Mastercycle thermocycler

Manufactured by Eppendorf

Sourced in Germany

The Mastercycle thermocycler is a laboratory instrument designed for the amplification of DNA sequences through the Polymerase Chain Reaction (PCR) process. It precisely controls the temperature and timing of the thermal cycling steps required for DNA denaturation, primer annealing, and DNA extension.

Automatically generated - may contain errors

Lab products found in correlation

Typhoon fla 7000, by GE Healthcare (1 mentions)

Close spelling variants of this product

Mastercycle Gradient Thermocycler Mastercycle Thermocycler

2 protocols using mastercycle thermocycler

RNA Oligomer Assembly and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Equimolar amounts of RNA oligomers were mixed to a final concentration of 1 μM in TMS buffer (40 mM Tris-HCl, 10 mM MgCl₂, 100 mM NaCl). The mixture was heated to 80 °C for 5 min and then slowly cooled to 4 °C at a rate of 2 °C/min on an Eppendorf Mastercycle thermocycler. Confirmation of the assembly products were then run on native PAGE (described below).

Jasinski D.L., Khisamutdinov E.F., Lyubchenko Y.L, & Guo P. (2014). Physicochemically Tunable Polyfunctionalized RNA Square Architecture with Fluorogenic and Ribozymatic Properties. ACS Nano, 8(8), 7620-7629.

+ Open protocol

+ Expand

Synthesis of Aptamer-Functionalized RNA Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

The pRNA-3WJ nanoparticle harboring Sephadex aptamer and biotin (SEPapt/Bio-3WJ) was assembled by annealing three corresponding RNA strands at equal molar ratio in TMS buffer (40 mM Tris-HCl, 10 mM MgCl₂, 100 mM NaCl) by heating at 80°C for 5 min, followed by slowly cooling down to 4°C at a rate of 2°C/min on a Master cycle thermocycler (Eppendorf, Germany). The nanoparticle assembly was confirmed by 8% native polyacrylamide gel prepared in TBM buffer (89 mM Tris, 200 mM boric acid, 5 mM MgCl₂, pH 7.6). About 0.2 ug of RNA samples were loaded into each lane. After running at 80 V in a 4°C for 2h, the RNA nanoparticles were visualized by fluorescence signal or ethidium bromide staining, with images captured by Typhoon FLA 7000(GE Healthcare, UK).

Lee T., Yagati A.K., Pi F., Sharma A., Choi J.W, & Guo P. (2015). Construction of RNA-Quantum Dot Chimera for Nanoscale Resistive Biomemory Application. ACS nano, 9(7), 6675-6682.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!