miR-483 mimic and pre-miR-483 were provided by Shanghai GenePharma Co., China. HUVECs were transfected with 20 nM of miR-483 mimic or pre-miR-483-3p by the use of Lipofectamine 2000 (Invitrogen), following treatment with 200 nM Ang II (MedChemExpress, MCE) for 24 hours.
Mir 483 mimic
Manufactured by GenePharma
Sourced in China
MiR-483 mimic is a synthetic RNA molecule designed to mimic the function of the natural microRNA miR-483. MicroRNAs are small, non-coding RNA molecules that play a role in regulating gene expression. The MiR-483 mimic is a tool used in research to study the biological functions of miR-483.
Automatically generated - may contain errors
Lab products found in correlation
Ang 2, by MedChemExpress (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Pcdna3.1 nc, by RiboBio (1 mentions)
Nc mimic, by Thermo Fisher Scientific (1 mentions)
Inhibitor nc, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
2 protocols using mir 483 mimic
1
miR-483 modulation of Ang II response
2
miR-483 Modulation in LN229 Cells
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LN229 cells were seeded in 6-well plates and cultured to a density of 70%. The miR-483 mimic, miR-483 inhibitor and negative control were purchased from GenePharma, while pcDNA3.1-SOX3 and pcDNA3.1-NC were purchased from RiboBio (Guangzhou, China). Their sequences were as follows: miR-483 mimic: sense 5ʹ-UCACUCCUCUCCUCCCGUCUU-3ʹ, antisense 5ʹ-GACGGGAGGAGAGGAGUGAUU-3ʹ; NC mimic: sense 5ʹ-UUCUCCGAACGUGUCACGUTT-3ʹ, antisense 5ʹ-ACGUGACACGUUCGGAGAATT-3ʹ; miR-483 inhibitor: 5ʹ-AAGACGGGAGGAGAGGAGUGA-3ʹ; NC inhibitor: 5ʹ-CAGUACUUUUGUGUAGUACAA-3ʹ. Cells were transfected with Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
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