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5 protocols using rhamnose rha

1

Codonopsis pilosula Root Polysaccharide Extraction

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The dried roots of Codonopsis pilosula were collected from Livzon Pharmaceutical Grouping Limin Pharmaceutical Factory. (P.R. China). Standard monosaccharides including Ara, Gal, Glc, Man, rhamnose (Rha), xylose (Xyl), glucuronic acid (GlcA), and galacturonic acid (GlaA) were purchased from Sigma (Louis, MO, USA). DEAE-52 cellulose was purchased from Whatman (Kent, UK). 1-Pheny-3-methyl-5-pyrazolone (PMP), dextrans (2,000,000, 300,600, 135,350, 64,650, 36,800, 13,050, 9750, 5250, 2700, and 180 Da) were purchased from the national institutes for food and drug control (Beijing, China). All other chemicals were of analytical grade.
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2

Evaluation of Yam Bulbils and Rhodiola

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Yam bulbils were purchased from Jianguo Pharmaceutical Co. Ltd. (Jiaozuo, Henan, China). Commercially available large plant Rhodiola capsules (LPRC), employed as the positive control group (PG), were purchased from Kanion Pharmaceutical Co. Ltd. (Jiangsu, China). Assay kits for evaluating biological indicators (blood lactic acid, BLA; malondialdehyde, MDA; blood urea nitrogen, BUN; hepatic glycogen; superoxide dismutase, SOD; and glutathione peroxidase, GSH‐Px) were purchased from Nanjing Jiancheng Biotechnology Institute (Nanjing, Jiangsu, China). Standard sugars (rhamnose, Rha; glucose, Glu; mannose, Man; galactose, Gal; arabinose, Ara; and xylose, Xyl), were purchased from Sigma‐Aldrich (St Louis, MO, USA). Other reagents were of analytical grade.
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3

Extraction and Characterization of L. japonica Compounds

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L. japonica was purchased from Xiapu County, Fujian Province, China. Deuterium oxide (D2O, 99.9% atom D), 3-Methyl-1-phenyl-2-pyrazolin-5-one (PMP), glucose (Glc), GlcA, Gal, galacturonic acid (GalA), Xyl, and rhamnose (Rha) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Bovine serum albumin (BSA), trifluoroacetic acid (TFA), heparin, Fuc, ribose (Rib), arabinose (Ara), and Man were purchased from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). DEAE-Sepharose FF was from GE Healthcare (Uppsala, Sweden). CaCl2 solution (0.05 M), TT, PT, and APTT assay kits, and coagulation control plasma were obtained from TICO GmbH (Hamburg, Germany). Biophen FVIII: C kit was purchased from Hyphen Biomed (Neuvillesur-Oise, France). LMWH (0.4 mL × 4000 AXaIU) was from Sanofi-Aventis (Paris, France). Human factor VIII was obtained from Bayer Healthcare LLC (Leverkusen, Germany). All other chemicals, such as HCl, NaOH, and NaCl, were of analytical grade.
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4

Extraction and Characterization of Mangrove Fruit Polysaccharides

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A. marina (Forssk.) Vierh. fruits were collected from Beihai (Guangxi, China). Monosaccharide standards, such as mannose (Man), ribose (Rib), arabinose (Ara), and fucose (Fuc), and bovine serum albumin (BSA), were obtained from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). 3, 5-Dinitrosalicylic acid (DNS) was obtained from Shanghai Solarbio Bioscience & Technology Co., Ltd (Shanghai, China). Lipase (Lot No. SLBL2143V, 41.62 ± 5.62 U/mg determined using triacetin), α-amylase (Lot No. BCCB0496, 30.80 ± 6.32 U/mg), pancreatin (Lot No. SLBV6830, activities of Lipase, amylase, protease in pancreatin were 59.844 ± 10.18, 116.10 ± 12.73, and 135.93 ± 2.11 U/mg, respectively), pepsin (Lot No. BCBR2017V, 301.40 ± 23.98 U/mg), 3-methyl-1-phenyl-2-pyrazolin-5-one (PMP), glucose (Glc), galactose (Gal), galacturonic acid (GalA), glucuronic acid (GlcA), and rhamnose (Rha) were provided by Sigma Chemical Co. (St. Louis, MO, USA). Coomassie brilliant blue G-250, fructooligosaccharide (FOS), trypsin (Lot No. M54318066, 357.35 ± 5.53 U/mL), xylose (Xyl), and amyloglucosidase from Aspergillus niger (Lot No. C10778757, > 100,000 U/mL) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Other commercially obtained chemicals, such as NaCl, NaHCO3 and NaOH, were of analytical grade.
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5

Sugar Profiling of Sugarcane Honey

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SCH samples were provided by the certified producer Fábrica de Mel-de Cana do Ribeiro Sêco in April 2016 (FRS16), 2017 (FRS17), and 2018 (FRS18) (Madeira Island, Portugal), and stored under stable conditions (4 °C, in the dark). Sugars standards, glucose (GLU), fructose (FRU), sucrose (SUC), xylose (XYL), mannose (MAN), and rhamnose (RHA) were purchased from Sigma-Aldrich (St. Louis, USA). Acetone (ACT), acetonitrile (ACN), ethanol (EtOH), methanol (MeOH), and triethylamine (TEA) were purchased from Sigma-Aldrich (St. Louis, USA). Ultrapure deionized water (H 2 O), purified with a Milli-Q ultra-pure water system from Millipore (Massachusetts, USA). All solvents and samples were filtered through 0.22 μm membrane filters from Millipore (Massachusetts, USA), before analysis.
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