Sc 514

Manufactured by Enzo Life Sciences

SC-514 is a small molecule inhibitor that targets the IKK-β subunit of the IKK complex. It is commonly used in research applications to investigate the role of the NF-κB signaling pathway.

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Lab products found in correlation

Rapamycin, by Enzo Life Sciences (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Metformin, by Selleck Chemicals (1 mentions) Rad001, by Selleck Chemicals (1 mentions) Bml 257, by Enzo Life Sciences (1 mentions) Temozolomide, by Merck Group (1 mentions) Sc 514, by Abcam (1 mentions) Rad50, by Cell Signaling Technology (1 mentions) Pf184, by Bio-Techne (1 mentions) Ph2ax s139, by Cell Signaling Technology (1 mentions) Lomustine, by Enzo Life Sciences (1 mentions) Survivin, by Cell Signaling Technology (1 mentions) P atm s1981, by Cell Signaling Technology (1 mentions) Tpca 1, by Selleck Chemicals (1 mentions) Ikk 16, by Selleck Chemicals (1 mentions) Crystal violet, by Merck Group (1 mentions) 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Hydrogen peroxide solution, by Merck Group (1 mentions) N acetyl l cysteine, by Merck Group (1 mentions)

2 protocols using sc 514

Knockdown of CCKBR in A431 Cells

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The human epidermoid carcinoma A431 cell line, which overexpresses CCKBR, was generated and kindly provided by Dr. Luigi Aloj 23 (link). The A431 and A431/CCKBR cell lines were cultured in DMEM, whereas the rat pancreatic acinar AR42J cells (ECACC, UK) were grown in RPMI medium, supplemented with 10% FCS, 2 mM glutamine and antibiotics (0.1 mg/mL streptomycin, 100 IU penicillin) at 37 °C and 5% CO₂. For CCKBR-specific knock-down, duplex siRNAs against CCKBR or control duplex against luciferase (Microsynth) were used at a final concentration of 100 nM in Optimem (Gibco): CCKBRseq1 sense RNA 5′-UAUACGAGUAGUAGCACCAdTdT-3′, CCKBRseq2 sense RNA 5′-CCGCCAAAGGAUGGAGUACdTdT-3′ and control sense RNA 5′-CGUACGCGGAAUACUUCGAdTdT-3′. Cells at 60-80% confluence were transfected with siRNAs by using Lipofectamin 3000 according to the manufacturer's recommendations. RAD001 (Selleckchem), rapamycin (Enzo Life Sciences), BML-257(Enzo), SC-514 (Enzo) or metformin (Selleckchem) were diluted in DMSO or water, respectively.

Grzmil M., Qin Y., Schleuniger C., Frank S., Imobersteg S., Blanc A., Spillmann M., Berger P., Schibli R, & Behe M. (2020). Pharmacological inhibition of mTORC1 increases CCKBR-specific tumor uptake of radiolabeled minigastrin analogue [177Lu]Lu-PP-F11N. Theranostics, 10(24), 10861-10873.

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Comprehensive Protein Profiling for Cancer Research

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Antibodies against IKKβ, catalase, SOD1, p21, p27, p-Chk1(S345), Chk1, p-Chk2(T68), Chk2, p-H2AX(S139), H2AX, p-ATM(S1981), ATM, MGMT, PARP, Caspase-3, p-p53(S15), p53, survivin, XIAP, cIAP-1, cIAP-2, Mre11, Rad50, p95/NBS1, were purchased from Cell Signaling (Beverly, MA). Antibodies against Bak, Bcl-2, Bcl-xL, GAPDH, and actinin were purchased from Santa Cruz Biotechnology, Inc. Fotemustine and antibody against Flag (M2) was purchased from Sigma. 8-OHdG ELISA kit, mirin and SC-514 for in vivo experiment were purchased from Abcam. SC-514 for in vitro experiment, carmustine and lomustine were purchased from Enzo Life Sciences. PLX4032, BMS-345541, TPCA-1, LY-2409881, AZD3264, IKK-16 and PS-1145 were purchased form Selleckchem ML 120B dihydrochloride and PF184 were purchased from Tocris Bioscience. Crystal violet, propidium iodide (PI), N-acetyl-L-cysteine (NAC), temozolomide, 3-Amino-1,2,4-triazole (ATZ), hydrogen peroxide solution and 3-(4,5-Dimethyl-2-thiazolyl)−2,5-diphenyl-2H-tetrazolium bromide (MTT) were purchased from Sigma. 6-Carboxy-2′,7′-dichlorofluorescein diacetate (DCF) was purchased from Thermo Scientific.

Tse A.K., Chen Y.J., Fu X.Q., Su T., Li T., Guo H., Zhu P.L., Kwan H.Y., Cheng B.C., Cao H.H., Lee S.K., Fong W.F, & Yu Z.L. (2017). Sensitization of melanoma cells to alkylating agent-induced DNA damage and cell death via orchestrating oxidative stress and IKKβ inhibition. Redox Biology, 11, 562-576.

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