. All spectral manipulations (water spectrum
subtraction, second derivative, etc.) were performed using the GRAMS/AI
software package.
8
FTIR Spectroscopic Analysis of Dry Film
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A Varian 2000 FTIR Scimitar spectrometer (Varian Inc., Palo Alto,
CA) was used for FTIR spectroscopic measurements. The spectrometer
is fitted with a liquid nitrogen-cooled mercury–cadmium–telluride
(MCT) detector with a “Golden Gate” single reflection
diamond ATR accessory (Specac Ltd., Orpington, U.K.). On the diamond
ATR surface, 5 μL of the sample was mounted and spectrum was
accumulated (2 cm–1 resolution and 64 scans) for
the dry film after gradual evaporation of the buffered solvent under
ambient conditions. ATR correction for every data acquisition, buffer
subtraction, and baseline corrections was performed. The GRAMS/32
software package (Galactic Inc.) was used for all spectral manipulations.
CA) was used for FTIR spectroscopic measurements. The spectrometer
is fitted with a liquid nitrogen-cooled mercury–cadmium–telluride
(MCT) detector with a “Golden Gate” single reflection
diamond ATR accessory (Specac Ltd., Orpington, U.K.). On the diamond
ATR surface, 5 μL of the sample was mounted and spectrum was
accumulated (2 cm–1 resolution and 64 scans) for
the dry film after gradual evaporation of the buffered solvent under
ambient conditions. ATR correction for every data acquisition, buffer
subtraction, and baseline corrections was performed. The GRAMS/32
software package (Galactic Inc.) was used for all spectral manipulations.
9
ATR-FTIR Spectroscopy of Samples
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ATR-FTIR spectra were acquired using a Varian 2000 FTIR Scimitar Series spectrometer fitted with a Golden Gate single reflection diamond ATR accessory (Specac Ltd, UK). 5 μl of sample was mounted on the diamond ATR crystal. The measurements were done after drying the sample by slow evaporation of the solvent buffer under ambient conditions. 64 scans were co-added at a nominal resolution of 2 cm−1. The GRAMS/32 software package (Galactic Inc, USA) was used for all spectral manipulations.
10
Investigating Silica-Amino Group Bonds
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To investigate the bonds between silica particles and amino-groups, ATR-FTIR spectra were acquired using a Varian 2000 FTIR Scimitar Series (Varian, Inc., USA) spectrometer equipped with a liquid nitrogen cooled mercury cadmium telluride (MCT) detector and fitted with a “Golden Gate” single reflection diamond ATR accessory (Specac Ltd., UK). Five microliters of sample was spread on top of the ATR-element and spectra were taken as dry film after gentle evaporation of the solvent. In general 128 scans were summed up at a nominal resolution of 4 cm−1.
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