Mic a b

TRIzol was purchased from Invitrogen Life Technologies (Carlsbad, CA, USA) and M-MLV reverse transcriptase was purchased from Promega Corporation (Madison, WI, USA). MIC A/B and NKG2D mouse anti-human monoclonal antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), hypoxia-inducible factor 1-α (HIF-1α) rabbit anti-human polyclonal antibody was purchased from Boster Systems Inc. (Pleasanton, CA, USA) and glyceryl trinitrate (GTN) transdermal patches were purchased from Schwarz Pharma (Brussels, Belgium).

Sub-confluent cells in normal culture media were transfected or infected with control or specific siRNA, shRNA vectors, or virus encoding the indicated molecules for 48 or 24 h, respectively, in the presence of vehicle or TSAHC [26 (link)] treatment. Animal liver tissues were harvested for whole-cell or tissue extraction using a modified RIPA buffer, as previously described [17 (link), 21 (link)]. The primary antibodies (generally at 1:1000 dilution) used included antibodies against laminins, pY³⁹⁷FAK, MICA/B (Abcam, Cambridge, UK), p-ERKs, ERKs (Cell Signaling Technology. Danvers, MA, USA), pY⁷⁰⁵STAT3 (Millipore, Billerica, MA, USA), β-actin, α-tubulin, SLAMF7 (CS1), MICA/B, STAT3, pY⁵⁷⁷FAK (Santa Cruz Biotechnology, Santa Cruz, CA, USA), FAK (BD Transduction Laboratories, Bedford, MA, USA), collagen I (Acris Antibodies, Herford, Germany), and TM4SF5 [17 (link)]. The TM4SF5_C-ter (epitope region of RKKQDTPH¹⁹⁷) antibody was custom designed (Pro-Sci, Poway, CA, USA).