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Nadph

Manufactured by Abcam
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NADPH is a coenzyme that plays a crucial role in numerous cellular processes. It serves as a reducing agent, donating electrons to various enzymatic reactions. NADPH is essential for anabolic pathways, such as lipid and nucleotide synthesis, and is also involved in maintaining the balance of cellular redox states.

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Lab products found in correlation

Genejet plasmid miniprep kit, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by New England Biolabs (1 mentions) Bspqi, by New England Biolabs (1 mentions) Na2seo3, by Thermo Fisher Scientific (1 mentions) Coomassie plus bradford reagent, by Thermo Fisher Scientific (1 mentions) Pcr cleanup kit, by Thermo Fisher Scientific (1 mentions) T4 ligase, by New England Biolabs (1 mentions) Q5 high fidelity polymerase, by New England Biolabs (1 mentions) E coli bl21 de3, by New England Biolabs (1 mentions) Dntps, by New England Biolabs (1 mentions)

Close spelling variants of this product

NADP/NADPH fluorometric assay kit Colorimetric NADP/NADPH assay kit NADPH quantification kit NADP+/NADPH extraction buffer NADP/NADPH Quanti cation Colorimeteric Kit Amplite TM Colorimetric NADP/NADPH Ratio Assay Kit NADP/NADPH determination kit NADPH Assay Kit NADPH standard NADPH Quantitation Fluorometric Assay Kit

2 protocols using nadph

1

Phage Display Kit Protocol

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The Ph.D.™ Phage Display Kit, BSA, BspQI, T4 Ligase, Q5 High Fidelity polymerase, dNTPs, and BL21(DE3) E. coli were purchased from New England Biolabs. Antibiotics were purchased from GoldBio. Na2SeO3 and HNaSeO3 were purchased from Alfa Aesar. NADPH was purchased from BioVision and Coomassie Plus Bradford Reagent from Thermo Scientific. GeneJet Plasmid Miniprep Kit (Cat# K0503) and PCR Cleanup Kit (Cat# K0702) were purchased from ThermoFisher Scientific.
Butz Z.J., Borgognoni K., Nemeth R., Nilsson Z.N, & Ackerson C.J. (2020). Metalloid Reductase Activity Modified by a Fused Se0 Binding Peptide.. ACS chemical biology, 15(7), 1987-1995.
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2

Quantifying NADPH and α-KG Levels

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HeLa cells expressing control shRNA or indicated shRNAs, psin, or psin-IDH1-AS1 were cultured. NADPH or α-KG levels in the cell lysates were determined using a fluorescence-based lactate assay kit (AAT Bioques for NADPH, BioVision for α-KG) according to the manufacturers’ instructions.
Xiang S., Gu H., Jin L., Thorne R.F., Zhang X.D, & Wu M. (2018). LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect. Proceedings of the National Academy of Sciences of the United States of America, 115(7), E1465-E1474.
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