Paraformaldehyde was obtained from Affymetrix (Santa Clara, CA). Anti-GITR, anti-GITRL antibodies and the respective isotype control were from R&D Systems (Minneapolis, MN). CD19-FITC, CD41a-PECy5, CD41a-PE, PAC-1-FITC, CD61-FITC and CD62P-FITC were from BD Pharmingen (San Diego, CA), CD3-APC/Fire and CD56-PeCy7 were from Biolegend (San Diego, CA). The goat anti-mouse PE conjugate was from Dako (Glostrup, Denmark). Biocoll Separating Solution was purchased from Biochrom AG (Berlin, Germany). VPA was from Sigma-Aldrich (St. Louis, MO). Thrombin Receptor Activator Peptide 6 (TRAP-6), collagen and ADP were purchased from Sigma-Aldrich (St. Louis, MO).
Cd3 apc fire
Manufactured by BioLegend
The CD3-APC/Fire is a fluorochrome-conjugated antibody that binds to the CD3 antigen, which is expressed on the surface of T cells. This product can be used in flow cytometry applications to detect and analyze T cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Cd56 pe cy7, by BioLegend (2 mentions)
Cd62p fitc, by BD (2 mentions)
Cd41a pecy5, by BD (2 mentions)
Paraformaldehyde (pfa), by Thermo Fisher Scientific (2 mentions)
Goat anti mouse pe conjugate, by Agilent Technologies (2 mentions)
Cd41a pe, by BD (1 mentions)
Biocoll separating solution, by Harvard Bioscience (1 mentions)
Cd61 fitc, by BD (1 mentions)
Adenosine diphosphate (adp), by Merck Group (1 mentions)
Cd19 fitc, by BD (1 mentions)
Pac 1 fitc, by BD (1 mentions)
Cd69 pe, by BioLegend (1 mentions)
Anti human gitrl antibody, by R&D Systems (1 mentions)
Bicoll separating solution, by Harvard Bioscience (1 mentions)
Cd25 fitc, by BioLegend (1 mentions)
2 protocols using cd3 apc fire
1
Platelet Activation Assay Protocol
2
Immune Cell Activation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Paraformaldehyde was from Affymetrix (Santa Clara, CA, USA). Anti-OX40L antibodies were from Ancell Corporation (Bayport, MN, USA). Anti-human TACI, anti-human GITRL antibody and the respective isotype control were from R&D Systems (Minneapolis, MN). CD41a-PeCy5, and CD62P-FITC were from BD Pharmingen, CD3-APC/Fire, CD69-PE and CD25-FITC and CD56-PECy7 were obtained from BioLegend. The goat anti-mouse PE conjugate was from Dako (Glostrup, Denmark). Dead cells were excluded using Fixable Aqua (Invitrogen, Carlsbad, CA, USA) after extracellular staining according to the manufacturer’s instructions. Bicoll Separating Solution was purchased from Biochrom AG (Berlin, Germany).
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