Whole mount RNA in situ hybridisation (WISH) on zebrafish embryos was performed as previously described (Thisse et al., 1993 (link)). It is worth mentioning that treated and control embryos were hybridised together. The stat3 probe was obtained by PCR amplification from embryo cDNA using stat3_probe-fw (5′-TGCCACCAACATCCTAGTGT-3′) and stat3_probe-rv (5′-GCTTGTTTGCACTTTTGACTGA-3′) primers. The mt_nd2 probe was obtained by PCR amplification from embryo cDNA using mt_nd2-fw (5′-GCAGTAGAAGCCACCACAAA-3′) and mt_nd2-rv (5′-GGAATGCCGCGGATGTTATA-3′) primers. The pcna probe was obtained as described by Baumgart et al. (2014) (link). The sox9b probe was obtained as described by Chiang et al. (2001) (link). FISH was performed with FastBlue or TSA-amplification kit (Invitrogen) as described by Lauter et al. (2011) (link).
Tsa amplification kit
Manufactured by Thermo Fisher Scientific
The TSA-amplification kit is a laboratory equipment used for signal amplification in various molecular biology techniques. It enables the enhancement of target-specific signals without altering the target nucleic acid sequence. The kit provides the necessary reagents and protocols to perform this amplification process.
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2 protocols using tsa amplification kit
1
Whole Mount RNA In Situ Hybridization in Zebrafish
2
Whole Mount RNA In Situ Hybridization in Zebrafish
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Whole mount RNA in situ hybridization on zebrafish embryos was performed as previously described (Thisse et al., 1993) . It is worth mentioning that treated and control embryos were hybridized together. stat3 probe was obtained by PCR amplification from embryos cDNA using stat3_probe-fw (5'-TGCCACCAACATCCTAGTGT-3') and stat3_probe-rv (5'-GCTTGTTTGCACTTTTGACTGA-3') primers. mt_nd2 probe was obtained by PCR amplification from embryos cDNA using mt_nd2-fw (5'-GCAGTAGAAGCCACCACAAA-3') and mt_nd2-rv (5'-GGAATGCCGCGGATGTTATA-3') primers. pcna probe was obtained as described by Baumgart et al. (2014) . sox9b probe was obtained as described by Chiang et al. (2001) . her5 probe was obtained as described by Bally-Cuif, et al. (2000) .
her4 probe was obtained as described by Takke et al. (1999) . Fluorescence in situ hybridization was performed with FastBlue or TSA-amplification kit (Invitrogen) as described by Lauter et al. (2011) .
her4 probe was obtained as described by Takke et al. (1999) . Fluorescence in situ hybridization was performed with FastBlue or TSA-amplification kit (Invitrogen) as described by Lauter et al. (2011) .
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