Rotisolv

Manufactured by Carl Roth

Sourced in Germany

ROTISOLV is a multipurpose solvent suitable for various laboratory applications. It provides consistent performance and purity to meet the needs of scientific research and analysis.

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Lab products found in correlation

Rotipuran, by Carl Roth (1 mentions) Gc ultra grade, by Carl Roth (1 mentions) N ethyl n 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Merck Group (1 mentions) Oleic acid, by Thermo Fisher Scientific (1 mentions) Recl3 6h2o, by Merck Group (1 mentions) Recl3 6 h2o, by Thermo Fisher Scientific (1 mentions) Ammonium fluoride, by Merck Group (1 mentions) Formic acid, by Carl Roth (1 mentions) Simplicity system, by Merck Group (1 mentions) Acetonitrile, by Carl Roth (1 mentions) 0.22 μm membrane filter, by Merck Group (1 mentions) Cellpure, by Carl Roth (1 mentions) Hydrochloric acid (hcl), by AppliChem (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

7 protocols using rotisolv

Wastewater Bioassay Extraction Protocol

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Wastewater samples (1-5 L) were collected as 24 h composite samples. Surface water samples were collected as 1 L grab samples. Aqueous samples were kept in amber glass bottles at 4 °C until testing (max. 3 d after sampling) or extracted on site directly after sampling by an optimized solid phase extraction (SPE) method (Abbas et al., in prep.) .
The procedure in brief: Prior to SPE, 500 mL of each sample were filtered through Whatman GF6 filters (pore size < 1 µm), acidified with sulfuric acid (3.5 M, picograde) to pH 2.5 and extracted using Telos C18/ENV columns (Kinesis). A SPE blank was included by applying the same procedure to an analytically pure groundwater (GW) sample. SPE columns were eluted with 5 x 2 mL methanol (Carl Roth, Rotisolv, Ultra LC-MS) and 5 x 2 mL acetone (Carl Roth, Rotisolv, GC Ultra). 100 µL dimethyl sulfoxide (DMSO, Sigma-Aldrich, 99.5%) was added to each extract. The methanol/acetone was evaporated under a gentle nitrogen stream. This resulted in a 5000 fold increase in solute concentration (5000x). SPE extracts were kept at -20 °C until bioassay analysis.

Abbas A., Valek L., Schneider I., Bollmann A., Knopp G., Seitz W., Schulte-Oehlmann U., Oehlmann J, & Wagner M. (2018). Ecotoxicological impacts of surface water and wastewater from conventional and advanced treatment technologies on brood size, larval length, and cytochrome P450 (35A3) expression in Caenorhabditis elegans. Environmental science and pollution research international, 25(14).

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Peptide Separation by Gradient Elution

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The solvent used for mobile phase A was water with 0.1% formic acid and the mobile phase B was acetonitrile with 0.1% formic acid (both LC-MS grade, ROTISOLV^®, ≥99.9%, Carl Roth). The separation of the peptides followed on the analytical column with a linear gradient with increasing concentration of mobile phase B. The initial conditions were 97% A and 3% B at a flow rate of 5 µL/min. The linear gradient used was 3–25% B in 38 min. Within the following 5 min, the mobile phase B was increased to 32% and was further increased to 80% within 2 min, lasting for 3 min. At minute 49, the mobile phases were changed to the initial condition with 97% A and 3% B within 8 min.

Hünnefeld M., Viets U., Sharma V., Wirtz A., Hardy A, & Frunzke J. (2021). Genome Sequence of the Bacteriophage CL31 and Interaction with the Host Strain Corynebacterium glutamicum ATCC 13032. Viruses, 13(3), 495.

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Preparation of Polymeric Micro- and Nanoparticles

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To prepare the MP and NP, the following compounds were used: AMCA (~90%, Sigma-Aldrich, Steinheim, Germany); N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (≥95%, Sigma-Aldrich); acetonitrile (≥99.5%, pharmaceutical quality, Sigma-Aldrich); ethanol (≥95%, Rotipuran, Carl Roth GmbH, Karlsruhe, Germany); acetone (ROTISOLV^® ≥99.9%, GC Ultra Grade, Carl Roth GmbH); PLGA 50:50 (RESOMER^® RG 502H, MW 7,000–17,000 g/mol, pharmaceutical quality, kindly provided by Boehringer Ingelheim, Ingelheim am Rhein, Germany); poly(ethylene glycol)-block-PLGA (PEG-PLGA) 50:50 (RESOMER^® RGP d 50155, 5 kDa PEG, pharmaceutical quality, Evonik, Essen, Germany); polyvinyl alcohol (Mowiol 4–88, 31,000 g/mol, Kuraray Specialities Europe, Frankfurt, Germany); ultrapure chitosan chloride (30–400 kDa, pharmaceutical quality, Heppe Medical Chitosan, Halle, Germany); and ethyl acetate (≥99.5%, p.a., ISO, Carl Roth GmbH).

