Waters uplc tqd mass spectrometer instrument

IR spectra were recorded on Bruker FT-IR, ALPHA-T (Eco-ATR) spectrophotometers, (Bruker Corporation., USA) and the values are expressed in cm^-1. 3220 (N-H stretch), 2865 (C-H Aromatic), 1650 (C=N stretch), 1515 (C-H deform), 1159, 1350 (sym., asym S(=O)₂ stretch). ¹H-NMR and ¹³C-NMR spectra were recorded on Bruker Avance-400, FTNMR spectrometer (Bruker, Tech. Pvt. Ltd., USA) at 400MHz and the chemical shifts are reported in parts per million (δ value), taking TMS (δ 0 ppm for ¹H NMR) as the internal standard: 2.03-2.09 (dd, J_ab: 16.77 Hz, J_ax: 3.58 Hz, 1H, H_a), 2.73-2.78 (dd, J_ab: 3.85 Hz, J_bx: 17.11 Hz, 1H, H_b), 3.81-3.93 (dd, J_ax: 3.50 Hz, J_bx: 17.05 Hz, 1H, H_x), 3.97-4.10 (m, 6H, methyl), 5.23-5.25 (s, 1H, Ar-OH), 6.56-7.90 (m, 11H, Ar). ¹³C NMR (DMSO, ppm): 37.9 (CH₂ pyrazoline), 43.0 (CH pyrazoline), 113.8-146.3 (12CH benzene), 147.5-159.2 (7C benzene), 160.1 (C pyrazoline). Mass spectra were recorded on Waters UPLC-TQD Mass Spectrometer instrument (Waters Corporation, USA) using LC-ESI or APCI-MS Technique; MS (m/z): 473 (M⁺, 100 %). Elemental analysis was performed on Perkin Elmer-2400, Series-II Analyzer (Waltham, Massachusetts, USA). Anal. Calcd. for C₂₃H₂₁ClN₂O₅S: C, 58.41; H, 4.48; N, 5.92. Found: C, 58.39; H, 4.50; N, 5.89.

The chemicals and reagents for synthesis were procured from S. D. Fine Chemicals and Sigma Aldrich, Mumbai, India and the pre-coated TLC sheets were obtained from Merck Chemicals, India and were used as such. Reagent grade solvents were used and were purified and dried by standard methods. Raga's Scientific Microwave System (Ragatech, Pune, Maharashtra, India) was used for the microwave assisted organic synthesis (MAOS). Melting points were determined by open capillary method and are uncorrected. IR spectra were recorded on Bruker FT-IR, ALPHA-T (Eco-ATR) spectrophotometers, (Bruker Corporation, USA) and the values are expressed in cm^-1. ¹H-NMR and ¹³C-NMR spectra were recorded on Bruker Avance-400, FTNMR spectrometer (Bruker, Tech. Pvt. Ltd., USA) at 400 MHz and the chemical shifts are reported in parts per million (δ value), taking TMS (δ 0 ppm for ¹H NMR) as the internal standard. Mass spectra were recorded on Waters UPLC-TQD Mass Spectrometer instrument (Waters Corporation, USA) using LC-ESI or APCI-MS Technique. Elemental analysis was performed on Perkin Elmer-2400, Series-II Analyzer (Waltham, Massachusetts, USA).