Whole-cell lysates and total exosomal proteins were prepared by using RIPA buffer (50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate). 50ug total proteins were electrophoretically separated on 4–12% SDS-acrylamide Gel (Thermo Fisher Scientific). Akt3 and PI3K p110α were examined in the study. Western blot analyses were performed with primary antibodies: anti- β-actin, anti-Akt 3, anti-P-Akt, anti-PI3K, anti-VEGFA (1: 1000, Cell Signaling Technology, USA), and the corresponding secondary antibodies anti-mouse and anti-rabbit peroxidase-linked (1: 10 000; Cell Signaling Technology, USA). The signals were visualized by ECL Prime Western Blotting Detection Reagent (Advansta, USA).
Anti mouse and anti rabbit peroxidase linked
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-mouse and anti-rabbit peroxidase-linked secondary antibodies are detection reagents used in western blotting, ELISA, and other immunoassay techniques. They are designed to bind to primary antibodies raised in mouse or rabbit, and the conjugated peroxidase enzyme can then be used to generate a colorimetric or chemiluminescent signal for detection and quantification of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Anti p akt, by Cell Signaling Technology (3 mentions)
Anti β actin, by Cell Signaling Technology (3 mentions)
Ecl prime western blotting detection reagent, by Advansta (3 mentions)
Anti akt3, by Cell Signaling Technology (3 mentions)
Anti pi3k, by Cell Signaling Technology (3 mentions)
Anti vegfa, by Cell Signaling Technology (3 mentions)
3 protocols using anti mouse and anti rabbit peroxidase linked
1
Western Blot Analysis of Akt3 and PI3K
2
Protein Expression Analysis in Exosomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole-cell lysates and total exosomal proteins were prepared by using RIPA buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate). 50ug total proteins were electrophoretically separated on 4-12% SDS-acrylamide Gel (Thermo Fisher Scientific). Akt3 and PI3K p110α were examined in the study. Western blot analyses were performed with primary antibodies: anti-β-actin, anti-Akt 3, anti-P-Akt, anti-PI3K, anti-VEGFA (1: 1000, Cell Signaling Technology, USA), and the corresponding secondary antibodies anti-mouse and anti-rabbit peroxidase-linked (1: 10 000; Cell Signaling Technology, USA). The signals were visualized by ECL Prime Western Blotting Detection Reagent (Advansta, USA).
3
Western Blot Analysis of Akt3 and PI3K
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole-cell lysates and total exosomal proteins were prepared by using RIPA buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate). 50ug total proteins were electrophoretically separated on 4-12% SDS-acrylamide Gel (Thermo Fisher Scienti c). Akt3 and PI3K p110α were examined in the study. Western blot analyses were performed with primary antibodies: anti-β-actin, anti-Akt 3, anti-P-Akt, anti-PI3K, anti-VEGFA (1: 1000, Cell Signaling Technology, USA), and the corresponding secondary antibodies anti-mouse and anti-rabbit peroxidase-linked (1: 10 000; Cell Signaling Technology, USA). The signals were visualized by ECL Prime Western Blotting Detection Reagent (Advansta, USA).
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