Tcs 264 sp5x system

HeLa cells were cultured in DMEM medium containing 10% FBS, 1% penicillin/streptomycin (complete DMEM) in 5% CO₂ at 37 °C. HeLa cells were seeded in a μ-slide eight well plate for 24 h, and then the medium was refreshed. The cells were stained with wheat germ agglutinin Alexa Fluor-TM 488 conjugate and Hoechst 33342 to show the cell membrane, as well as with propidium iodide (PI) to show the real-time enhanced permeability of cell membranes. The cells were washed with PBS twice. Thereafter, the cells were treated with AIE/Au nanomotors (25 µg ml⁻¹). Immediately, cells were subjected to a two-photon confocal NIR laser and the fluorescence images of the cells were captured using a Leica TCS 264 SP5X system.

HeLa cells were cultured in DMEM medium containing 10% FBS, 1% penicillin/streptomycin (complete DMEM) in 5% CO₂ at 37 °C. HeLa cells were seeded in a μ-slide eight well plate for 24 h, and then the medium was refreshed. The cells were subsequently loaded with CM-H2DCF for 0.5 h and stained with Hoechst 33342 for 10 min. Then the cells were washed and treated with AIE/Au nanomotors (25 µg ml⁻¹). Immediately, the cells were subjected to a two-photon confocal NIR laser and the fluorescence images of the cells were captured using a Leica TCS 264 SP5X system.

HeLa cells were cultured in DMEM medium containing 10% FBS, 1% penicillin/streptomycin (complete DMEM) in 5% CO₂ at 37 °C. HeLa cells were seeded in a μ-slide 8 well plate for 24 h, and then the medium was refreshed. Then, the cells were stained with Hoechst 33342 for 10 min. Thereafter, the cells were washed and incubated with calcein for live-cell staining, and PI for dead cell staining for 10 min. Next, the cells were treated with AIE/Au nanomotors (25 µg ml⁻¹). Immediately, the cells were subjected to a two-photon confocal NIR laser and the fluorescence images of the cells were captured using a Leica TCS 264 SP5X system.