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40um mesh filter

Manufactured by BD
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The 40μM mesh filter is a laboratory equipment component designed to filter particles or objects up to 40 micrometers in size. It serves as a separation tool for various applications that require the filtration of small-scale materials.

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Lab products found in correlation

Percoll gradient, by Merck Group (1 mentions) Dnase, by Merck Group (1 mentions) Collagenase, by Merck Group (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Verapamil, by Merck Group (1 mentions)

Close spelling variants of this product

Mesh filter (7 citations)

2 protocols using 40um mesh filter

1

Isolation of Lung Lymphocytes

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Lymphocytes were isolated from the lungs as previously described. Briefly, the mice were perfused with PBS, lungs excised, minced and incubated in 1mg/ml Collagenase and 1mg/ml DNase (both from Sigma-Aldrich) in 3ml of RPMI for 45 min at 37 degrees. Single cell suspensions were obtained by pushing the digested lung pieces through a 40uM mesh filter (BD biosciences). Lung lymphocytes were then purified by a 44/67% percoll gradient (Sigma-Aldrich and centrifugation at 2000rpm for 20 min at room temperature.
West E.E., Merle N.S., Kamiński M.M., Palacios G., Kumar D., Wang L., Bibby J.A., Overdahl K., Jarmusch A.K., Freeley S., Lee D.Y., Thompson J.W., Yu Z.X., Taylor N., Sitbon M., Green D.R., Bohrer A., Mayer-Barber K.D., Afzali B., Kazemian M., Scholl-Buergi S., Karall D., Huemer M, & Kemper C. (2023). Loss of CD4+ T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection. Immunity, 56(9), 2036-2053.e12.
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2

Hoechst 33342 Staining for Side Population

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The cells were labeled with Hoechst 33342 (Sigma, USA) at a concentration of 5 ug/ml for SMMC-7721, Huh7 and 8 ug/ml for MHCC-97H. The cells were incubated for 90 minutes at 37°C in the absence or presence of 60 ug/ml verapamil (Sigma, USA). After staining, the cells were suspended in cold DMEM that contained 2 ug/ml propidium iodide (PI, Sigma, USA), 2% FBS and 10 mM HEPES, then passed through a 40 um mesh filter (BD, USA) and maintained at 4°C until flow cytometry analysis.
Yang X., Wang J., Qu S., Zhang H., Ruan B., Gao Y., Ma B., Wang X., Wu N., Li X., Dou K, & Li H. (2015). MicroRNA-200a suppresses metastatic potential of side population cells in human hepatocellular carcinoma by decreasing ZEB2. Oncotarget, 6(10), 7918-7929.
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