Log In Sign up
The largest database of trusted experimental protocols

Er 6f11

Manufactured by Leica
Visit Supplier
Sourced in United Kingdom

The ER (6F11) is a Leica lab equipment product. It is designed to perform specific laboratory functions. Further details about its core function or intended use are not available.

Automatically generated - may contain errors

Lab products found in correlation

Ck5 xm26, by Leica (2 mentions) Envision polymer hrp anti mouse 3 3 diaminobenzidine dab, by Agilent Technologies (2 mentions) Toto 3, by Thermo Fisher Scientific (1 mentions) Aldh1 44, by BD (1 mentions) Vimentin r28, by Cell Signaling Technology (1 mentions) Mib 1, by Agilent Technologies (1 mentions) P53 do7, by Leica (1 mentions) Bond 3 system, by Leica (1 mentions) Streptavidin peroxidase, by Agilent Technologies (1 mentions) Ki 67 mib 1, by Agilent Technologies (1 mentions) Hybond pvdf membrane, by GE Healthcare (1 mentions) Anti β actin ac 15, by Merck Group (1 mentions) Ecl plus western blotting detection reagent, by GE Healthcare (1 mentions) Chemidoc xrs system, by Bio-Rad (1 mentions) M per mammalian protein extraction reagent, by Thermo Fisher Scientific (1 mentions) Rm 9106, by Thermo Fisher Scientific (1 mentions) Ncl l er 6f11, by Leica (1 mentions) Pt link, by Agilent Technologies (1 mentions) Ndp view software, by Hamamatsu Photonics (1 mentions) Nanozoomer digital pathology scanner, by Hamamatsu Photonics (1 mentions)

7 protocols using er 6f11

1

Characterization of Breast Cancer Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
BrCSCs were fixed with 2% paraformaldehyde for 30 minutes at 37°C and incubated O.N. at 4°C with the following primary antibodies: ALDH1 (44, BD), CK5 (XM26, Novocastra), CK14 (LL002, Novocastra), CK8-18 (CD10, Novocastra), MUC1 (BD Pharmigen), VIMENTIN (R28, Cell Signaling), ER (6F11, Novocastra). Thereafter, cells were labeled with secondary antibodies (Invitrogen). Nuclei were counterstained with Toto-3 (Invitrogen).
Petrelli A., Carollo R., Cargnelutti M., Iovino F., Callari M., Cimino D., Todaro M., Mangiapane L.R., Giammona A., Cordova A., Montemurro F., Taverna D., Daidone M.G., Stassi G, & Giordano S. (2014). By promoting cell differentiation, miR-100 sensitizes basal-like breast cancer stem cells to hormonal therapy. Oncotarget, 6(4), 2315-2330.
+ Open protocol
+ Expand
2

Histopathological and Immunohistochemical Analysis of FFPE Breast Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used formalin-fixed, paraffin-embedded (FFPE) tissue for histopathologic analysis and immunohistochemical (IHC) staining. All of the FFPE tissues were stained with hematoxylin and eosin. The detailed histopathologic features were reviewed by three breast pathologists (H.L., Y.A.C., and E.Y.C.). IHC staining was performed on 4-μm sections of FFPE tissue using a Leica BOND-III system (Leica Biosystems, Wetzlar, Germany) to stain for estrogen receptor (ER; 6F11, 1:300, Novocastra, Newcastle upon Tyne, UK), progesterone receptor (clone 16, 1:1,200, Novocastra), p53 (DO7, 1:1,200, Leica Biosystems), Ki-67, (MIB1, 1:200, Dako, Carpinteria, CA), and cytokeratin 5/6 (D5/16B4, 1:100, Dako) and the Ventana BanchMark XT platform (Ventana Medical Systems Inc., Tucson, AZ) to stain for HER2 (4B5, Ventana Medical Systems Inc.) according to the manufacturers’ protocols.
Lee H., Cho Y.A., Kim D.G, & Cho E.Y. (2023). Next-Generation Sequencing in Breast Cancer Patients: Real-World Data for Precision Medicine. Cancer Research and Treatment : Official Journal of Korean Cancer Association, 56(1), 149-161.
+ Open protocol
+ Expand
3

