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Trivalent antimony

Manufactured by Merck Group
Sourced in United States, Belgium

Trivalent antimony is an inorganic compound that consists of antimony in its trivalent oxidation state. It is a colorless, crystalline solid. Trivalent antimony is a laboratory reagent used in various chemical applications and analyses.

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4 protocols using trivalent antimony

1

Antimony-Based Leishmania Treatment Assay

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Trivalent antimony (SbIII), amphotericin B (AmB), paromomycin, Triton X-100, paraformaldehyde, 4′,6-diamidino-2-phenylindole dilactate (DAPI), n-dodecyl-β-D-maltoside (DDM), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and phorbol 12-myristate 13-acetate (PMA) were obtained from Sigma-Aldrich (St. Louis, USA). Miltefosine was purchased from Æterna Zentaris (Frankfurt, Germany). Glucantime® was purchased from Sanofi-Aventis (Paris, France). L-glutamine and penicillin/streptomycin were obtained from Gibco. All chemicals were of the highest quality available.
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2

Antiparasitic Compound Screening Protocol

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Amphotericin B (AmB), trivalent
antimony (SbIII), paromomycin (PMM), Triton X-100, 4′,6-diamidino-2-phenylindole
dilactate (DAPI), phorbol 12-myristate 13-acetate (PMA), resazurine,
and sodium dodecyl sulfate (SDS) were purchased from Sigma-Aldrich
(St. Louis, MO). Miltefosine (Mil) was purchased from Zentaris GmbH
(Frankfurt, Germany). l-Glutamine and penicillin/streptomycin
were obtained from Gibco. All chemicals were of the highest quality
available.
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3

Bioactive Compound Screening for Leishmaniasis

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M199 medium, DMEM-medium, RPMI-1640, MEM non-essential amino acids, L-glutamine, penicillin-streptomycin (P/S) solution, fetal bovine serum (FBS), sodium pyruvate, phosphate buffered saline (PBS) and hemin were purchased from Invitrogen (Ghent, Belgium). Phorbol 12-myristate 13-acetate (PMA), trivalent antimony (SbIII: potassium antimonyl tartrate), EDTA, HEPES, potato starch, Giemsa, adenine, paromomycin (PMM) and Histopaque-1077 were purchased from Sigma Aldrich (Diegem, Belgium). Miltefosine (MIL) was available from Carbosynth (Berkshire, UK), amphotericin B deoxycholate (AmB: Fungizone Bristol-Myers Squibb (Brussels, Belgium), sodium-stibogluconate (SbV; SSG) from Calbiochem (San Diego, US) and 6-biopterine from Schrickx Labs (Bauma, Switzerland).
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4

Antimony and Antifungal Compounds Evaluation

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Trivalent antimony (SbIII), paromomycin (PMM), amphotericin B (AmB), Triton X-100, paraformaldehyde, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], rhodamine 123 (Rh123), phorbol 12-myristate 13-acetate (PMA), Trizol (TRI) Reagent, were obtained from Sigma-Aldrich (St. Louis, MO). Miltefosine (MIL) was purchased from Zentaris GmbH (Frankfurt am Main, Germany). Hygromycin B (HyB), CellTracker, dichlorodihydrofluorescein diacetate (H2DCFDA), MitoSOX red, and 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) were purchased from Invitrogen (Carlsbad, CA). Geneticin G418 Sulphate, L-glutamine, and penicillin/streptomycin were obtained from Gibco. Nourseothricin was obtained from Werner Biogents. All chemicals were of the highest quality available.
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