Fasting blood samples of all participants were collected from the cubital vein between 7:00 a.m. and 9:00 a.m. Ethylenediaminetetraacetic acid plasma samples for the measurement of serum ACTH were stored at 2°C to 4°C before the test. Serum ACTH and DHEAS levels were measured by chemiluminescence on an Immulite 2000 analyzer (Siemens Healthcare Diagnostics Inc., Munich, Germany). The normal reference values of ACTH and DHEAS at 8:00 a.m. were <10.12 pmol/L and 350.0–4300.0 µg/L, respectively. Serum cortisol, T, LH, and FSH levels were measured by chemiluminescence on an ADVIA Centaur XP Analyzer (Siemens Healthcare Diagnostics, Inc., Co Dublin, Ireland); and normal reference values were as follows: cortisol 198.7–797.5 nmol/L, T 8.4–28.7 nmol/L, LH 1.5–9.3 mU/ml, and FSH 1.4–18.1 U/L. Other laboratory examinations included the tests for alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), fasting blood glucose (FBG), total cholesterol (TC), total triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and creatinine (Cr) levels. All the processes of drawing blood were successful without any stress. All assays were performed according to the manufacturers' recommendations. All biochemical determinations were conducted in the same laboratory with standard methods.
Advia centaur xp analyzer
Manufactured by Siemens
Sourced in Germany, United States
The ADVIA Centaur XP analyzer is a high-throughput, automated immunoassay system designed for clinical laboratories. It is capable of performing a wide range of diagnostic tests, including those for infectious diseases, hormones, and cardiac markers. The analyzer utilizes chemiluminescent technology to provide accurate and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Advia 2400 chemistry system, by Siemens (3 mentions)
Immulite 2000 analyzer, by Siemens (2 mentions)
Advia centaur insulin assay, by Siemens (2 mentions)
Discovery mr750, by GE Healthcare (1 mentions)
Cobas e601, by Roche (1 mentions)
Vacutainer sst 2 advance, by BD (1 mentions)
Ghrt 89hk, by Merck Group (1 mentions)
3 isobutyl 1 methylxanthin ibmx, by Merck Group (1 mentions)
Architect i2000sr analyzer, by Abbott (1 mentions)
Advia 1800 analyzer, by Siemens (1 mentions)
Architect galectin 3, by Abbott (1 mentions)
Architect bnp, by Abbott (1 mentions)
Liaison autoimmunoanalyzer, by DiaSorin (1 mentions)
Celltiter glo luminescent cell viability assay, by Promega (1 mentions)
Advia centaur xp, by Siemens (1 mentions)
Elf test, by Siemens (1 mentions)
Elf test kits, by Siemens (1 mentions)
18 protocols using advia centaur xp analyzer
1
Comprehensive Hormonal and Metabolic Profiling
2
Analyzing Blood Parameters and Tumor Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole blood samples were collected in ethylenediaminetetraacetic acid vials and hemoglobin, white blood cell count and platelet indices were analyzed with Beckman coulter LH-750/Sysmax XN-1000 coulter. In Sysmax XN-1000 five-part differential cell counters, the impedance principle is used for platelet count. Impedance counting discriminates particles based on their size. As platelets pass singly through an aperture between positive and negative electrodes, passing of the cell reverses the resistance and a pulse is generated which is recorded. Each pulse size is proportional to the size of platelets. Increase in PDW is an indication of platelet anisocytosis. All samples with abnormal values were also cross-checked by peripheral blood smear examination for which Leishman stain was used. Serum tumor markers- CA19-9, CEA and CA 125 were analyzed by chemiluminescence assay on Siemens Advia Centaur XP analyzer.
3
Pituitary Adenoma Evaluation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sellar MRI studies were acquired on a 3.0-T scanner (Discovery MR750, GE Healthcare). Maximum tumor diameter was measured, and tumors were classified as either microadenomas (< 10 mm) or macroadenomas (≥ 10 mm). Knosp grades 22 were used to define cavernous sinus invasion (Knosp grade > 2) based on the coronal T1-weighted contrast-enhanced images.
