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Anti cd3 cd28 beads

Manufactured by BD
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Anti-CD3/CD28 beads are a type of lab equipment used in cell culture and immunological research. They are small, magnetic beads coated with antibodies targeting the CD3 and CD28 cell surface receptors. These beads are designed to activate and stimulate T cells, a key component of the immune system, by cross-linking these receptors.

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Lab products found in correlation

Cytometric bead array human th1 th2 th17 cytokine kit, by BD (2 mentions) Anti cd3 cd28 beads, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Anti-CD3/CD28 coated beads CD3/CD28 beads Anti-CD3/CD28 Anti-CD28 Anti-CD3

2 protocols using anti cd3 cd28 beads

1

Modulation of T cell activation

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Peripheral blood lymphocytes from two donors were stimulated with anti-CD3/CD28 beads (ThermoFisher Scientific) at a 2:1 bead to T cell ratio. Cells were cultured for a two week period in RPMI with 5% HS, IL-2 (50 IU/mL), IL-15 (10 ng/mL) in the presence of SEN177 (10 μM) or control (DMSO). Every 2 to 3 days, cells were counted and re-plated at a density of 0.25 × 106 cells/mL. Surface expression of phenotypic markers was analysed on day 0, 5, 10 (data not shown) and 14 with the indicated antibodies. On day 14, T cells were re-stimulated with anti-CD3/CD28 beads (1:1 bead to T cell ratio), supernatants were collected 38 hours after stimulation, and levels of IL-2, IL-4, IL-6, IL-10, IFNγ, IL17A and TNFα were measured using the Cytometric Bead Array Human Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA), according to the manufacturer’s protocol.
Logtenberg M.E., Jansen J.H., Raaben M., Toebes M., Franke K., Brandsma A.M., Matlung H.L., Fauster A., Gomez-Eerland R., Bakker N.A., van der Schot S., Marijt K.A., Verdoes M., Haanen J.B., van den Berg J.H., Neefjes J., van den Berg T.K., Brummelkamp T.R., Leusen J.H., Scheeren F.A, & Schumacher T.N. (2019). Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and target for cancer immunotherapy. Nature medicine, 25(4), 612-619.
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2

Modulation of T cell activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood lymphocytes from two donors were stimulated with anti-CD3/CD28 beads (ThermoFisher Scientific) at a 2:1 bead to T cell ratio. Cells were cultured for a two week period in RPMI with 5% HS, IL-2 (50 IU/mL), IL-15 (10 ng/mL) in the presence of SEN177 (10 μM) or control (DMSO). Every 2 to 3 days, cells were counted and re-plated at a density of 0.25 × 106 cells/mL. Surface expression of phenotypic markers was analysed on day 0, 5, 10 (data not shown) and 14 with the indicated antibodies. On day 14, T cells were re-stimulated with anti-CD3/CD28 beads (1:1 bead to T cell ratio), supernatants were collected 38 hours after stimulation, and levels of IL-2, IL-4, IL-6, IL-10, IFNγ, IL17A and TNFα were measured using the Cytometric Bead Array Human Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA), according to the manufacturer’s protocol.
Logtenberg M.E., Jansen J.H., Raaben M., Toebes M., Franke K., Brandsma A.M., Matlung H.L., Fauster A., Gomez-Eerland R., Bakker N.A., van der Schot S., Marijt K.A., Verdoes M., Haanen J.B., van den Berg J.H., Neefjes J., van den Berg T.K., Brummelkamp T.R., Leusen J.H., Scheeren F.A, & Schumacher T.N. (2019). Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and target for cancer immunotherapy. Nature medicine, 25(4), 612-619.
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