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Apc conjugated anti mouse ly6g antibody

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The APC-conjugated anti-mouse Ly6G antibody is a laboratory reagent used to identify and quantify Ly6G-expressing cells, such as neutrophils, in mouse samples. The antibody is conjugated to the fluorescent dye allophycocyanin (APC), which allows for the detection and analysis of Ly6G-positive cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Cd11b apc cy7, by BD (6 mentions) Aria plus high speed sorter, by BD (2 mentions) Anti ly6g antibody 1a8, by BioXCell (1 mentions) Fluoview 1000 microscope, by Olympus (1 mentions)

Spelling variants of this product

Anti-Ly6G (64 citations) Ly6G-APC (23 citations) Anti-Ly6G antibody (8 citations) Anti-Ly6G-APC (7 citations) Anti-mouse Ly6G (7 citations) APC-conjugated anti-Ly6G (6 citations) APC-conjugated anti-mouse Ly6G (3 citations) Anti-mouse Ly6G-APC (2 citations) Mouse anti (2 citations)

6 protocols using apc conjugated anti mouse ly6g antibody

1

Isolation of Mouse Neutrophils

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Mouse neutrophils were isolated from bone marrow of tibias and femurs (18 ). Neutrophils were sorted on a BD-Aria-Plus high-speed sorter after incubation with APC-conjugated anti-mouse-Ly6G antibody (BD Biosciences) and APC-Cy7 CD11b (BD Biosciences) (purity >96% and >95% viability by Tryptan blue staining) (Supplementary Fig. 1).
Tohme S., Yazdani H.O., Al-Khafaji A.B., Chidi A.P., Loughran P., Mowen K., Wang Y., Simmons R.L., Huang H, & Tsung A. (2016). Neutrophil Extracellular Traps Promote the Development and Progression of Liver Metastases after Surgical Stress. Cancer research, 76(6), 1367-1380.
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2

Neutrophil Isolation and Depletion for I/R

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Mouse neutrophils were isolated from bone marrow of tibias and femurs as described previously (10 ). Neutrophils were sorted on a BD Aria Plus high-speed sorter after incubation with APC-conjugated anti-mouse Ly6G antibody and APC-Cy7 CD11b (BD Biosciences) (purity, >96%) (Supplementary Fig. 1). Neutrophil depletion was performed as described previously (14 ) with an intra-peritoneal injection of 500 μg anti-Ly6G antibody (1A8) (BioXCell) 24 and 2 hours before I/R. TLR9KO, TLR4 KO or WT freshly isolated neutrophils were injected into the spleens of WT mice just before I/R.
Huang H., Tohme S., Al-Khafaji A.B., Tai S., Loughran P., Chen L., Wang S., Kim J., Billiar T., Wang Y, & Tsung A. (2015). DAMPs-activated neutrophil extracellular trap exacerbates sterile inflammatory liver injury. Hepatology (Baltimore, Md.), 62(2), 600-614.
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3

Neutrophil Isolation from Murine Tissues

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Neutrophils were isolated from peripheral blood, spleen, bone marrow (BM) and mesenteric lymph nodes (mLN) of APC-WT and APC-KRASG12D mice. Neutrophils were sorted on a BD-Aria-Plus high-speed sorter after incubation with APC-conjugated anti-mouseLy6G antibody (BD Biosciences, San Jose, California, USA) and APC-Cy7 CD11b (BD Biosciences, San Jose, California, USA).
Shang A., Gu C., Zhou C., Yang Y., Chen C., Zeng B., Wu J., Lu W., Wang W., Sun Z, & Li D. (2020). Exosomal KRAS mutation promotes the formation of tumor-associated neutrophil extracellular traps and causes deterioration of colorectal cancer by inducing IL-8 expression. Cell Communication and Signaling : CCS, 18, 52.
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4

Neutrophil Extracellular Trap Formation

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Mouse neutrophils were isolated from the bone marrow of tibias and femurs from HMGB1 Flox and HMGB1 Pf4 mice. Neutrophils were sorted on a BD Aria Plus high-speed sorter after incubation with APC-conjugated anti-mouse Ly6G antibody and APC-Cy7 CD11b (BD Biosciences). Neutrophils were plated and allowed to adhere to coated plates for 1 h before stimulation with recombinant HMGB1 (1 μg/ml) or LPS (10 μg/ml). Neutrophils were then fixed in 2% paraformaldehyde and incubated with antibodies for CitH3 (1:50, Abcam, ab52013) and a DAPI nuclear stain. Confocal images were acquired using Olympus Fluoview 1000 microscope. NETs were identified by the presence of extracellular CitH3.
Dyer M.R., Chen Q., Haldeman S., Yazdani H., Hoffman R., Loughran P., Tsung A., Zuckerbraun B.S., Simmons R.L, & Neal M.D. (2018). Deep vein thrombosis in mice is regulated by platelet HMGB1 through release of neutrophil-extracellular traps and DNA. Scientific Reports, 8, 2068.
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5

Isolation and Purification of Mouse Neutrophils

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Mouse neutrophils were isolated from bone marrow extracted from the tibias and femurs. Neutrophils were sorted after incubation with allophycocyanin (APC) -conjugated anti-mouse-Ly6G antibody (BD Pharmingen™, Cat NO. 560599) and APC-Cy7 CD 11b (BD Pharmingen™, Cat NO. 557657).
Wang W.W., Wu L., Lu W., Chen W., Yan W., Qi C., Xuan S, & Shang A. (2021). Lipopolysaccharides increase the risk of colorectal cancer recurrence and metastasis due to the induction of neutrophil extracellular traps after curative resection. Journal of cancer research and clinical oncology, 147(9).
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6

Isolation and Purification of Mouse Neutrophils

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Mouse neutrophils were isolated from bone marrow extracted from the tibias and femurs. Neutrophils were sorted after incubation with allophycocyanin (APC) -conjugated anti-mouse-Ly6G antibody (BD Pharmingen™, Cat NO. 560599) and APC-Cy7 CD 11b (BD Pharmingen™, Cat NO. 557657).
Wang W., Wu L., Lu W., Chen W., Yan W., Qi C., Xuan S., & Shang A. (2021). Lipopolysaccharides Increase the Risk of Colorectal Cancer Recurrence and Metastasis Due to the Induction of Neutrophil Extracellular Traps After Curative Resection.
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