GH and IGF-1 concentrations were measured with chemiluminescence assays (L2KGRH2 [GH] and L2KGFZ [IGF-1], Siemens Healthcare Diagnostics Products Ltd.) and the Immulite 2000 analyzer. IGF-1 concentration in this study was expressed as the ratio of the IGF-1 level to the age-and sex-matched ULN (IGF-1/ULN) instead of the absolute IGF-1 level (ng/ml) for uniform reporting.
Other pituitary hormones were assessed using electrochemiluminescence assays (Roche Diagnostics GmbH) with the ADVIA Centaur XP analyzer (Siemens Healthineers). Prolactin (PRL) above the reference range confirmed the diagnosis of hyperprolactinemia. 23 Central hypogonadism, hypothyroidism, and hypoadrenalism were diagnosed on the basis of clinical signs and symptoms, low levels of end-organ hormones, and low/normal levels of pituitary hormones. [24] [25] [26]
GH and IGF-1 concentrations were measured with chemiluminescence assays (L2KGRH2 [GH] and L2KGFZ [IGF-1], Siemens Healthcare Diagnostics Products Ltd.) and the Immulite 2000 analyzer. IGF-1 concentration in this study was expressed as the ratio of the IGF-1 level to the age-and sex-matched ULN (IGF-1/ULN) instead of the absolute IGF-1 level (ng/ml) for uniform reporting.
Other pituitary hormones were assessed using electrochemiluminescence assays (Roche Diagnostics GmbH) with the ADVIA Centaur XP analyzer (Siemens Healthineers). Prolactin (PRL) above the reference range confirmed the diagnosis of hyperprolactinemia. 23 Central hypogonadism, hypothyroidism, and hypoadrenalism were diagnosed on the basis of clinical signs and symptoms, low levels of end-organ hormones, and low/normal levels of pituitary hormones. [24] [25] [26]
4
Fetal Growth Monitoring with Biochemical Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Weight and length z-scores and percentiles were calculated using the Fenton growth reference [35 (link)]. Patients were weighed before their first feed in the morning. Concentrations of P, Ca, Mg, and ALP were determined using standard procedures with the Advia 2400 Analyzer (Siemens Diagnostic Systems, Erlangen, Germany), and 25-OHD and PTH with the Advia Centaur XP Analyzer (Siemens Healthcare Diagnostics, Erlangen, Germany).
5
Bone Biomarker Measurements
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Concentrations of Ca and P were determined by standard procedures with the Advia 2400 Analyzer (Siemens Healthcare Diagnostic Systems, Erlangen, Germany); 25-OHD with the Advia Centaur XP Analyzer (Siemens Healthcare Diagnostics, Erlangen, Germany); PTH with Roche Cobas E 601 (Roche Diagnostic, Indianapolis, IN, USA); calcitonin with INMULITE 2000 (Siemens Healthcare Diagnostics, Erlangen, Germany); and calcitriol by radioimmunoassay in Reference Laboratory S.A. (Hospitalet de Llobregat, Spain).
6
Vitamin D Measurement in Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were placed in gel-containing tubes with a clot activator (BD Vacutainer SST II Advance, USA) and centrifuged at 1500 g for 10 minutes to separate serum from clot. Serum 25 (OH) vitamin D was measured by chemiluminescence immunoassay on an Advia Centaur XP analyzer (Siemens Healthineers, Erlangen, Germany). The intra-assay and inter-assay coefficients of variation for the 25 (OH) vitamin D assay were less than 8% and 12%, respectively.
