Pdgfa

Manufactured by Abcam

Sourced in United States

PDGFA is a recombinant protein that represents the platelet-derived growth factor alpha polypeptide chain. It is a member of the platelet-derived growth factor family, which plays a role in cell growth and division.

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Lab products found in correlation

4 protocols using pdgfa

Hypoxia Signaling Pathway Regulation

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Cobalt (II) chloride, DMOG, echinomycin, and etoposide were purchased from Sigma-Aldrich. Daprodustat (GSK1278863) was purchased from DCC Chemicals. VH298 (CAS#2097381-85-4) was purchased from Cayman Chemical. MG was prepared by acid-catalyzed hydrolysis of dimethyl pyruvaldehyde, purified by fractional distillation, and analyzed by NMR as previously described (Tamae et al, 2011 (link)).
Antibodies used include XPA (MA5-13835; Invitrogen), XPC (A301-122A; Bethyl Laboratories), XPD (#11963; CST), XPG (sc12558; SCBT), CSB (24291-AP-1; Proteintech), PHD3 (NB100-139; Novus), HIF-1α (NB100-105 and BD 610959; Novus), HIF-1α-OH P562 (#3434; CST), HIF1AN (MA5-27619; Thermo Fisher Scientific), VEGFA (ab46154; Abcam), PDGFA (ab38562; Abcam), γ-H2AX (NB100-78356; Novus), p-ATR Ser428 (#2853; CST), p-ATM Ser1981 (#13050; CST), ATM (#2873; CST), ATR (#13934; CST), H2AX (#2595; CST), p-AKT (#13038; CST), IDH1 (ab172964; Abcam), α-tubulin-HRP (ab185067; Abcam), GAPDH (sc32233; SCBT), β-actin (#4970, rabbit; CST), β-actin (sc47778, mouse; SCBT), rb-α-ms-HRP (ab6728; Abcam), and gt-α-rb-HRP (ab6721; Abcam).

Ciminera A.K., Shuck S.C, & Termini J. (2021). Elevated glucose increases genomic instability by inhibiting nucleotide excision repair. Life Science Alliance, 4(10), e202101159.

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Antibody Panel for Cellular Signaling

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Antibodies: Histone H3K27Ac (cat. #4353), Histone H3 (cat. #4499), AKT, and p-AKT (cat. #4060), Ki67 (cat. #9129) and ƴH2AX (cat. #9718) were purchased from Cell Signaling Technology (Danvers, MA); p21 (cat. #ab109199) and LANA (cat. #ab4103) from Abcam (Cambridge, MA), PDGFA (cat. #sc-9974), PDGFB (cat. #sc-365805), Cyclin D1 (cat. #sc-718), FLT4/VEGFR3 (cat. #sc321) and p53 (cat. #sc-6243) from Santa Cruz Biotechnology; Actin (cat. #A5441) from Sigma; and p-PDGFRA (Y742) (cat. #AF2114) and total PDGFRA (cat. #AF-307) from R&D Systems (Minneapolis, MN). Anti-CD31 (PECAM1) (cat. #550274) from BD Biosciences. PDGFR tyrosine kinase inhibitor IV (cat. # sc-205794) was purchased from Santa Cruz Biotechnology.

Naipauer J., Rosario S., Gupta S., Premer C., Méndez-Solís O., Schlesinger M., Ponzinibbio V., Jain V., Gay L., Renne R., Chan H.L., Morey L., Salyakina D., Abba M., Williams S., Hare J.M., Goldschmidt-Clermont P.J, & Mesri E.A. (2019). PDGFRA defines the mesenchymal stem cell Kaposi’s sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment. PLoS Pathogens, 15(12), e1008221.

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Protein Expression Analysis by Western Blot

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Protein was isolated from tissue using RIPA buffer. A NanoDrop 2000 (Thermo, USA) was used to measure the protein concentration. The protein samples were separated using SDS‒PAGE. After the protein was transferred to a PVDF membrane, a 3-h incubation in 5% skim milk at room temperature was performed to block nonspecific protein sites. Then, primary antibody was incubated with the membrane overnight. The PVDF membrane was then treated with the secondary antibody. Finally, an ECL luminescence kit (Thermo Scientific, USA) was used to visualize the protein on the PVDF membrane and capture images. Three separate experiments were performed, and the protein expression was analyzed using ImageJ each time. SERPIAN5, OLR1, PDGFA, S100A4 and APOH primary rabbit antibody were purchased from Abcam (Cambridge, USA), MSX1 primary rabbit antibody were purchased from Bioss (Beijing, China), secondary antibody (goat anti-rabbit immunoglobulin) were purchased from Abcam (Cambridge, USA).

Chen B., Han Y., Sheng S., Deng J., Vasquez E., Yau V., Meng M., Sun C., Wang T., Wang Y., Sheng M., Wu T., Wang X., Liu Y., Lin N., Zhang L, & Shao W. (2024). An angiogenesis-associated gene-based signature predicting prognosis and immunotherapy efficacy of head and neck squamous cell carcinoma patients. Journal of Cancer Research and Clinical Oncology, 150(2), 91.

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Comprehensive Protein Expression Analysis

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Samples were lysed with RIPA lysis buffer. Proteins were extracted and subjected to SDS-polyacrylamide gels. Membranes were blocked with 5% skimmed milk and then incubated with primary antibodies, such as USP9X (1:1000), CDK4 (1:1000), CDK6 (1:1500), P21 (1:1000), PCNA (1:1000), Bcl-2 (1:1000), Bax (1:1000), Cleaved caspase 3 (1:1000), ANGPT2 (1:1500), FGF1 (1:1000), PDGFA (1:1000), VEGF (1:2000), CCND1 (1:1000), SOX11 [EPR8192] (1:1000) and β-actin (1:3000) (all from Abcam, Cambridge, MA, USA) at 4°C overnight. The corresponding HRP-conjugated secondary antibody was used to cover membranes. Bands were visualized using enhanced chemiluminescence substrate (Takara, Dalian, China).

Huang G., Liao J., Wang M., Huang Y., Tang M, & Hao Y. (2022). USP9X Increased Tumor Angiogenesis in Mantle Cell Lymphoma by Upregulation of CCND1-Mediated SOX11. Mediterranean Journal of Hematology and Infectious Diseases, 14(1), e2022048.

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