Poly acrylic acid paa

Poly(acrylic acid) (PAA) (M_w = 100,000 Da, 35 wt % in H₂O; data provided by supplier) was obtained from Sigma-Aldrich (Poznań, Poland). Coupling agents (N-Ethyl-N’-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC; 98%) and 4-N,N’-dimethylaminopyridine (DMAP; 98%)) were purchased from Sigma-Aldrich (Poznań, Poland). Fatty alcohols (reagent grade, 98%)—dodecanol and hexadecanol—were obtained from Reachim (Moscow, Russia) and Fluka (St. Gallen, Switzerland), respectively. All other reagents and solvents were purchased from Avantor Performance Materials (Gliwice, Poland). All materials were used without further purification. All experiments and synthetic/purification procedures were performed using deionized water (Millipore).

Tetraethyl orthosilicate (TEOS), poly(allylamine hydrochloride) (PAH, MW≈15,000), and polyacrylic acid (PAA, MW ≈ 1800) were purchased from Sigma-Aldrich (USA). Potassium permanganate (KMnO₄) and sodium carbonate (Na₂CO₃) were obtained from Sinopharm Chemical Reagent CO., Ltd. (China). Coumarin-modified cisplatin and rhodamine-modified docetaxel were purchased from Xian Qiyue Biotechnic CO., Ltd. (China).

Lutetium (III) nitrate hydrate (Lu(NO₃)₃·xH₂O, Sigma-Aldrich, 99.9%, St. Louis, MO, USA), neodymium (III) nitrate hexahydrate (Nd(NO₃)₃·6H₂O, Sigma-Aldrich, 99.9%, St. Louis, MO, USA), sodium orthovanadate (Na₃VO₄, Sigma-Aldrich, 99.9%, St. Louis, MO, USA), ethylene glycol (EG, Sigma-Aldrich, 99.9%, St. Louis, MO, USA), polyacrylic acid (PAA, average Mw~1800, Sigma-Aldrich, St. Louis, MO, USA), 2-morpholinoethanesulfonic acid (MES, Sigma-Aldrich, 99%, St. Louis, MO, USA), and Iohexol (Sigma-Aldrich, analytical standard, ≥95%, St. Louis, MO, USA) were used as received.

All reagents were commercially available and used as received without further purification. Iron (II) sulphate heptahydrate (FeSO₄·7 H₂O), sodium hydroxide (NaOH), potassium nitrate (KNO₃), sulfuric acid (H₂SO₄), poly(ethylenimine) (PEI, M_W = 25 kDa) and poly(acrylic acid) (PAA, Mw = 450 kDa) were obtained from Sigma-Aldrich.

Titanium(IV)
butoxide (TIBU, 97% reagent grade), tetraethyl orthosilicate (TEOS,
98% reagent grade), poly(acrylic acid) (PAA) (M_w = 1800), 1-butanol(anhydrous), and hydrochloric acid (37%
v/v) for the preparation of precursor solutions were purchased from
Sigma-Aldrich. The titania precursor sol is prepared by adding a solution
of TIBU in butanol (1.2 mol/L) to a butanol solution containing PAA
(1 mg/mL) and a catalytic amount of HCl (100 μL) under stirring.
The silica precursor sol is instead prepared by adding a solution
of TEOS in butanol (2.1 mol/L) to a butanol solution containing PAA
(33 mg/mL) and HCl (0.5 mL) under stirring. The solutions are kept
under stirring for at least 4 h before usage. DBRs were fabricated
by alternative dynamic spin coating of the two sols at 11,000 rounds
per minute with short annealing processes (60 s) in between film deposition
at 300 and 500 °C for sSiO₂ and sTiO₂,
respectively.

The materials are as follows: sodium metasilicate (Sigma-Aldrich, St. Louis, MO, USA), highly dispersed pyrogenic silica (A300, Kalush, Ukraine), ACHE solution from electric eel (mw = 280 kDa, Sigma-Aldich), polymeric composition on the base of poly(vinyl alcohol) (PVA) (JVP, Japan) and polyacrylic acid (PAA) (Sigma-Aldrich) (PAA:PVA = 10:1, pH = 6) synthesized in regard to [7 ], 0.067 M solution of Na, K-phosphate buffer (pH 6), 1 M solution of hydrochloric acid (Sigma-Aldrich), sodium chloride (Sigma-Aldrich), sodium hydroxide (Sigma-Aldrich), magnesium chloride hexahydrate (Sigma-Aldrich), acetylcholine chloride (Sigma-Aldrich), and CHE from the liver homogenate of G. gallus which was obtained as shown elsewhere [8 ].