Cd150 clone mshad150

Manufactured by Thermo Fisher Scientific

Sourced in United States

The CD150 (Clone mShad150) is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is a cell surface receptor that functions as a co-stimulatory molecule. The core function of this product is to facilitate the study of cellular interactions and signaling pathways.

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Lab products found in correlation

Cd45 clone 104, by BioLegend (3 mentions) Cd48 clone hm48 1, by BioLegend (3 mentions) Ter119, by BioLegend (2 mentions) Sca 1, by BioLegend (2 mentions) Facsaria 3, by BD (2 mentions) Cd45.1 clone a20, by BD (2 mentions) Cd45.2 clone 104, by BioLegend (2 mentions) Cd11b, by BioLegend (1 mentions) Cd31 clone 390, by BD (1 mentions) Cytoflex flow cytometer, by Beckman Coulter (1 mentions)

3 protocols using cd150 clone mshad150

Comprehensive Hematopoietic Stem Cell Profiling

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All samples were analyzed by flow cytometry using a FACSAriaTM III or a LSR II (BD biosciences, San Jose, CA, USA). Lineage cocktail (include CD3, B220, Gr1, CD11b, Ter119; Biolegend), cKit (Clone 2B8, Biolegend), Sca1 (Clone D7, Biolegend), CD150 (Clone mShad150, eBioscience), CD48 (Clone HM48-1, Biolegend), CD45 (Clone 104) (Biolegend, San Diego, CA, USA), and CD31 (Clone 390, BD biosciences) antibodies were used.

Zhang H., Yeware A., Lee S, & Zhan H. (2021). A Murine Model With JAK2V617F Expression in Both Hematopoietic Cells and Vascular Endothelial Cells Recapitulates the Key Features of Human Myeloproliferative Neoplasm. Frontiers in Oncology, 11, 753465.

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Multiparametric flow cytometry for hematopoietic stem cells

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All samples were analyzed by flow cytometry using a FACSAriaTM III (BD biosciences, San Jose, CA, USA). CD45 (Clone 104) (Biolegend, San Diego, CA, USA), CD45.1 (Clone A20) (BD Biosciences), CD45.2 (Clone 104) (Biolegend), EPCR (CD201) (Clone eBio1560, eBioscience, San Diego, CA, USA), CD150 (Clone mShad150, eBioscience), and CD48 (Clone HM48–1, Biolegend) antibodies were used to enumerate CD45⁺EPCR(CD201)⁺CD48⁻CD150⁺ (E-SLAM) cells.[22 (link),23 (link)] MKs were sorted on the basis of CD41 expression (Clone MWReg30, Biolegend) and cell size.[15 (link)] CD45 (Clone 104) (Biolegend) and CD31 (Clone 390, BD biosciences) antibodies were used to isolate CD45⁻CD31⁺ marrow ECs.[24 (link)]

Zhang Y., Lin C.H., Kaushansky K, & Zhan H. (2018). JAK2V617F Megakaryocytes Promote Hematopoietic Stem/Progenitor Cell Expansion in Mice through Thrombopoietin/MPL signaling. Stem cells (Dayton, Ohio), 36(11), 1676-1684.

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Characterization of Hematopoietic Stem Cells

Cited 1 time

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All samples were analyzed by flow cytometry using a CytoFLEX flow cytometer (Beckman Coulter, Indianapolis, IN, USA). CD45 (Clone 104) (Biolegend, San Diego, CA, USA), CD45.1 (Clone A20) (BD Biosciences, San Jose, CA), CD45.2 (Clone 104) (Biolegend), Lineage (Lin) cocktail (include CD3, B220, Gr1, CD11b, Ter119; Biolegend), cKit (Clone 2B8, Biolegend), Sca1 (Clone D7, Biolegend), CD150 (Clone mShad150, eBioscience, San Diego, CA, USA), CD48 (Clone HM48-1, Biolegend), CD31 (Clone 390, BD Biosciences), and Ter119 (Clone Ter-119, Biolegend) antibodies were used. Intracellular CXCL12 expression and membranal SCF expression were analyzed using an anti-mouse CXCL12 antibody (Clone 79018, R&D Systems, Minneapolis, MN, USA) and an anti-mouse SCF antibody (Clone #40215, R&D Systems) as we previously described (Zhan et al., 2018 (link)).

Lee S, & Zhan H. (2024). Deciphering the differential impact of thrombopoietin/MPL signaling on hematopoietic stem/progenitor cell function in bone marrow and spleen. Stem Cell Reports, 19(2), 211-223.

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