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Mini complete and phosstop inhibitory cocktails

Manufactured by Roche
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Mini-Complete and PhosSTOP are inhibitory cocktails developed by Roche for use in biochemical and cell-based applications. Mini-Complete provides a mix of protease inhibitors, while PhosSTOP contains a combination of phosphatase inhibitors. These products are intended to prevent the degradation of proteins and the dephosphorylation of phosphoproteins during sample preparation and analysis.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions) Peitc, by Thermo Fisher Scientific (2 mentions) Cell dissociation buffer, by Thermo Fisher Scientific (1 mentions) Transit 2020 transfection reagent, by Mirus Bio (1 mentions) Pierce protein g magnetic beads, by Thermo Fisher Scientific (1 mentions) Bradford dye, by Bio-Rad (1 mentions) Blue bio film, by Thomas Scientific (1 mentions) Normal goat igg sc 2028, by Santa Cruz Biotechnology (1 mentions) Ubiquitin amc, by R&D Systems (1 mentions) Alamar blue, by Thermo Fisher Scientific (1 mentions) Uch l3, by R&D Systems (1 mentions) Bortezomib, by LC Laboratories (1 mentions)

Close spelling variants of this product

PhosStop and Complete Mini Complete mini cocktail Complete and PhosSTOP PhosSTOP cocktail Cocktail Complete Complete PhosSTOP Complete Mini PhosSTOP

2 protocols using mini complete and phosstop inhibitory cocktails

1

Ubiquitin-Protease Assay and Reagents

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All chemicals and reagents were from Sigma Aldrich unless otherwise stated. Solvents (except DMSO) were from Fisher (Pittsburg, PA). Other reagents used in this study: PEITC (Acros Organics); Bortezomib (Millennium Pharmaceuticals); Mini-Complete and PhosSTOP inhibitory cocktails (Roche Applied Science); recombinant human K63-linked di-ubiquitin (UC-300), recombinant human His6-USP1/UAF1 complex (E-568; Boston Biochem.); DMEM, glutamax, penicillin/streptomycin (Gibco); trypsin (0.25%), DPBS (Hyclone); cell dissociation buffer (fisher); Bradford dye (Bio-rad); dithiothreitol (GoldBio Tech); PVDF hybond, Amersham ECL Prime WB detection reagent (GE Healthcare Life Sciences); Blue Biofilm (Denville Scientific); ML323 USP1 inhibitor (EMD Millipore); TransIT 2020 transfection reagent (Mirus bio); Pierce protein G magnetic beads (ThermoFisher Scientific); TAMRA-ubiquitin propargylamide (TAMRA-Ub-PA), Cy5-ubiquitin vinyl methyl ester (Cy5-Ub-VME), Biotin-Ahx-Ub-VME, Biotin-Ahx-Ub-PA and Ub-Rh110MP (UbiQ). HA-ubiquitin vinylsulfone (HA-Ub-VS) and HA-Ub-VME were synthesized using standard methods previously described [88 (link)]. The plasmid encoding the HA-Ub(1-75)-intein-chitin binding domain fusion protein was a gift from Prof H. Ploegh of the Whitehead Institute.
Lawson A.P., Bak D.W., Shannon D.A., Long M.J., Vijaykumar T., Yu R., Oualid F.E., Weerapana E, & Hedstrom L. (2017). Identification of deubiquitinase targets of isothiocyanates using SILAC-assisted quantitative mass spectrometry. Oncotarget, 8(31), 51296-51316.
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2

Comprehensive Ubiquitin Assay Protocol

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All chemicals and reagents were from Sigma Aldrich unless otherwise stated. Solvents (except DMSO) were from Fisher (Pittsburg, PA). Other reagents used in this study: G5 isopeptidase inhibitor 1 (50-230-7928, Calbiochem); PEITC (Acros Organics); Bortezomib (Millennium Pharmaceuticals); Mini-Complete and PhosSTOP inhibitory cocktails (Roche Applied Science); Bortezomib (LC laboratories); Alamar Blue® (Invitrogen); USP9x, USP7(catalytic domain), UCH-L3, Ubiquitin-AMC, Suc-Leu-Leu-Val-Tyr-AMC, RAP80 UIM Domains Agarose AM-120, and 20S human proteasome (Boston Biochem); normal goat IgG SC-2028 (Santa Cruz); TAMRA-ubiquitin propargylamide and Cy5-ubiquitin vinyl methyl ester (UbiQ); HA-ubiquitin vinylsulfone and HA-ubiquitin vinyl methyl ester were synthesized using standard methods previously described(16 (link)). The plasmid encoding the HA-1–75Ub-Intein-chitin binding domain fusion protein was a gift from Prof H. Ploegh (Whitehead Institute). BaF3 and BaF3/p210 cells were provided by Nathaniel Gray (Harvard University)
Lawson A.P., Long M.J., Coffey R.T., Qian Y., Weerapana E., El Oualid F, & Hedstrom L. (2015). Naturally occurring isothiocyanates exert anticancer effects by inhibiting deubiquitinating enzymes. Cancer research, 75(23), 5130-5142.
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