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Milliplex map human rank l magnetic bead

Manufactured by Merck Group

The MILLIPLEX MAP Human RANK-L Magnetic Bead is a lab equipment product designed for the quantitative detection of human receptor activator of nuclear factor kappa-B ligand (RANK-L) in biological samples. It utilizes magnetic beads coated with specific antibodies to capture and measure the target analyte.

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2 protocols using milliplex map human rank l magnetic bead

1

Measurement of Inflammatory Biomarkers in Tissue

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Freshly obtained tissue specimens were prepared as previously described.5 (link) The concentration of ECP, RANK-L, IL-5 and IL-13 in cell-free supernatants was measured by ELISA kit (MBL, Woburn, Mass), and Luminex multiplex assay (MILLIPLEX MAP Human RANK-L Magnetic Bead and MILLIPLEX MAP Human Cytokine/Chemokine Panel from EMD Millipore, Burlington, Mass). The minimal detection limits for ECP, RANK-L, IL-5 and IL-13 are 0.125 ng/ml, 5.0 pg/ml, 3.2 pg/ml and 3.2 pg/ml, respectively. The concentration of RANK-L, IL-13 and ECP in tissue homogenates was normalized to the concentration of total protein as detected by BCA protein assay kit (Thermo Fisher Scientific Inc., Waltham, Mass).
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2

Measurement of Inflammatory Biomarkers in Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freshly obtained tissue specimens were prepared as previously described.5 (link) The concentration of ECP, RANK-L, IL-5 and IL-13 in cell-free supernatants was measured by ELISA kit (MBL, Woburn, Mass), and Luminex multiplex assay (MILLIPLEX MAP Human RANK-L Magnetic Bead and MILLIPLEX MAP Human Cytokine/Chemokine Panel from EMD Millipore, Burlington, Mass). The minimal detection limits for ECP, RANK-L, IL-5 and IL-13 are 0.125 ng/ml, 5.0 pg/ml, 3.2 pg/ml and 3.2 pg/ml, respectively. The concentration of RANK-L, IL-13 and ECP in tissue homogenates was normalized to the concentration of total protein as detected by BCA protein assay kit (Thermo Fisher Scientific Inc., Waltham, Mass).
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