Portable glucometer

Manufactured by Bayer

Sourced in United States

The Portable Glucometer is a compact, handheld device designed to measure and display the user's blood glucose level. It provides a quick and convenient way to monitor blood sugar levels for individuals with diabetes or those who require regular glucose monitoring.

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Lab products found in correlation

Blood glucose test strips, by Bayer (1 mentions) Nod scid mice, by Jackson ImmunoResearch (1 mentions) Streptozotocin (stz), by Merck Group (1 mentions) C57bl 6ncrl female mice, by Charles River Laboratories (1 mentions)

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Glucometer (106 citations)

5 protocols using portable glucometer

Renal Histopathology and Biomarker Analysis

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The fasting blood glucose level was detected using a portable glucometer (BAYER, Germany) with blood glucose test strips (BAYER, Germany). The serum and urine samples were thawed at 4 ℃ before detection. Serum cystosin C (Cys-C) levels were measured with a mouse Cys-C ELISA kit (E-EL-M3024, Elabscience, Wuhan, China). Urine creatinine (UCR) and urine microalbuminuria albumin levels were measured using a UCR enzyme-linked immunoassay kit (MM-44289M1, Elabscience, Wuhan, China) and MAU/ALB enzyme-linked immunoassay kit (MM-0705M1, Elabscience, Wuhan, China), respectively.
For renal histopathological analysis, paraffin-embedded renal tissues were cut into 4-um sections and subjected to haematoxylin–eosin (H&E), Masson and PAS staining. Subsequently, 10 randomly selected non-overlapping areas (25 × 25 μm) on HE-stained images (200×) were used to evaluate the extent of glomerular damage by calculating the average glomerular perimeter and area using the ImageJ software. The same method was used to select 10 fields of view on Masson-stained images and determine the percentage of the fibrotic area (blue-stained area) in each observed field for assessing the degree of glomerular and interstitial fibrosis. Renal histopathological analysis was performed by two independent investigators using a blinded method.

Lu Y.P., Zhang Z.Y., Wu H.W., Fang L.J., Hu B., Tang C., Zhang Y.Q., Yin L., Tang D.E., Zheng Z.H., Zhu T, & Dai Y. (2022). SGLT2 inhibitors improve kidney function and morphology by regulating renal metabolic reprogramming in mice with diabetic kidney disease. Journal of Translational Medicine, 20, 420.

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Islet Cell Transplantation in Diabetic Mice

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NOD SCID mice purchased from the Jackson Laboratory (Bar Harbor, MA, USA) were used as transplant recipients. Diabetes was induced by single intraperitoneal injection of 150 mg/kg streptozotocin (Sigma-Aldrich Corp and blood glucose was monitored daily via tail-nick blood drops on a portable glucometer (Bayer, Burr Ridge, IL, USA). Mice were considered hyperglycemic after 3 consecutive days with blood glucose readings >350 mg/dL. On the day of transplantation, donor islets were washed and resuspended in aliquots of Connaught Medical Research Laboratories (CMRL) transplant media (Mediatech, Inc.). Diabetic mice were anesthetized by isoflurane inhalation, and islets were implanted underneath the renal capsule as described elsewhere.^{27 (link)} Blood glucose was monitored for 30 d after transplantation. Graft-bearing kidneys were then removed from recipients who achieved normoglycemia, and blood glucose was monitored for restoration to hyperglycemia within 72 h.

Danobeitia J.S., Chlebeck P.J., Shokolenko I., Ma X., Wilson G, & Fernandez L.A. (2018). Novel Fusion Protein Targeting Mitochondrial DNA Improves Pancreatic Islet Functional Potency and Islet Transplantation Outcomes. Cell Transplantation, 26(11), 1742-1754.

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Glucose and Pyruvate Tolerance Tests

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For oral glucose tolerance test (OGTT), mice were fasted 12 h, followed by gavage with glucose (2 g/kg of body weight). For pyruvate tolerance test (PTT), 6 h fasted mice received an intraperitoneal sodium pyruvate (2 g/kg of body weight) injection. Blood samples of mice were collected from the tail vein at 0, 15, 30, 45, 60, 90 and 120 min after glucose or pyruvate administration, and determined using a portable glucometer (Bayer, German).

Li J., Huang Y., Yang X., Cai Y., Wang Y., Dai W., Jiang L., Wang C, & Wen Z. (2024). Tyrosine-phosphorylated DNER sensitizes insulin signaling in hepatic gluconeogenesis by inducing proteasomal degradation of TRB3. Molecular Metabolism, 83, 101927.

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Glucose Tolerance Test in Mice

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Targeted mice aged 10–23 weeks were fasted for 14 h, followed by glucose injection (2 g/kg body weight via intraperitoneal injection). Venous blood was drawn from the tail vein at 0 (just before the injection), 15, 30, 60, 90 and 120 min after the injection of glucose. Blood glucose level was measured using a portable glucometer (Bayer, Elkhart, IN) as described previously [25 (link)].

Wang L., Sun Z.S., Xiang B., Wei C.J., Wang Y., Sun K., Chen G., Lan M.S., Carmona G.N., Notkins A.L, & Cai T. (2018). Targeted deletion of Insm2 in mice result in reduced insulin secretion and glucose intolerance. Journal of Translational Medicine, 16, 297.

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Combination Therapy for Mammary Cancer

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Female C57BL/6NCrl mice (n = 120) were purchased from Charles River (Wilmington, MA, USA) and allowed to acclimate to a low-fat control diet (10 kcal% from fat, Research Diets D12450J) ad libitum. At 36 weeks of age, mice were orthotopically injected into the fourth mammary fat pad with 50,000 metM-Wnt^lung mammary cancer cells in 50 μL sterile phosphate-buffered saline. Tumors grew until reaching a mean size of 100 mm³, at which time mice were block randomized to receive either vehicle, BMS-754807 (6.25 mg/kg), HCQ (60 mg/kg), CP (50 mg/kg), or combinations of two or three drugs. Doses used for in vivo analysis were based on the previous literature [31 (link),32 (link),33 (link),34 (link),35 (link),36 (link)]. BMS-754807 and/or HCQ were delivered by daily intraperitoneal injection in a vehicle of 30% PEG300, 5% DMSO, 5% Tween80, and 60% water. CP dissolved in water was delivered by once-weekly intraperitoneal injection. After 23 d of drug treatment (1–2 d prior to euthanasia), blood glucose was assessed via tail nick and portable glucometer (Bayer, Pittsburgh, PA, USA). Blood glucose assessment was performed 2 h and 6 h after drug treatment. Tumor sizes were monitored three times a week until average tumor size in the largest treatment group reached a volume of 1250 mm³, at which time all mice were euthanized, and tumors were excised and weighed.

Ho A.N., Kiesel V.A., Gates C.E., Brosnan B.H., Connelly S.P., Glenny E.M., Cozzo A.J., Hursting S.D, & Coleman M.F. (2024). Exogenous Metabolic Modulators Improve Response to Carboplatin in Triple-Negative Breast Cancer. Cells, 13(10), 806.

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