Measurement of lean tissue, fat and fluid in living mice was performed using Bruker’s minispec Body Composition Analyzer. Plasma samples and livers were collected from ad-lib fed mice for triglyceride and cholesterol measurements. Total cholesterol and triglyceride was measured using standard enzymatic assays by the Vanderbilt University Medical Center Lipid Core.
Minispec body composition analyzer
Manufactured by Bruker
The Minispec Body Composition Analyzer is a laboratory instrument designed to measure body composition. It uses nuclear magnetic resonance (NMR) technology to determine the amount of fat, lean tissue, and water in a sample.
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Lab products found in correlation
D12450bi, by Research Diets (1 mentions)
Rapamycin, by LC Laboratories (1 mentions)
D12492, by Research Diets (1 mentions)
D12330, by Research Diets (1 mentions)
Echomri 100, by Echo Medical Systems (1 mentions)
D12328, by Research Diets (1 mentions)
Lunar piximus2 mouse densitometer, by GE Healthcare (1 mentions)
B6 cg lepob j, by Jackson ImmunoResearch (1 mentions)
9 protocols using minispec body composition analyzer
1
Measurement of Body Composition in Mice
2
Dietary Fat Induced Metabolic Changes
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Mice were placed on NCD (10% kcal fat; Research Diets D12450Bi) or HFD (45% kcal fat; Research Diets D12451i) starting at 4–6 weeks of age for 12 to 18 weeks. Mice were weighed weekly, and food consumption was tracked. Body composition, including body fat, lean mass, and fluid, was measured via TD-NMR using the Bruker Minispec Body Composition Analyzer. Energy expenditure, movement, VO2 max, VCO2 max, respiratory exchange ratio (RER), and food and drink consumption were measured using CLAMS metabolic cages, which was performed by the Metabolic Phenotyping Core. Serum was collected from mice treated with HFD for 0, 2 or 17 weeks and Leptin levels were measured via MAGPIX, using the mouse metabolic hormone panel (Millipore). For the rapamycin experiments, mice were placed on HFD for four weeks prior to administration of rapamycin, and then kept on HFD and intraperitoneally (i.p.) injected with rapamycin (LC Laboratories) three times per week for 10 weeks. rapamycin was prepared in a solution of 100% ethanol, 10% PEG400, and 10% Tween80 and was injected at 4 mg/kg based on body weight.
3
Characterization of Pde9a-deficient Mice
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Animal studies were conducted at Sanford Burnham Prebys Medical Discovery Institute (SBP), Vanderbilt University Medical Center (VUMC), and Johns Hopkins University and School of Medicine (JHSOM). Pde9a−/− mice on a C57BL/6J background were previously described (37 (link)). Mice were maintained on a 12-h light/dark cycle with two to five animals per cage and ad libitum food and water. At 6 weeks of age, SBP and VUMC mice received nutrient-matched Surwit diets: a control low-fat diet (LFD) (10.5% calories from fat: D12328; Research Diets) or high-fat diet (HFD) (58.0% calories from fat: D12330; Research Diets). JHSOM mice were fed HFD (60.0% calories from fat: D12492; Research Diets). Mice were weighed twice weekly, and body composition was measured using a Minispec Body Composition Analyzer (Bruker) at SBP and Vanderbilt Mouse Metabolic Phenotyping Center (VMMPC) or EchoMRI-100 (Echo Medical Systems) at JHSOM. Mice were fasted for 5-h prior to euthanasia by CO2 asphyxiation and exsanguination via cardiac puncture. All procedures were approved by the Institutional Animal Care and Use Committees at SBP, VUMC, and JHSOM.
4
Body Composition Analysis in Mice
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Bruker's minispec Body Composition Analyzer was used to determine the body composition of mice based on Time Domain NMR (TD-NMR). This equipment acquires and analyzes TD-NMR signals from all protons in the entire sample volume and provides a precise method for measurement of lean tissue, fat, and free body fluid in living mice. Body composition was analyzed in mice between 14 and 19 weeks of age. Nineteen male mice and 10 female mice were utilized in these experiments.
5
Metabolic Profiling of C57BL/6J Mice
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Twelve-week old male C57BL/6J mice (n = 4 for each group) were weighed, divided into groups, and allowed to entrain to a LD 12 : 12 cycle for one week. After entrainment, mice were individually housed in Sable Systems respiratory chambers at Vanderbilt University's Mouse Metabolic Phenotyping Center (MMPC) to monitor the rates of VCO2 and VO2 at 5-min intervals which were used to calculate the RER (as VCO2/VO2). EE was also calculated from the VCO2 and VO2 data using the Weir heat equation. CO and LO rates were calculated using equations described by Frayn, Hall and co-workers [34 (link),35 (link)]. Mice were weighed weekly. HFD and RC were placed in automated feeders that recorded the quantity and time of chow retrieval. Mouse activity was monitored via infrared beam detectors. Body composition was determined for mice during day 6 and day 10 of feeding regimens using a Bruker Minispec Body Composition Analyzer.
6
Body Composition and Skeletal Analysis
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Fat mass was quantified using echoMRI-based MiniSpec Body Composition Analyzer from Bruker. Hindlimb bone mineral density was quantified using Dual-Energy X-ray Absorptiometry (DEXA) (Lunar PIXImus 2 Mouse Densitometer equipment, GE Medical Systems). The same equipment was also used to obtain images for the quantification of thoracic-lumbar curvature. Thymi were weighted after fixation in 10% histological formalin (~48 h). Spleens were fixed similarly and weighted after transfer into 70% EtOH.
7
Comprehensive Body Composition Analysis
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Measurement of lean tissue, fat, and fluid in living mice was performed using Bruker’s minispec Body Composition Analyzer. Plasma samples and livers were collected from ad lib fed mice for triglyceride and cholesterol measurements. Total cholesterol and triglycerides were measured using standard enzymatic assays by the Vanderbilt University Medical Center Lipid Core.
8
Dietary Interventions in Obesity Models
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These were started immediately in ob/ob [B6.Cg-Lepob/J; Jax Stock #00632, the Jackson laboratory (Bar Harbor, ME)] or C57bl/6J (to induce DIO) after weaning (age of 4 to 5 weeks), with an intent to change visceral fat composition. The diets are detailed in fig. S4 and were given ad libitum. Normal chow [Purina 5053 diet, LabDiet (www.labsupplytx.com/wp-content/uploads/2012/10/5053.pdf ), which contains 5.0% of which ≈70% is UFA] and water were fed to control mice ad libitum. Mice were weighed periodically (1 to 4 weeks, depending on the rate of weight gain), and the body fat was measured using a Minispec Body Composition analyzer (Bruker Corporation, Billerica, MA) using nuclear magnetic resonance as previously described (27 (link)).
9
Noninvasive Mouse Body Composition
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Body fat and lean tissue were measured using Mini-Spec Body Composition Analyzer (Bruker). Unanesthetized mice were placed in a clear plastic tube and gently restrained at the end of the tube by using a plunger with air holes to minimize movement. The tube was then inserted into the mini-spec port to a premeasured depth and the measurements were collected.
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