7
Glucose, Insulin, and Gut Hormone Measurements
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PG was determined every 5 min during the clamp period and more frequently during MSF, by the glucose oxidase method using a glucose analyzer (Yellow Springs Instruments model 2300 STAT Plus Analyzer; YSI, Yellow Springs, OH). Serum insulin and C-peptide concentrations were quantified by routine immunoassays (Siemens Healthcare Diagnostics, Ballerup, Denmark) using the ADVIA Centaur XP analyzer at the Department of Clinical Biochemistry, Gentofte Hospital, Denmark. The intraserial coefficients of variation were 3% for insulin and between 2.5 and 5% for C-peptide. Plasma for analysis of PP, glucagon, GIP, and GLP-1 was extracted with ethanol (70% final concentration) before analysis. PP was assayed by using a midregion-specific antibody code no. HYB 347-07 (Statens Serum Institut, Copenhagen, Denmark), with human PP standards and 125 Ilabeled human PP (Perkin Elmer, Boston, MA) (19) . Total GLP-1 levels were assayed by using antiserum code no. 89390, which requires the intact amidated COOH-terminus of the molecule (47) . Glucagon was measured by using the COOH-terminally directed antiserum, code no. 4305, which detects glucagon of pancreatic origin (34) . Total GIP was measured by using a COOH-terminally directed antiserum code no. 80 867 (41) . Total ghrelin was measured with a RIA kit from Millipore (GHRT-89HK). Quality controls included in each assay fell within acceptable limits.
8
Cardiac Troponin I and Mortality
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All tests were performed using Siemens ADVIA Centaur XP analyzer (Munich, Germany), which has the lowest cTnI analytical sensitivity of 0.006 ng/ml, upper limit normal (ULN) with 99th percentile level of 0.040 ng/ml, and coefficient of variation < 10% at 0.030 ng/ml7 (link). The maximal cTnI value within 24 h of the emergency department visit was used for analysis.
The study index day was the day of the patient’s visit to the emergency department. Life status and cause of death were validated by the National Statistics of Korea. No patient was lost to follow-up with respect to death. The main cause of death was determined by the death codes in the 10th revision of the International Statistical Classification of Diseases and Related Health Problems (ICD-10). Primary outcome of interest was 360-day cardiac or non-cardiac deaths.
The study index day was the day of the patient’s visit to the emergency department. Life status and cause of death were validated by the National Statistics of Korea. No patient was lost to follow-up with respect to death. The main cause of death was determined by the death codes in the 10th revision of the International Statistical Classification of Diseases and Related Health Problems (ICD-10). Primary outcome of interest was 360-day cardiac or non-cardiac deaths.
9
Insulin Secretion Measurement in NT-3 and NT-18 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The measurement of secreted insulin was performed as previously described. Briefly, ADVIA Centaur Insulin Assay (REF 02230141) and ADVIA Centaur XP analyzer (Siemens Healthcare, Erlangen, Germany) were used. In vitro samples were obtained from supernatants of NT-3 and NT-18 LM cell cultures treated with panobinostat for 24 h and 48 h. Afterwards, stimulation with 500 μmol/L 3-Isobutyl-1-methylxanthin (IBMX, Sigma) for 60 min was initiated. No additional growth factors were added and untreated cells were used as control.
10
Biomarker Measurement in Cardiovascular Disease
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the time of admission, blood samples were collected into tubes containing ethylenediaminetetraacetic acid and then transported to the Biochemistry Department of Cho Ray Hospital to be processed and frozen at -80ºC for eventual measurements of galectin-3, BNP, troponin I, and albumin. Galectin-3 was analyzed using ARCHITECT galectin-3 assay, which is a two-step immunoassay procedure (ARCHITECT galectin-3, Abbott, Park, IL, USA) on the Architect i2000SR analyzer (Abbott Laboratories, Park, IL, USA), according to the manufacturer's instructions 22 (link) . BNP was analyzed using AR-CHITECT BNP (ARCHITECT BNP, Abbott) assay on the Architect i2000SR analyzer (Abbott Laboratories). Troponin I was analyzed using chemiluminescent microparticle immunoassay on the ADVIA Centaur XP analyzer (Siemens Diagnostics, Tarrytown, NY, USA). Albumin was analyzed using the colorimetric method on the ADVIA 1800 analyzer (Siemens Diagnostics, Tarrytown, NY, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!