Hplc ms grade

All solvents used for sample preparation were HPLC‐MS grade (Fisher Scientific, Fair Lawn, NJ). Nonporous graphitized carbon solid‐phase extraction (GCC‐SPE) (2000 μg binding capacity) was purchased from Glygen Corp. (Columbia, MD, USA). Oligosaccharide standards with a minimum purity of 95% (lacto‐N‐tetraose, LNT; lacto‐N‐neotetraose, LNnT; lacto‐N‐hexaose, LNH; lacto‐N‐neohexaose, LNnH; acetylgalactosaminyl‐α1,3‐galactose‐β‐1,4‐glucose, 2 Hex – 1 HexNAc; galactose‐α ‐1,3‐galactose‐β‐1,4‐glucose, 3 Hex; 6′‐sialyllactosamine, 6′‐SLN; 3′‐sialyllactosamine, 3′‐SLN; 6′‐sialyllactose, 6′‐SL; 3′‐sialyllactose, 3′‐SL) were purchased from V‐Labs (Covington, LA, USA). Nanopure water (18.2MΩ.cm, 25 °C) was used for the analytical work.

All solvents used throughout were HPLC-MS grade from Fisher Scientific (Hampton, NH). Butylhydroxytoluene (BHT) used during extraction and HPLC-grade sodium acetate used during HPLC-MS/MS analysis were obtained from Sigma Aldrich (Darmstadt, Germany). Standards for linoleic acid (LA); arachidonic acid (AA); heptadecanoic acid (C17:0); ±13-hydroperoxy-9Z,11E-octadecadienoic acid (HPODE); ±13-hydroxy-9Z,11E-octadecadienoic acid (HODE); 9-oxo-10E,12Z-octadecadienoic acid (KODE); ±12-hydroperoxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (HPETE); ±5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (HETE); and 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (KETE) were purchased from Cayman Chemical (Ann Arbor, MI). All FA standards were used without further purification.
3T3-L1 preadipocytes were acquired from Zen-Bio (Research Triangle Park, NC). High glucose DMEM (with and without phenol red), 100x antibiotic-antimycotic, and newborn calf serum were obtained from Gibco (Hampton, NH). Fetal bovine serum was purchased from Gemini Bio-Products (West Sacramento, CA). The isobutylmethylxanthine and dexamethasone used for differentiation were from Sigma Aldrich (Darmstadt, Germany). Humulin R regular recombinant human insulin was procured from Eli Lilly (Indianapolis, IN).