Rabbit anti zeb1

Manufactured by Santa Cruz Biotechnology

Rabbit anti-Zeb1 is a primary antibody produced in rabbits that specifically binds to the Zeb1 protein. Zeb1 is a transcription factor involved in the regulation of gene expression. This antibody can be used in various biochemical and cell-based applications to detect and analyze Zeb1 in biological samples.

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Lab products found in correlation

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Spelling variants of this product

Anti-ZEB1 (25 citations) Rabbit anti-TM (2 citations)

2 protocols using rabbit anti zeb1

Immunofluorescence Analysis of Zeb1

Cited 1 time

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Tissues were fixed in Zn-formalin, paraffin embedded and stained as previously described15 (link). In brief, after sections were deparaffinized, rehydrated and subjected to antigen retrieval, sections were blocked in 5% donkey serum for 1 h at room temperature (RT), incubated with primary antibodies for 1 h at RT, washed, incubated with secondary antibodies for 1 h at RT, washed and mounted. Rabbit anti-Zeb1 (Santa Cruz Biotechnology, Santa Cruz, CA) required additional tyramide signalling amplification (PerkinElmer, Waltham, MA). See Supplementary Table 2 for a list of antibodies used. Slides were visualized using an Olympus IX71 inverted multicolour fluorescent microscope.

Aiello N.M., Bajor D.L., Norgard R.J., Sahmoud A., Bhagwat N., Pham M.N., Cornish T.C., Iacobuzio-Donahue C.A., Vonderheide R.H, & Stanger B.Z. (2016). Metastatic progression is associated with dynamic changes in the local microenvironment. Nature Communications, 7, 12819.

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Immunofluorescence staining of cells and tissues

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Tissues were fixed in Zn-formalin and embedded in paraffin prior to staining. Sections were deparaffinized, rehydrated and subjected to antigen retrieval. For staining cell lines, cells were fixed in 4% paraformaldehyde for 15 mins. For staining, sections and fixed cells were blocked in 5% donkey serum for 1 hour at room temperature (RT), incubated with primary antibodies for 1 hour at RT, washed, incubated with secondary antibodies for 1 hour at RT, washed and mounted. Primary antibodies used include goat anti-GFP (Abcam), rat anti-Ecadherin (Takara Bio), rabbit anti-Zeb1 (Santa Cruz), rabbit anti-Slug (gift of Dr. Joel Habener), rabbit anti-Vimentin (Cell Signaling Technologies), rabbit anti-Fsp1 (DAKO), rabbit anti-Rab5 (Cell Signaling Technologies), rabbit anti-Rab7 (Cell Signaling Technologies), rabbit anti-Rab11 (Cell Signaling Technologies), rabbit anti-EpCAM (Abcam), rabbit anti-Claudin-7 (Abcam), and rabbit anti-B-catenin (Cell Signaling Technologies). Zeb1 required additional tyramide signaling amplification (PerkinElmer). Slides were visualized using an Olympus IX71 inverted multicolor fluorescent microscope equipped with a DP71 camera. Select slides were also visualized using a Zeiss LSM 710 confocal microscope with Zen 2011 software.

Aiello N.M., Maddipati R., Norgard R.J., Balli D., Li J., Yuan S., Yamazoe T., Black T., Sahmoud A., Furth E.E., Bar-Sagi D, & Stanger B.Z. (2018). EMT subtype influences epithelial plasticity and mode of cell migration. Developmental cell, 45(6), 681-695.e4.

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