A Thermo Scientific Genesys UV/visible spectrophotometer was used to record the UV-visible absorption spectrum of diluted Ag2S-NP samples. The absorption intensity was normalized to a maximum of 1 across all samples.
Genesys uv visible spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Genesys UV/visible spectrophotometer is a laboratory instrument used to measure the absorption of light by a sample across the ultraviolet and visible light spectrum. It is designed to quantify the concentration of a specific substance in a solution by analyzing the amount of light absorbed at a particular wavelength.
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4 protocols using genesys uv visible spectrophotometer
1
UV-Vis Absorption Spectrum of Ag2S-NPs
2
Quantifying Total Phenolics in Grape Puree
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Total phenolic content was determined via Folin–Ciocalteau colorimetric assay according to the method reported by Waterhouse [52 ] with minor modifications. Generally, 20 μL of diluted extract was mixed with 1580 μL DI water and 100 μL Folin–Ciocalteau reagent. The mixture was vortexed and incubated at room temperature for 6 min. After incubation, 300 μL of 20% (w/v) sodium carbonate solution was added and gently vortexed before incubating at room temperature for 2 h in the dark. Absorbance was measured at 765 nm using a Genesys UV-visible Spectrophotometer (10S, Thermo Fisher Scientific, Waltham, MA, USA). Gallic acid solutions (0 to 500 mg/L) were used to determine the standard curve. Results were expressed as gallic acid equivalent (GAE) mg/g of fresh weight of whole Concord grape puree. Calculation was carried out according to Equation (5):
3
UV-Vis Absorption Spectra of YbNPs
4
Quantification of Azadirachtin in Neem by UPC2
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Azadirachtin in Neem was determined by UltraPerformance Convergence Chromatography (UPC2) (Waters ACQUITY, Milford MA, USA) equipped with a photodiode array detection and a C-18 column (Waters 1.0 × 100 mm, 1.7 μm) using supercritical CO2 and methanol as the mobile phase (Flow rate = 1.5 mL·min−1), convergence pressure of 1500 psi, and column temperature of 30 °C. The sample was dissolved in methanol: Petroleum ether of 5 °C [36 (link)]. Inhibition of radical scavenging activity (RSA) was determined spectrophotometrically with Neem concentrations from 5 to 25 µg·mL−1 with freshly prepared DPPH (0.1 mM/methanol) (Sigma-Aldrich, St. Louis, MO, USA). Test tubes were stirred in a vortex followed by incubation at 30 °C for 30 min in darkness. Absorbance at 517 nm was measured in a Genesys UV-visible spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) by triplicate. RSA (%) was determined from Equation (1), considering the absorbance of ethanol as the control (A0) and the sample (AX):
Experimental data were fitted to Probit statistical analysis in the NCSS® program [37 ]. The IC50 value was the concentration of N that inhibited 50% of DPPH [38 ].
Experimental data were fitted to Probit statistical analysis in the NCSS® program [37 ]. The IC50 value was the concentration of N that inhibited 50% of DPPH [38 ].
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