Log In Sign up
The largest database of trusted experimental protocols

Stepone software

Manufactured by Bio-Rad
Visit Supplier
Sourced in United States

StepOne software is a real-time PCR data analysis software developed by Bio-Rad. It provides the core functionality to analyze and interpret data generated from real-time PCR experiments.

Automatically generated - may contain errors

Lab products found in correlation

Gdna eraser kit, by Takara Bio (1 mentions) Sybr green, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Rna lysis buffer, by Qiagen (1 mentions) Stepone real time pcr system, by Bio-Rad (1 mentions) Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Rnase free dnase, by Promega (1 mentions) Facsaria 2, by BD (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Sybr green master mix, by Bio-Rad (1 mentions)

Close spelling variants of this product

StepOne

2 protocols using stepone software

1

Retinal Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA from retinas and RGCs were extracted using Trizol reagent (Invitrogen, Carlsbad, CA, US), according to the manufacturer’s protocol. Approximately 40 ng total RNA was reverse transcribed using the PrimeScript RT Reagent Kit and genomic DNA (gDNA) Eraser Kit (RR047A; Takara, Dalian, Liaoning, China). The expression levels of lncRNAs, miRNAs, and mRNAs were detected using SYBR Green (#K0221; Thermo Scientific, Waltham, MA, US), and the ΔCt value was calculated and obtained using StepOne software (Bio-Rad, Hercules, CA, US). The sequences of lncRNA Mbd2-AL1 and miR-188-3p were retrieved from GenBank database (GenBank: 67895 and GenBank: 387183, respectively). Primer sequences were included in Table S3.
Ge Y., Zhang R., Feng Y, & Li H. (2020). Mbd2 Mediates Retinal Cell Apoptosis by Targeting the lncRNA Mbd2-AL1/miR-188-3p/Traf3 Axis in Ischemia/Reperfusion Injury. Molecular Therapy. Nucleic Acids, 19, 1250-1265.
+ Open protocol
+ Expand
2

RNA Isolation and qRT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Splenic derived LSK− cells and stromal cells sorted on an FACSAria II (Becton Dickinson) were subsequently resuspended in RNA lysis buffer (QIAGEN), and RNA was isolated using an RNeasy mini kit following the manufacturer’s protocol. The resulting RNA from each sample was treated with RNase-free DNase (Promega), followed by cDNA preparation using oligo-dT primers and SuperScript II Reverse Transcriptase (Life Technologies). For real-time qRT-PCR, cDNA from an equivalent number of cells was mixed with SYBR Green master mix (Bio-Rad) and appropriate primer sets; analysis was performed using a StepOne™ Real-Time PCR System and StepOne™ Software.
Ghosh D., Brown S.L, & Stumhofer J.S. (2017). IL-17 promotes differentiation of splenic LSK− lymphoid progenitors into B cells following Plasmodium yoelii infection. Journal of immunology (Baltimore, Md. : 1950), 199(5), 1783-1795.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!