Vitreous and retinae from pups were collected and rapidly frozen on dry ice. To detect dopamine levels, vitreal samples were pooled from 3–6 pups depending on age and 6 retinae for each “n”. Dopamine extraction and ELISA assay were performed according to manufacture protocol using BA E-5300 (Rocky Mountain Diagnostics). For DD experiments, vitreous and retinae were collected under dim red light. To detect VEGFA and FLT1 levels, samples from P5 pups were pooled from 6 eyes for each “n”. Mouse VEGFA kit, Quantikine (MMV00) and mouse VEGFR1 (FLT1), Quantikine (MVR100) from R&D systems were used. ELISA was read by using EnVision Multimode Plate Reader (Perkin Elmer).
Quantikine mvr100
Manufactured by R&D Systems
The Quantikine (MVR100) is a solid-phase enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of human Microvascular Endothelial Cell Protein C Receptor (PROCR) in cell culture supernates, serum, and plasma.
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Lab products found in correlation
3 protocols using quantikine mvr100
1
Quantifying Retinal and Vitreous Molecular Markers
2
Quantification of mouse Flt1 protein
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Quantification of mouse Flt1 protein levels was performed according to the manufacturer's instructions (R&D Systems, Quantikine MVR100). Briefly, MN explants of two E11.5 SCs were cultured in 250 μl starvation medium, either in absence or presence of 10 U ml−1 heparin (H4784, Sigma-Aldrich) for 20 h. Total cellular protein of MN explants was extracted using ELISA-extraction buffer. MN-CM was directly subjected to the assay.
3
Quantifying Retinal and Vitreous Molecular Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Vitreous and retinae from pups were collected and rapidly frozen on dry ice. To detect dopamine levels, vitreal samples were pooled from 3–6 pups depending on age and 6 retinae for each “n”. Dopamine extraction and ELISA assay were performed according to manufacture protocol using BA E-5300 (Rocky Mountain Diagnostics). For DD experiments, vitreous and retinae were collected under dim red light. To detect VEGFA and FLT1 levels, samples from P5 pups were pooled from 6 eyes for each “n”. Mouse VEGFA kit, Quantikine (MMV00) and mouse VEGFR1 (FLT1), Quantikine (MVR100) from R&D systems were used. ELISA was read by using EnVision Multimode Plate Reader (Perkin Elmer).
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