β nicotinamide adenine dinucleotide phosphate hydrate nadp

Adenosine 5′-triphosphate disodium salt hydrate (≥99%), chlorophenol red-β-d-galactopyranoside (CPRG), β-nicotinamide adenine dinucleotide phosphate hydrate (NADP⁺), magnesium chloride hexahydrate (≥99%), d-(+)-glucose (≥99%), dimethyl sulfoxide (ACS reagent (≥99.9%)), d-glucose-6-phosphate sodium salt, N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES, ≥99.5%), imidazole (96.0%), Terrific broth modified, and triethanolamine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from CellGro Technologies, LLC (Lincoln, NE, USA). Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase (LmG6PDH) was purchased from Worthington Biochemical Corporation (Lakewood, NJ, USA). NADPH tetrasodium salt (≥95%) was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). For the 3-nitro-2-phenyl-2H-chromene analogues, compounds 1–7 were purchased from TimTec, LLC (Tampa, FL, USA), while compounds 8–13 were purchased from ChemDiv, Inc. (San Diego, CA, USA). Ethylenediaminetetraacetic acid (EDTA) tetrasodium salt hydrate (98%), Luria-Bertani (LB) broth, lysozyme (type VI), protease inhibitor tablets (EDTA-free), glycerol, and all other chemicals were purchased from Fisher Scientific (Hampton, NH, USA).

(±)-Doxazosin mesylate [(±)-DOX], (+)-doxazosin mesylate [(+)-DOX], and (−)-doxazosin mesylate [(−)-DOX] standards (>99.9% purity) were provided by the New Drug Research and Development Center of the North China Pharmaceutical Group Corporation (Shijiazhuang, China). β-Nicotinamide adenine dinucleotide phosphate hydrate (NADP), glucose-6-phosphate dehydrogenase from Baker’s yeast (S. cerevisiae), glucose-6-phosphate, prazosin (internal standard, IS), phenacetin, tolbutamide, and human CYP enzymes (CYP3A4, CYP2D6, CYP2C19, CYP2C8, CYP1A2, CYP2E1, and CYP2C9) were purchased from Sigma-Aldrich (St Louis, USA). Dextromethorphan hydrobromide, paclitaxel, and testosterone were provided by TCI Shanghai (Shanghai, China). Chlorzoxazone and omeprazole were purchased from J&K Chemical (Beijing, China). Heparin sodium injection was provided by the Jiangsu Wanbang Biochemical Pharmaceutical Group Co., Ltd. (Xuzhou, China). Human and rat liver microsomes were purchased from BD Gentest (Franklin, USA). Tris base and MgCl₂ were purchased from Tianjin Yongda Chemical Reagent Co., Ltd. (Tianjin, China). Ultrapure water was prepared by Thermo Scientific Nanopure Water Purifier (Waltham, USA). HPLC-grade methanol and acetonitrile were purchased from Thermo Fisher Scientific (Ottawa, ON, Canada). All the other chemicals were of analytical grade.