Klinger-Strobel M., Ernst J., Lautenschläger C., Pletz M.W., Fischer D, & Makarewicz O. (2016). A blue fluorescent labeling technique utilizing micro- and nanoparticles for tracking in LIVE/DEAD® stained pathogenic biofilms of Staphylococcus aureus and Burkholderia cepacia. International Journal of Nanomedicine, 11, 575-583.

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Synthesis of Rare Earth-Doped Nanocrystals

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All chemicals were used as received without previous purification. From Sigma Aldrich (St. Louis, MO, USA) were purchased: RECl₃·6H₂O (RE:Eu³⁺, Pr³⁺, Y³⁺, Yb³⁺, purity >99.99%) and ammonium fluoride (NH₄F, ≥99.99%). From Alfa Aesar (Kandel, Germany) were purchased: RECl₃·6H₂O (RE: Gd³⁺, Nd³⁺, purity > 99.99%) and oleic acid (OA, 90%). From Carl Roth (Karlsruhe, Germany) were purchased: cyclohexane (ROTISOLV^® ≥ 99.9%), ethanol (≥99.8%, 1% MEK) and sodium oleate (NaOA, 90%). The solvent Therminol^® 66 was bought from FRAGOL GmbH + Co KG (Mülheim, Germany).

Bastian P.U., Robel N., Schmidt P., Schrumpf T., Günter C., Roddatis V, & Kumke M.U. (2021). Resonance Energy Transfer to Track the Motion of Lanthanide Ions—What Drives the Intermixing in Core-Shell Upconverting Nanoparticles?. Biosensors, 11(12), 515.

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Quantification of Hydromorphone in Serum

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Hydromorphone (1 mg/mL in methanol) and internal standard (IS) hydromorphone-D3 were purchased from LGC Standards (Wesel, Germany). Water for the mobile phase (ROTISOLV®), acetonitrile, methanol, formic acid, and ammonia (30%) were purchased from Roth (Karlsruhe, Germany); all chemicals and solvents were high-performance liquid-chromatography (HPLC) grade. Deionized water for the solid-phase extraction was prepared with a Simplicity® system from Millipore (Schwalbach, Germany). Lyophilized drug-free human serum was obtained from Bio-Rad Laboratories (Feldkirchen, Germany), and each vial was reconstituted with 10 mL of LC-MS/MS grade water for use.

Wehrfritz A., Schmidt S., Ihmsen H., Schüttler J, & Jeleazcov C. (2022). Long-Term Stability of Hydromorphone in Human Plasma Frozen at −20°C for Three Years Quantified by LC-MS/MS. International Journal of Analytical Chemistry, 2022, 3645048.

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Extraction and Analysis of Bioactive Compounds from Berries

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For evaluation of the content of bioactive compounds, the extracts were prepared by homogenizing the berries with a medium at a 1:10 ratio of plant material in a porcelain mortar and pestle. The medium consisted of 90% aqueous methanol (Rotisolv, Roth, Karlsruhe, Germany) acidified with 0.1 N hydrochloric acid (Fluka Analytical, Munich, Germany). The homogenate was stirred with a magnetic stirrer for 30 min. The sealed homogenates were stirred with a magnetic stirrer (MM 2A, Czech) for 16 h in the dark at 4 °C, and the precipitate was then removed using a water vacuum pump with a 0.22 μm membrane filter (Millipore, Darmstadt, Germany).
The berries were homogenized in 50% methanol for ascorbic acid analysis. The homogenate was centrifuged for 15 min at 4 °C at a speed of 3000 rpm (centrifuge MPW-351 R, MPW Med. instruments, Warsaw, Poland). Prior to analysis, the extracts were stored for 16 h at 4 °C in the dark.

Mockevičiūtė R., Jurkonienė S., Gavelienė V., Jankovska-Bortkevič E., Šocik B., Armalytė G, & Budrys R. (2022). Effects Induced by the Agricultural Application of Probiotics on Antioxidant Potential of Strawberries. Plants, 11(6), 831.

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Preparation of Phosphate Buffered Saline

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Phosphate buffered saline (PBS) with varying pH was prepared by mixing 1 M stock solutions (Table S1), then diluting to the final volume of 10 mL. The pH was determined with a digital pH meter (UltraBASIC, Denver Instruments, US). The 1 M stock solutions of NaCl (CELLPURE®, Carl ROTH, Germany) and NaH 2 PO 4 were prepared by dissolving each salt in high-performance liquid chromatography (HPLC)-grade water (ROTISOLV®, Carl ROTH, Germany). 1 M stock solutions of NaOH (Fluka, Germany) and HCl (AppliChem, Germany) were used as acquired. Saline was prepared by diluting 440 µL of 1 M NaCl stock solution with HPLC-water to a total volume of 10 mL.

Kunnas P., Moradi M.A., Sommerdijk N, & de Jonge N. (2022). Strategy for optimizing experimental settings for studying low atomic number colloidal assemblies using liquid phase scanning transmission electron microscopy. Ultramicroscopy, 240.

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