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Rpmi 1640 no glucose media

Manufactured by Thermo Fisher Scientific
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RPMI 1640 no glucose media is a cell culture medium that does not contain glucose. It is commonly used for the in vitro cultivation of a variety of cell types, including mammalian and human cell lines.

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Lab products found in correlation

Rpmi 1640 media, by Thermo Fisher Scientific (4 mentions) Tetracycline free fetal bovine serum, by Takara Bio (2 mentions) β mercaptoethanol, by Merck Group (2 mentions) Thapsigargin, by Merck Group (2 mentions) Tunicamycin, by Merck Group (2 mentions)

2 protocols using rpmi 1640 no glucose media

1

Chemically Inducing ER Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tunicamycin and thapsigargin (Sigma) were used at the concentrations specified in the figure legends. For chemical ER stress experiments involving inducible overexpression or knockdown of Wfs1, RPMI 1640 media (ThermoFisher Scientific) was supplemented with tetracycline-free fetal bovine serum (Clontech) and β-mercaptoethanol (Sigma). For glucotoxic ER stress experiments, RPMI 1640 no glucose media (ThermoFisher Scientific, cat no 11879020) was supplemented with D-glucose (Gibo) as specified in figure legends.
Abreu D., Asada R., Revilla J.M., Lavagnino Z., Kries K., Piston D.W, & Urano F. (2020). Wolfram syndrome 1 gene regulates pathways maintaining beta cell health and survival. Laboratory investigation; a journal of technical methods and pathology, 100(6), 849-862.
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2

Chemically Inducing ER Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tunicamycin and thapsigargin (Sigma) were used at the concentrations specified in the figure legends. For chemical ER stress experiments involving inducible overexpression or knockdown of Wfs1, RPMI 1640 media (ThermoFisher Scientific) was supplemented with tetracycline-free fetal bovine serum (Clontech) and β-mercaptoethanol (Sigma). For glucotoxic ER stress experiments, RPMI 1640 no glucose media (ThermoFisher Scientific, cat no 11879020) was supplemented with D-glucose (Gibo) as specified in figure legends.
Abreu D., Asada R., Revilla J.M., Lavagnino Z., Kries K., Piston D.W, & Urano F. (2020). Wolfram syndrome 1 gene regulates pathways maintaining beta cell health and survival. Laboratory investigation; a journal of technical methods and pathology, 100(6), 849-862.
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