Rabbit monoclonal antibody against PKCα (Phospho T638) (1:500 dilution) and rabbit polyclonal antibodies against PKCα (1:2000 dilution), NF-κB p65 (1:2000 dilution), and Histone H3 (1:3000 dilution) were purchased from Abcam (Cambridge, MA, USA). The rabbit polyclonal antibody against α-Tubulin (1:5000 dilution) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Tumor necrosis factor (TNF) -α was purchased from R&D systems (Minneapolis, MN, USA). It was reconstituted at 100 μg/ml in sterile PBS and stored at −80 °C; the TNF-α solution was diluted in serum-free medium to a concentration of 10 ng/ml when added to the cells. BAY 11–7082, Gö6976 and Sotrastaurin were purchased from Selleckchem (Houston, TX, USA). They were reconstituted in DMSO, and when added to the cells, 10 μL of DMSO was added per 1.0 ml of media as the control. Phorbol 12-myristate 13-acetate (PMA) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Tumor necrosis factor (TNF) -α was purchased from R&D systems (Minneapolis, MN, USA). It was reconstituted at 100 μg/ml in sterile PBS and stored at −80 °C; the TNF-α solution was diluted in serum-free medium to a concentration of 10 ng/ml when added to the cells. BAY 11–7082, Gö6976 and Sotrastaurin were purchased from Selleckchem (Houston, TX, USA). They were reconstituted in DMSO, and when added to the cells, 10 μL of DMSO was added per 1.0 ml of media as the control. Phorbol 12-myristate 13-acetate (PMA) was purchased from Sigma-Aldrich (St. Louis, MO, USA).