Whole, marrow flushed femora were collected and homogenised using the Omni tissue homogeniser (Omni, Kennesaw, GA, USA) in Trizol reagent (Ambion, Austin, TX, USA). RNA was extracted using the NucleoSpin RNA Plus extraction kit (Macherey-Nagel, Düren, Germany). RNA quality was confirmed with the Qubit RNA HS assay kit (Invitrogen, Carlsbad, CA, USA) and the Bioanalyzer RNA 6000 Nano kit (Agilent, Santa Clara, CA, USA). For qPCR analysis, RNA was primed with random primers and OligoDT and cDNA was prepared using the SuperScript III First-Strand Synthesis System (ThermoFisher Scientific). PCR was carried out on the LightCycler 480 (Roche, Basel, Switzerland) using the SYBR Green master mix (Roche). Gene expression was quantified relative to three housekeeping genes using the LightCycler 480 software (Roche) and the qBase+ software (Biogazelle, Ghent, Belgium).
Omni tissue homogeniser
Manufactured by Omni International
Sourced in United States
The Omni tissue homogeniser is a laboratory equipment designed to mechanically disrupt tissue samples for various analytical procedures. It utilizes a high-speed rotating blade to efficiently homogenise a wide range of biological materials, enabling the extraction and preparation of cellular components for further investigation.
Automatically generated - may contain errors
Lab products found in correlation
Superscript 3 first strand synthesis system, by Thermo Fisher Scientific (2 mentions)
Lightcycler 480, by Roche (2 mentions)
Nucleospin rna plus extraction kit, by Macherey-Nagel (1 mentions)
Bioanalyzer rna 6000 nano kit, by Agilent Technologies (1 mentions)
Qubit rna hs assay kit, by Thermo Fisher Scientific (1 mentions)
Qbase software, by CellCarta (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Sybr green master mix, by Roche (1 mentions)
Plastic hard tissue probes, by Omni International (1 mentions)
Magnetic luminex mouse premixed multi analyte kits, by R&D Systems (1 mentions)
Luminex 200 analyser, by DiaSorin (1 mentions)
2 protocols using omni tissue homogeniser
1
Quantitative Analysis of Bone Marrow Gene Expression
2
Cytokine Profiling of Murine Ear Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole ear pinnae were homogenised in 1.5 ml flat-bottom tubes containing 400 μl of 0.01% BSA/PBS with an OMNI Tissue Homogeniser with plastic hard-tissue probes (OMNI International, Kennesaw, GA, Georgia). The homogenate was then centrifuged at 10,000 rcf for 20 min at 4 °C and the supernatant was collected and stored at -80 °C. The levels of IFNγ, TNFα, IL-1β, IL-4, IL-6, IL-10, IL-33, CCL2, CCL3, CCL4, CCL5, CCL7, CCL20, CXCL1, CXCL2, CXCL5 and CXCL10 were measured using Magnetic Luminex Mouse Premixed Multi-Analyte kits (R&D Systems, Minneapolis, Minnesota) and a Luminex 200 analyser (Luminex Corporation, Austin, Texas).
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