Liver tissues in each group were fixed in formaldehyde over 48 h, then embedded in paraffin and forward sliced into 5-μm-thick sections. According to the standard procedures, liver tissues slices were subjected to H&E staining and Sirius Red staining.
For IHC staining of α-SMA and Collagen I, mice liver tissues slice staining was performed as standard protocol described. During the assay, anti-α-SMA (Abcam, USA) and anti-Collagen I (Absin, China) were used as primary antibody and HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody and 3,3′-Diaminobenzidine (DAB) used as the chromogen (ZSGO-BIO, China).
For immunofluorescence staining, paraffin-embedded liver tissues slice was proceeded with standard protocols and then co-stained with primary antibodies anti-mouse ALB antibody (1:500, NB600-41,532, Novus Biologicals, USA) and anti-Glul antibody (1:500, ABclonal, China) and incubated with fluorescence secondary antibody, 4′,6-diamidino-2-phenylindole (DAPI) for nucleus staining.
All images were captured by upright fluorescence microscope OLYMPUS BX53.
For IHC staining of α-SMA and Collagen I, mice liver tissues slice staining was performed as standard protocol described. During the assay, anti-α-SMA (Abcam, USA) and anti-Collagen I (Absin, China) were used as primary antibody and HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody and 3,3′-Diaminobenzidine (DAB) used as the chromogen (ZSGO-BIO, China).
For immunofluorescence staining, paraffin-embedded liver tissues slice was proceeded with standard protocols and then co-stained with primary antibodies anti-mouse ALB antibody (1:500, NB600-41,532, Novus Biologicals, USA) and anti-Glul antibody (1:500, ABclonal, China) and incubated with fluorescence secondary antibody, 4′,6-diamidino-2-phenylindole (DAPI) for nucleus staining.
All images were captured by upright fluorescence microscope OLYMPUS BX53.