Log In Sign up
The largest database of trusted experimental protocols

Anti mouse tlr7

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Anti-mouse TLR7 is a laboratory reagent used to detect and quantify the expression of Toll-like receptor 7 (TLR7) in mouse cells and tissues. TLR7 is an important immune receptor that recognizes single-stranded RNA and plays a crucial role in the body's response to viral infections. The Anti-mouse TLR7 can be used in various research applications, such as flow cytometry, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA).

Automatically generated - may contain errors

Lab products found in correlation

Lysis buffer, by Cell Signaling Technology (2 mentions) Anti mouse igg isotype control, by Santa Cruz Biotechnology (2 mentions) Anti mouse myd88, by Santa Cruz Biotechnology (2 mentions)

2 protocols using anti mouse tlr7

1

TLR7-MyD88 Co-immunoprecipitation in BMDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Co-immunoprecipitation experiments were performed using BMDCs seeded in 6-well plates at a final concentration of 1×106 cells/mL in RPMI-complete media. Cells were incubated with 10 μg/mL Gardiquimod for 15 minutes at 37C with 5% CO2. Cells were harvested and lysed in 1× lysis buffer (Cell Signaling). Lysates were incubated with anti-mouse TLR7 (Fisher Scientific) or anti-mouse IgG isotype control (Santa Cruz Biotech) for 24 h at 4C. Supernatants were incubated with Protein G agarose beads overnight at 4C. Precipitated proteins are eluted, separated by SDS-PAGE and immunoblotted with anti-mouse MyD88 (Santa Cruz Biotech).
Ramirez-Ortiz Z.G., Prasad A., Griffith J.W., Pendergraft WF I.I.I., Cowley G.S., Root D.E., Tai M., Luster A.D., Khoury J.E., Hacohen N, & Means T.K. (2015). TREML4 amplifies TLR7-mediated signaling during antiviral responses and autoimmunity. Nature immunology, 16(5), 495-504.
+ Open protocol
+ Expand
2

TLR7-MyD88 Co-immunoprecipitation in BMDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Co-immunoprecipitation experiments were performed using BMDCs seeded in 6-well plates at a final concentration of 1×106 cells/mL in RPMI-complete media. Cells were incubated with 10 μg/mL Gardiquimod for 15 minutes at 37C with 5% CO2. Cells were harvested and lysed in 1× lysis buffer (Cell Signaling). Lysates were incubated with anti-mouse TLR7 (Fisher Scientific) or anti-mouse IgG isotype control (Santa Cruz Biotech) for 24 h at 4C. Supernatants were incubated with Protein G agarose beads overnight at 4C. Precipitated proteins are eluted, separated by SDS-PAGE and immunoblotted with anti-mouse MyD88 (Santa Cruz Biotech).
Ramirez-Ortiz Z.G., Prasad A., Griffith J.W., Pendergraft WF I.I.I., Cowley G.S., Root D.E., Tai M., Luster A.D., Khoury J.E., Hacohen N, & Means T.K. (2015). TREML4 amplifies TLR7-mediated signaling during antiviral responses and autoimmunity. Nature immunology, 16(5), 495-504.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!