Immunohistochemical Profiling of Breast Cancer Xenografts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical analyses were carried out on 5-µm-thick paraffin-embedded sections of breast cancer xenografts. Tissues underwent antigen retrieval, where required permeabilized with cold 0.1% Triton X 100, and stained overnight at 4 °C with antibodies against Ki67 (MIB 1, Dako), p63 (4A4, Santa Cruz), CK5 (XM26, Novocastra), CK 5/6 (D5/16B4, Dako), CK8/18 (5D3, Novocastra), Vimentin (R28), PR (16, Biocare Medical), Her2 (D8F12, CST), and ER (6F11, Novocastra). Successively, sections were incubated with biotinylated secondary antibodies and exposed to streptavidin-peroxidase (Dako). Stainings were revealed with 3-amino-9-ethylcarbazole substrate (AEC, Dako) and nuclei counterstained with aqueous hematoxylin. For H/E, tissues were stained with hematoxylin for 2 min and subsequently with eosin for 1 min.
Poli V., Fagnocchi L., Fasciani A., Cherubini A., Mazzoleni S., Ferrillo S., Miluzio A., Gaudioso G., Vaira V., Turdo A., Gaggianesi M., Chinnici A., Lipari E., Bicciato S., Bosari S., Todaro M, & Zippo A. (2018). MYC-driven epigenetic reprogramming favors the onset of tumorigenesis by inducing a stem cell-like state. Nature Communications, 9, 1024.
+ Open protocol
+ Expand
4

Protein Expression Analysis of MCF-7 and MDA-MB-453 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The whole‐cell proteins from MCF‐7 and MDA‐MB‐453 cells were extracted using M‐PER mammalian protein extraction reagent (Thermo Fisher Scientific Pierce Biotechnology, Rockford, IL). The lysate proteins (10  μg) were subjected to SDS–PAGE (10% acrylamide gel). Following SDS–PAGE, the proteins were transferred onto Hybond PVDF membranes (GE Healthcare, Buckinghamshire, UK). Primary antibody used was anti‐TACC2 antibody (Gene Tex), ER (6F11, Novocastra, Newcastle upon Tyne, UK) and AR (AR441, DAKO). In addition, anti‐β‐actin (AC‐15, Sigma‐Aldrich) antibody was used as an internal control. Antibody‐protein complexes on the blots were detected using ECL‐Plus Western Blotting Detection Reagents (GE Healthcare), and the protein bands were visualized using ChemiDoc™ XRS+ system (Bio‐Rad Laboratories, Inc., Hercules, CA).
Onodera Y., Takagi K., Miki Y., Takayama K., Shibahara Y., Watanabe M., Ishida T., Inoue S., Sasano H, & Suzuki T. (2016). TACC2 (transforming acidic coiled‐coil protein 2) in breast carcinoma as a potent prognostic predictor associated with cell proliferation. Cancer Medicine, 5(8), 1973-1982.
+ Open protocol
+ Expand
5

Immunohistochemistry Protocol for Tissue Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunohistochemistry (IHC) cut tissue sections (5μm) on charged glass slides were baked for 10–12 hours at 58°C in a dry slide incubator, deparaffinized in xylene and rehydrated via an ethanol step gradient. Heat-induced antigen retrieval steps were performed at pH 9.0 for all targets. All primary antibodies were incubated at room temperature for 1 hour [clone, manufacturer, dilution: Her2 (SP3, Neomarkers, 1:100); ER (6F11, Leica, 1:200); CD3 (polyclonal, Dako, 1:100)] followed by a standard chromogenic staining protocol with the Envision Polymer-HRP anti-mouse/3,3′diaminobenzidine (DAB, Dako) process. Slides were counterstained in Harris hematoxylin. Immunohistochemistry scoring was performed using established guidelines, when appropriate. All IHC results were evaluated against positive and negative tissue controls.
Krug K., Jaehnig E.J., Satpathy S., Blumenberg L., Karpova A., Anurag M., Miles G., Mertins P., Geffen Y., Tang L.C., Heiman D.I., Cao S., Maruvka Y.E., Lei J.T., Huang C., Kothadia R.B., Colaprico A., Birger C., Wang J., Dou Y., Wen B., Shi Z., Liao Y., Wiznerowicz M., Wyczalkowski M.A., Chen X.S., Kennedy J.J., Paulovich A.G., Thiagarajan M., Kinsinger C.R., Hiltke T., Boja E.S., Mesri M., Robles A.I., Rodriguez H., Westbrook T.F., Ding L., Getz G., Clauser K.R., Fenyö D., Ruggles K.V., Zhang B., Mani D.R., Carr S.A., Ellis M.J, & Gillette M.A. (2020). Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy. Cell, 183(5), 1436-1456.e31.
+ Open protocol
+ Expand
6

