For immunofluorescence staining, primary mouse anti-human ZO-1 (1:100, clone #ZO1–1A12, lot #Q1215680, Cat #33–9100) was from Thermo Fisher Scientific. Secondary donkey anti-mouse IgG conjugated with Alexa Fluor 488 (1:500, lot #1741782, Cat #A-21202) was from Life Technologies. For western blot, primary polyclonal rabbit anti-VHL antibody (1:100, lot #RD2197039, Cat #PA5–27322) was from Thermo Fisher Scientific. Mouse anti-β-actin (1:5,000, clone #C4, lot #I1018, Santa Cruz, Cat #sc-47778) was used as loading control. IRDye 680RD goat anti-rabbit (1: 20,000, lot #C70406–04, Cat #LI-COR926–68171, LI-COR Biosciences) and IRDye 800CW goat-anti-mouse (1: 20,000, lot #C70310–02, Cat #LI-COR827–08364, LI-COR Biosciences) were used as secondary antibodies.
Secondary donkey anti mouse igg conjugated with alexa fluor 488
Manufactured by Thermo Fisher Scientific
The Secondary Donkey Anti-Mouse IgG Conjugated with Alexa Fluor 488 is a fluorescently labeled secondary antibody. It is designed to detect and bind to mouse primary antibodies, allowing for the visualization and detection of target proteins or molecules in various analytical and research applications.
Automatically generated - may contain errors
2 protocols using secondary donkey anti mouse igg conjugated with alexa fluor 488
1
Immunofluorescence and Western Blot Analysis
2
Immunofluorescence and Western Blot Analysis
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For immunofluorescence staining, primary mouse anti-human ZO-1 (1:100, clone #ZO1–1A12, lot #Q1215680, Cat #33–9100) was from Thermo Fisher Scientific. Secondary donkey anti-mouse IgG conjugated with Alexa Fluor 488 (1:500, lot #1741782, Cat #A-21202) was from Life Technologies. For western blot, primary polyclonal rabbit anti-VHL antibody (1:100, lot #RD2197039, Cat #PA5–27322) was from Thermo Fisher Scientific. Mouse anti-β-actin (1:5,000, clone #C4, lot #I1018, Santa Cruz, Cat #sc-47778) was used as loading control. IRDye 680RD goat anti-rabbit (1: 20,000, lot #C70406–04, Cat #LI-COR926–68171, LI-COR Biosciences) and IRDye 800CW goat-anti-mouse (1: 20,000, lot #C70310–02, Cat #LI-COR827–08364, LI-COR Biosciences) were used as secondary antibodies.
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