Human total mmp 7 quantikine elisa kit

There were three ways to obtain MMP-7 serum samples in the current study: (1) No transportation required (blood sample from the Wuhan Union Hospital): Blood samples from 224 subjects were collected in a coagulation-promoting tube and placed in a 4℃ refrigerator. (2) Transportation time less than 12 hours (other two hospitals in Wuhan): the blood samples from 83 subjects were gathered, stored in an ice box (≤ 4°C) and transported to Wuhan Union Hospital for tests. For the above two situations, the blood samples were tested directly after centrifugation (4000 rpm/5 min) at 4°C. (3) Transportation time greater than 12 hours: serum samples from 133 subjects were packed in a thermally isolated box lled with dry ice and transported to Wuhan Union Hospital. Samples were tested directly after they were received.
According to the protocol, serum and stool MMP-7 levels were measured using a Human Total MMP-7
Quantikine ELISA Kit (R&D, USA). The dilution times were 8 and 32 for serum samples. Stool samples were washed with PBS three times immediately after collection. The nal concentration of stool was 1 g to 9 ml PBS, and the sample was subjected to ultrasound for 30 seconds. The stool solution was centrifuged for 5 minutes at 5000 g, and the supernatant was used for ELISA tests.

The concentrations of TNF-α, IL1β, IL6, IL1Ra, and CCL18 were measured in macrophage cell culture supernatants using human DuoSet ELISA kits (R&D Systems) according to manufacturer instructions. Concentration of MMP-7 in supernatants was measured by human Total MMP-7
Quantikine ELISA Kit (R&D systems).