Immunohistochemical Analysis of Mammary Gland

Check if the same lab product or an alternative is used in the 5 most similar protocols
Paraffin wax-embedded transverse sections (5 μm) from formalin-fixed mammary gland specimens were stained using anti-Ki-67 (RM-9106, 1/100; Thermo Fisher Scientific), anti-PR (sc-7208, 1/50; Santa Cruz Biotechnology) antibodies or anti active caspase-3 (AF835, 1/800; R&D Systems) as previously described (Abot et al., 2013 (link)). For ERα detection (ER-6F11, NCL-L-ER-6F11, Leica), immunohistochemistry was performed with a Dako Autostainer Link 48 on 3 μm sections. Antigen retrieval was performed using a Dako PT Link pressure cooker in pH 6.0 citrate buffer and an EnVision system for antibody detection. Images were acquired using a NanoZoomer Digital Pathology Scanner and NDPView software (Hamamatsu Photonics) for quantification.
Gagniac L., Rusidzé M., Boudou F., Cagnet S., Adlanmerini M., Jeannot P., Gaide N., Giton F., Besson A., Weyl A., Gourdy P., Raymond-Letron I., Arnal J.F., Brisken C, & Lenfant F. (2020). Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium. Development (Cambridge, England), 147(5), dev182303.
+ Open protocol
+ Expand
7

Immunohistochemistry Protocol for Tissue Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunohistochemistry (IHC) cut tissue sections (5μm) on charged glass slides were baked for 10–12 hours at 58°C in a dry slide incubator, deparaffinized in xylene and rehydrated via an ethanol step gradient. Heat-induced antigen retrieval steps were performed at pH 9.0 for all targets. All primary antibodies were incubated at room temperature for 1 hour [clone, manufacturer, dilution: Her2 (SP3, Neomarkers, 1:100); ER (6F11, Leica, 1:200); CD3 (polyclonal, Dako, 1:100)] followed by a standard chromogenic staining protocol with the Envision Polymer-HRP anti-mouse/3,3′diaminobenzidine (DAB, Dako) process. Slides were counterstained in Harris hematoxylin. Immunohistochemistry scoring was performed using established guidelines, when appropriate. All IHC results were evaluated against positive and negative tissue controls.
Krug K., Jaehnig E.J., Satpathy S., Blumenberg L., Karpova A., Anurag M., Miles G., Mertins P., Geffen Y., Tang L.C., Heiman D.I., Cao S., Maruvka Y.E., Lei J.T., Huang C., Kothadia R.B., Colaprico A., Birger C., Wang J., Dou Y., Wen B., Shi Z., Liao Y., Wiznerowicz M., Wyczalkowski M.A., Chen X.S., Kennedy J.J., Paulovich A.G., Thiagarajan M., Kinsinger C.R., Hiltke T., Boja E.S., Mesri M., Robles A.I., Rodriguez H., Westbrook T.F., Ding L., Getz G., Clauser K.R., Fenyö D., Ruggles K.V., Zhang B., Mani D.R., Carr S.A., Ellis M.J, & Gillette M.A. (2020). Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy. Cell, 183(5), 1436-1456.e31.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!