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Ncam1

Manufactured by Cedarlane

Ncam1 is a laboratory equipment product used for cellular imaging and analysis. It is a high-performance camera system designed to capture and record live-cell dynamics with high spatial and temporal resolution.

Automatically generated - may contain errors

2 protocols using ncam1

1

Characterization of Tumor Cell Populations

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Tumors were dissected from the lungs of TKO Hes1GFP/+ mice approximately 5–7 months after tumor induction and digested as previously described40 (link). The antibodies used were: CD45-PE-Cy7 (eBioscience, clone 30-F11, 1:100), CD31-PE-Cy7 (eBioscience, clone 390, 1:100), TER-119-PE-Cy7 (eBioscience, clone TER-119, 1:100), CD24-APC (eBioscience, clone M1/69, 1:200), Ncam1 (Cedarlane, clone H28-123-16, 1:100), anti-rat-IgG2a-PE (eBioscience, clone r2a-21B2, 1:200), EpCam (eBioscience, clone G8.8, 1:100), CD44-APC-Cy7 (BioLegend, clone IM7, 1:100). 1 μg/mL 7-aminoactinomycin D (Invitrogen) or DAPI was used to label dead cells.
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2

Characterization of Tumor Cell Populations

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors were dissected from the lungs of TKO Hes1GFP/+ mice approximately 5–7 months after tumor induction and digested as previously described40 (link). The antibodies used were: CD45-PE-Cy7 (eBioscience, clone 30-F11, 1:100), CD31-PE-Cy7 (eBioscience, clone 390, 1:100), TER-119-PE-Cy7 (eBioscience, clone TER-119, 1:100), CD24-APC (eBioscience, clone M1/69, 1:200), Ncam1 (Cedarlane, clone H28-123-16, 1:100), anti-rat-IgG2a-PE (eBioscience, clone r2a-21B2, 1:200), EpCam (eBioscience, clone G8.8, 1:100), CD44-APC-Cy7 (BioLegend, clone IM7, 1:100). 1 μg/mL 7-aminoactinomycin D (Invitrogen) or DAPI was used to label dead cells.
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